Rapid phosphorylation of MAP kinase-like proteins in two species of Arctic kelps in response to temperature and UV radiation stress
Mitogen-Activated Protein Kinases (MAPKs) are a group of cytoplasmic phosphoproteins that constitute the central core of the signalling network to respond to stress in most organisms. Their role in stress responses has been extensively studied in organisms from yeast to humans, and recently, their p...
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Online Access: | https://epic.awi.de/id/eprint/32262/ https://hdl.handle.net/10013/epic.41242 |
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ftawi:oai:epic.awi.de:32262 2023-05-15T14:26:42+02:00 Rapid phosphorylation of MAP kinase-like proteins in two species of Arctic kelps in response to temperature and UV radiation stress Parages, María L. Heinrich, Sandra Wiencke, Christian Jimenez, Carlos 2013 https://epic.awi.de/id/eprint/32262/ https://hdl.handle.net/10013/epic.41242 unknown PERGAMON-ELSEVIER SCIENCE LTD Parages, M. L. , Heinrich, S. , Wiencke, C. and Jimenez, C. (2013) Rapid phosphorylation of MAP kinase-like proteins in two species of Arctic kelps in response to temperature and UV radiation stress , Environmental and Experimental Botany, online . doi:10.1016/j.envexpbot.2013.02.005 <https://doi.org/10.1016/j.envexpbot.2013.02.005> , hdl:10013/epic.41242 EPIC3Environmental and Experimental Botany, PERGAMON-ELSEVIER SCIENCE LTD, online, ISSN: 0098-8472 Article isiRev 2013 ftawi https://doi.org/10.1016/j.envexpbot.2013.02.005 2021-12-24T15:38:23Z Mitogen-Activated Protein Kinases (MAPKs) are a group of cytoplasmic phosphoproteins that constitute the central core of the signalling network to respond to stress in most organisms. Their role in stress responses has been extensively studied in organisms from yeast to humans, and recently, their presence has also been described in higher plants as well as in micro- and macroalgae. In this study, we demonstrate via short experiments (1 h in duration), the rapid activation of two MAPKs similar to p38 and JNK of mammalian cells, in the Arctic kelps Laminaria solidungula and Saccharina latissima exposed to temperature and UV stress. The molecular mass of p38 is 40 KDa in L. solidungula and 42 KDa in S. latissima, while two JNKs were detected in both species, of 36 and 42 KDa in L. solidungula, and 36 and 40 KDa in S. latissima. These MAPKs are highly phosphorylated in response to temperature and UV light. In S. latissima, both p38 and the JNK showed higher phosphorylation at 2C than at 7C, while the reverse response was shown for L. solidungula. In addition, a significant increase in phosphorylation of both kinases was found following exposure to UV radiation (UVR). Exposure to PAR+UVA+UVB induced higher phosphorylation than PAR+UVA in L. solidungula, especially at 7C. In S. latissima, this response occurred only with JNK, and no differences in p38 phosphorylation between PAR+UVA and PAR+UVA+UVB at any temperature were observed. These results indicate the possible participation of MAPK-like proteins in response to stress in Arctic kelps, and that their activation is species-specific. Article in Journal/Newspaper Arctic Arctic Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Arctic Environmental and Experimental Botany 91 30 37 |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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ftawi |
language |
unknown |
description |
Mitogen-Activated Protein Kinases (MAPKs) are a group of cytoplasmic phosphoproteins that constitute the central core of the signalling network to respond to stress in most organisms. Their role in stress responses has been extensively studied in organisms from yeast to humans, and recently, their presence has also been described in higher plants as well as in micro- and macroalgae. In this study, we demonstrate via short experiments (1 h in duration), the rapid activation of two MAPKs similar to p38 and JNK of mammalian cells, in the Arctic kelps Laminaria solidungula and Saccharina latissima exposed to temperature and UV stress. The molecular mass of p38 is 40 KDa in L. solidungula and 42 KDa in S. latissima, while two JNKs were detected in both species, of 36 and 42 KDa in L. solidungula, and 36 and 40 KDa in S. latissima. These MAPKs are highly phosphorylated in response to temperature and UV light. In S. latissima, both p38 and the JNK showed higher phosphorylation at 2C than at 7C, while the reverse response was shown for L. solidungula. In addition, a significant increase in phosphorylation of both kinases was found following exposure to UV radiation (UVR). Exposure to PAR+UVA+UVB induced higher phosphorylation than PAR+UVA in L. solidungula, especially at 7C. In S. latissima, this response occurred only with JNK, and no differences in p38 phosphorylation between PAR+UVA and PAR+UVA+UVB at any temperature were observed. These results indicate the possible participation of MAPK-like proteins in response to stress in Arctic kelps, and that their activation is species-specific. |
format |
Article in Journal/Newspaper |
author |
Parages, María L. Heinrich, Sandra Wiencke, Christian Jimenez, Carlos |
spellingShingle |
Parages, María L. Heinrich, Sandra Wiencke, Christian Jimenez, Carlos Rapid phosphorylation of MAP kinase-like proteins in two species of Arctic kelps in response to temperature and UV radiation stress |
author_facet |
Parages, María L. Heinrich, Sandra Wiencke, Christian Jimenez, Carlos |
author_sort |
Parages, María L. |
title |
Rapid phosphorylation of MAP kinase-like proteins in two species of Arctic kelps in response to temperature and UV radiation stress |
title_short |
Rapid phosphorylation of MAP kinase-like proteins in two species of Arctic kelps in response to temperature and UV radiation stress |
title_full |
Rapid phosphorylation of MAP kinase-like proteins in two species of Arctic kelps in response to temperature and UV radiation stress |
title_fullStr |
Rapid phosphorylation of MAP kinase-like proteins in two species of Arctic kelps in response to temperature and UV radiation stress |
title_full_unstemmed |
Rapid phosphorylation of MAP kinase-like proteins in two species of Arctic kelps in response to temperature and UV radiation stress |
title_sort |
rapid phosphorylation of map kinase-like proteins in two species of arctic kelps in response to temperature and uv radiation stress |
publisher |
PERGAMON-ELSEVIER SCIENCE LTD |
publishDate |
2013 |
url |
https://epic.awi.de/id/eprint/32262/ https://hdl.handle.net/10013/epic.41242 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic |
genre_facet |
Arctic Arctic |
op_source |
EPIC3Environmental and Experimental Botany, PERGAMON-ELSEVIER SCIENCE LTD, online, ISSN: 0098-8472 |
op_relation |
Parages, M. L. , Heinrich, S. , Wiencke, C. and Jimenez, C. (2013) Rapid phosphorylation of MAP kinase-like proteins in two species of Arctic kelps in response to temperature and UV radiation stress , Environmental and Experimental Botany, online . doi:10.1016/j.envexpbot.2013.02.005 <https://doi.org/10.1016/j.envexpbot.2013.02.005> , hdl:10013/epic.41242 |
op_doi |
https://doi.org/10.1016/j.envexpbot.2013.02.005 |
container_title |
Environmental and Experimental Botany |
container_volume |
91 |
container_start_page |
30 |
op_container_end_page |
37 |
_version_ |
1766299998111137792 |