Transcriptomic responses of Emiliania huxleyi to Ocean Acidification
To investigate effects of ocean acidification (OA) and their energy-dependent modulation in Emiliania huxleyi, diploid cells (RCC 1216) were acclimated to present and future CO2 partial pressures (pCO2; 380 vs. 1000 µatm) under low and high light (50 vs. 300 µmol photons m-2 s-1). Microarray-based t...
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ftawi:oai:epic.awi.de:31564 2023-05-15T17:50:27+02:00 Transcriptomic responses of Emiliania huxleyi to Ocean Acidification Rokitta, Sebastian D. John, Uwe Rost, Bjoern 2012-09-26 application/pdf https://epic.awi.de/id/eprint/31564/ https://epic.awi.de/id/eprint/31564/1/Rokitta_Ehux_OA_transcriptomics.pdf https://hdl.handle.net/10013/epic.40356 https://hdl.handle.net/10013/epic.40356.d001 unknown https://epic.awi.de/id/eprint/31564/1/Rokitta_Ehux_OA_transcriptomics.pdf https://hdl.handle.net/10013/epic.40356.d001 Rokitta, S. D. orcid:0000-0002-7540-9033 , John, U. orcid:0000-0002-1297-4086 and Rost, B. orcid:0000-0001-5452-5505 (2012) Transcriptomic responses of Emiliania huxleyi to Ocean Acidification , Third Symposium on the Ocean in a High-CO2 World, 24 September 2012 - 27 September 2012 . hdl:10013/epic.40356 EPIC3Third Symposium on the Ocean in a High-CO2 World, 2012-09-24-2012-09-27 Conference notRev 2012 ftawi 2021-12-24T15:38:06Z To investigate effects of ocean acidification (OA) and their energy-dependent modulation in Emiliania huxleyi, diploid cells (RCC 1216) were acclimated to present and future CO2 partial pressures (pCO2; 380 vs. 1000 µatm) under low and high light (50 vs. 300 µmol photons m-2 s-1). Microarray-based transcriptome profiling was used to screen for cellular processes that underlie the physiological responses observed earlier (Rokitta & Rost 2012). OA was shown to influence fluxes of organic metabolites within and across compartments, and their partitioning between oxidative (e.g. glycolysis) and reductive pathways (e.g. pentose phosphate pathway), which is the likely cause for increased POC production. Furthermore, altered signal-transduction (e.g. phosphatidylinositolphosphate- and sphingosine-based signals) and membrane-potentials (e.g. by altered active ion transport) seem to be a major cause of impaired calcification under OA. While OA influenced signal-transduction and ion homeostasis independent of the light level, the effects of OA on the carbon metabolism were prominently modulated by energy availability. This interdependence of carbon metabolism and light physiology likely derives from their reliance on the redox equilibria of NAD+ and NADP+, which are cellular sensors for energy state and stress level. Due to the fundamental role of the affected processes, responses to OA are likely to occur similarly in other marine protists. Conference Object Ocean acidification Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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To investigate effects of ocean acidification (OA) and their energy-dependent modulation in Emiliania huxleyi, diploid cells (RCC 1216) were acclimated to present and future CO2 partial pressures (pCO2; 380 vs. 1000 µatm) under low and high light (50 vs. 300 µmol photons m-2 s-1). Microarray-based transcriptome profiling was used to screen for cellular processes that underlie the physiological responses observed earlier (Rokitta & Rost 2012). OA was shown to influence fluxes of organic metabolites within and across compartments, and their partitioning between oxidative (e.g. glycolysis) and reductive pathways (e.g. pentose phosphate pathway), which is the likely cause for increased POC production. Furthermore, altered signal-transduction (e.g. phosphatidylinositolphosphate- and sphingosine-based signals) and membrane-potentials (e.g. by altered active ion transport) seem to be a major cause of impaired calcification under OA. While OA influenced signal-transduction and ion homeostasis independent of the light level, the effects of OA on the carbon metabolism were prominently modulated by energy availability. This interdependence of carbon metabolism and light physiology likely derives from their reliance on the redox equilibria of NAD+ and NADP+, which are cellular sensors for energy state and stress level. Due to the fundamental role of the affected processes, responses to OA are likely to occur similarly in other marine protists. |
format |
Conference Object |
author |
Rokitta, Sebastian D. John, Uwe Rost, Bjoern |
spellingShingle |
Rokitta, Sebastian D. John, Uwe Rost, Bjoern Transcriptomic responses of Emiliania huxleyi to Ocean Acidification |
author_facet |
Rokitta, Sebastian D. John, Uwe Rost, Bjoern |
author_sort |
Rokitta, Sebastian D. |
title |
Transcriptomic responses of Emiliania huxleyi to Ocean Acidification |
title_short |
Transcriptomic responses of Emiliania huxleyi to Ocean Acidification |
title_full |
Transcriptomic responses of Emiliania huxleyi to Ocean Acidification |
title_fullStr |
Transcriptomic responses of Emiliania huxleyi to Ocean Acidification |
title_full_unstemmed |
Transcriptomic responses of Emiliania huxleyi to Ocean Acidification |
title_sort |
transcriptomic responses of emiliania huxleyi to ocean acidification |
publishDate |
2012 |
url |
https://epic.awi.de/id/eprint/31564/ https://epic.awi.de/id/eprint/31564/1/Rokitta_Ehux_OA_transcriptomics.pdf https://hdl.handle.net/10013/epic.40356 https://hdl.handle.net/10013/epic.40356.d001 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
EPIC3Third Symposium on the Ocean in a High-CO2 World, 2012-09-24-2012-09-27 |
op_relation |
https://epic.awi.de/id/eprint/31564/1/Rokitta_Ehux_OA_transcriptomics.pdf https://hdl.handle.net/10013/epic.40356.d001 Rokitta, S. D. orcid:0000-0002-7540-9033 , John, U. orcid:0000-0002-1297-4086 and Rost, B. orcid:0000-0001-5452-5505 (2012) Transcriptomic responses of Emiliania huxleyi to Ocean Acidification , Third Symposium on the Ocean in a High-CO2 World, 24 September 2012 - 27 September 2012 . hdl:10013/epic.40356 |
_version_ |
1766157212363784192 |