Characteristics of digestive enzymes of calanoid copepod species from different latitudes in relation to temperature, pH and food
In calanoid copepods it is poorly understood how enzymatic activities and patterns are affected by abiotic and biotic factors. Such knowledge, however, is crucial to assess metabolic functioning and performance of organisms in different habitats. Therefore, our study focuses on digestive enzyme acti...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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2012
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Online Access: | https://epic.awi.de/id/eprint/31896/ https://hdl.handle.net/10013/epic.40641 |
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ftawi:oai:epic.awi.de:31896 2024-09-15T18:00:43+00:00 Characteristics of digestive enzymes of calanoid copepod species from different latitudes in relation to temperature, pH and food Freese, Daniela Kreibich, Tobias Niehoff, Barbara 2012 https://epic.awi.de/id/eprint/31896/ https://hdl.handle.net/10013/epic.40641 unknown Freese, D. , Kreibich, T. and Niehoff, B. (2012) Characteristics of digestive enzymes of calanoid copepod species from different latitudes in relation to temperature, pH and food , Comparative Biochemistry and Physiology, Part B, 162 (4), pp. 66-72 . doi:10.1016/j.cbpb.2012.04.007 <https://doi.org/10.1016/j.cbpb.2012.04.007> , hdl:10013/epic.40641 EPIC3Comparative Biochemistry and Physiology, Part B, 162(4), pp. 66-72 Article peerRev 2012 ftawi https://doi.org/10.1016/j.cbpb.2012.04.007 2024-06-24T04:06:16Z In calanoid copepods it is poorly understood how enzymatic activities and patterns are affected by abiotic and biotic factors. Such knowledge, however, is crucial to assess metabolic functioning and performance of organisms in different habitats. Therefore, our study focuses on digestive enzyme activities in relation to temperature,pH and food in the Arctic species Calanus glacialis and in Centropages hamatus and Temora longicornis from the North Sea. Enzyme activities were measured over a range from 0 to 70 °C (lipases/esterases, proteinases)and pH 5 to 9 (proteinases). In all species, relative proteinases activity peaked at 40/50 °C and pH 6; relative lipases/esterases activity peaked at 30 °C. Between 0 and 20 °C, lipase activity of C. glacialis was higher (40–70% of maximum) than that of the boreal copepods (25–64%), which suggests thermal adaptation of the lipid metabolismin the polar species. Incubating C. glacialis with the diatom Thalassiosira weissflogii showed (i) that enzyme activities increased especially in the alkaline range and (ii) that enzyme patterns, revealed by gel electrophoresis, differed from that of starving individuals, indicating that feeding induced enzyme expression. Such studies, linking abiotic and biotic conditions to enzyme functioning, can help elucidating the capacity of copepods to respond to environmental changes. Article in Journal/Newspaper Calanus glacialis Copepods Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 162 4 66 72 |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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ftawi |
language |
unknown |
description |
In calanoid copepods it is poorly understood how enzymatic activities and patterns are affected by abiotic and biotic factors. Such knowledge, however, is crucial to assess metabolic functioning and performance of organisms in different habitats. Therefore, our study focuses on digestive enzyme activities in relation to temperature,pH and food in the Arctic species Calanus glacialis and in Centropages hamatus and Temora longicornis from the North Sea. Enzyme activities were measured over a range from 0 to 70 °C (lipases/esterases, proteinases)and pH 5 to 9 (proteinases). In all species, relative proteinases activity peaked at 40/50 °C and pH 6; relative lipases/esterases activity peaked at 30 °C. Between 0 and 20 °C, lipase activity of C. glacialis was higher (40–70% of maximum) than that of the boreal copepods (25–64%), which suggests thermal adaptation of the lipid metabolismin the polar species. Incubating C. glacialis with the diatom Thalassiosira weissflogii showed (i) that enzyme activities increased especially in the alkaline range and (ii) that enzyme patterns, revealed by gel electrophoresis, differed from that of starving individuals, indicating that feeding induced enzyme expression. Such studies, linking abiotic and biotic conditions to enzyme functioning, can help elucidating the capacity of copepods to respond to environmental changes. |
format |
Article in Journal/Newspaper |
author |
Freese, Daniela Kreibich, Tobias Niehoff, Barbara |
spellingShingle |
Freese, Daniela Kreibich, Tobias Niehoff, Barbara Characteristics of digestive enzymes of calanoid copepod species from different latitudes in relation to temperature, pH and food |
author_facet |
Freese, Daniela Kreibich, Tobias Niehoff, Barbara |
author_sort |
Freese, Daniela |
title |
Characteristics of digestive enzymes of calanoid copepod species from different latitudes in relation to temperature, pH and food |
title_short |
Characteristics of digestive enzymes of calanoid copepod species from different latitudes in relation to temperature, pH and food |
title_full |
Characteristics of digestive enzymes of calanoid copepod species from different latitudes in relation to temperature, pH and food |
title_fullStr |
Characteristics of digestive enzymes of calanoid copepod species from different latitudes in relation to temperature, pH and food |
title_full_unstemmed |
Characteristics of digestive enzymes of calanoid copepod species from different latitudes in relation to temperature, pH and food |
title_sort |
characteristics of digestive enzymes of calanoid copepod species from different latitudes in relation to temperature, ph and food |
publishDate |
2012 |
url |
https://epic.awi.de/id/eprint/31896/ https://hdl.handle.net/10013/epic.40641 |
genre |
Calanus glacialis Copepods |
genre_facet |
Calanus glacialis Copepods |
op_source |
EPIC3Comparative Biochemistry and Physiology, Part B, 162(4), pp. 66-72 |
op_relation |
Freese, D. , Kreibich, T. and Niehoff, B. (2012) Characteristics of digestive enzymes of calanoid copepod species from different latitudes in relation to temperature, pH and food , Comparative Biochemistry and Physiology, Part B, 162 (4), pp. 66-72 . doi:10.1016/j.cbpb.2012.04.007 <https://doi.org/10.1016/j.cbpb.2012.04.007> , hdl:10013/epic.40641 |
op_doi |
https://doi.org/10.1016/j.cbpb.2012.04.007 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
162 |
container_issue |
4 |
container_start_page |
66 |
op_container_end_page |
72 |
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1810437885587881984 |