Molecular phylogeny and toxin profiles of Alexandrium tamarense (Lebour) Balech (Dinophyceae) from the west coast of Greenland
Detection of paralytic shellfish poisoning (PSP) toxins in scallops from the west coast of Greenland exceeding the 800 μg toxin/kg shellfish limit led to an investigation with the aim of finding the responsible organism(s). Three strains of Alexandrium Halim were established from single cell isolati...
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Online Access: | https://epic.awi.de/id/eprint/31251/ https://epic.awi.de/id/eprint/31251/1/HARALG-D-12-00041R1.pdf https://hdl.handle.net/10013/epic.40094 https://hdl.handle.net/10013/epic.40094.d001 |
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ftawi:oai:epic.awi.de:31251 2024-09-15T18:09:10+00:00 Molecular phylogeny and toxin profiles of Alexandrium tamarense (Lebour) Balech (Dinophyceae) from the west coast of Greenland Baggesen, Claus Moestrup, Øjvind Daubjerg, Niels Krock, Bernd Cembella, Allan Madsen, Sine 2012-06-26 application/pdf https://epic.awi.de/id/eprint/31251/ https://epic.awi.de/id/eprint/31251/1/HARALG-D-12-00041R1.pdf https://hdl.handle.net/10013/epic.40094 https://hdl.handle.net/10013/epic.40094.d001 unknown ELSEVIER SCIENCE BV https://epic.awi.de/id/eprint/31251/1/HARALG-D-12-00041R1.pdf https://hdl.handle.net/10013/epic.40094.d001 Baggesen, C. , Moestrup, Ø. , Daubjerg, N. , Krock, B. orcid:0000-0003-4022-9101 , Cembella, A. and Madsen, S. (2012) Molecular phylogeny and toxin profiles of Alexandrium tamarense (Lebour) Balech (Dinophyceae) from the west coast of Greenland , Harmful Algae, 19 , pp. 108-116 . doi:10.1016/j.hal.2012.06.005 <https://doi.org/10.1016/j.hal.2012.06.005> , hdl:10013/epic.40094 EPIC3Harmful Algae, ELSEVIER SCIENCE BV, 19, pp. 108-116, ISSN: 1568-9883 Article isiRev 2012 ftawi https://doi.org/10.1016/j.hal.2012.06.005 2024-06-24T04:05:07Z Detection of paralytic shellfish poisoning (PSP) toxins in scallops from the west coast of Greenland exceeding the 800 μg toxin/kg shellfish limit led to an investigation with the aim of finding the responsible organism(s). Three strains of Alexandrium Halim were established from single cell isolations. Morphological identification of the strains and determination of their position within the genus by LSU rDNA sequences was carried out. Light microscopy revealed that the three strains was of the Alexandrium tamarense morphotype, and bayesian and neighbor-joining analyses of the LSU rDNA sequences placed them within Group I of the A. tamarense species complex. The toxicity and toxin profiles of the strains were measured by liquid chromatography fluorescence detection (LC-FD) and their identity was confirmed by liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS). The three strains all turned out to be toxic and all produced large proportions (>60% total mol) of gonyautoxins 1 and 4 (GTX1/GTX4). This is the first record of saxitoxin producers from western Greenland. The toxin profiles were atypical for A. tamarense in their absence of N-sulfocarbanoyl C1/C2 or B1/B2 toxins. Rather the high molar percentage of GTX1/GTX4, the lesser amounts of only carbamoyl toxins and the absence of decarbamoyl derivatives are more characteristic features of A. minutum strains. This may indicate that the genetically determined toxin profiles in Alexandrium species are more complex than previously appreciated. Article in Journal/Newspaper Greenland Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Harmful Algae 19 108 116 |
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Open Polar |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
Detection of paralytic shellfish poisoning (PSP) toxins in scallops from the west coast of Greenland exceeding the 800 μg toxin/kg shellfish limit led to an investigation with the aim of finding the responsible organism(s). Three strains of Alexandrium Halim were established from single cell isolations. Morphological identification of the strains and determination of their position within the genus by LSU rDNA sequences was carried out. Light microscopy revealed that the three strains was of the Alexandrium tamarense morphotype, and bayesian and neighbor-joining analyses of the LSU rDNA sequences placed them within Group I of the A. tamarense species complex. The toxicity and toxin profiles of the strains were measured by liquid chromatography fluorescence detection (LC-FD) and their identity was confirmed by liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS). The three strains all turned out to be toxic and all produced large proportions (>60% total mol) of gonyautoxins 1 and 4 (GTX1/GTX4). This is the first record of saxitoxin producers from western Greenland. The toxin profiles were atypical for A. tamarense in their absence of N-sulfocarbanoyl C1/C2 or B1/B2 toxins. Rather the high molar percentage of GTX1/GTX4, the lesser amounts of only carbamoyl toxins and the absence of decarbamoyl derivatives are more characteristic features of A. minutum strains. This may indicate that the genetically determined toxin profiles in Alexandrium species are more complex than previously appreciated. |
format |
Article in Journal/Newspaper |
author |
Baggesen, Claus Moestrup, Øjvind Daubjerg, Niels Krock, Bernd Cembella, Allan Madsen, Sine |
spellingShingle |
Baggesen, Claus Moestrup, Øjvind Daubjerg, Niels Krock, Bernd Cembella, Allan Madsen, Sine Molecular phylogeny and toxin profiles of Alexandrium tamarense (Lebour) Balech (Dinophyceae) from the west coast of Greenland |
author_facet |
Baggesen, Claus Moestrup, Øjvind Daubjerg, Niels Krock, Bernd Cembella, Allan Madsen, Sine |
author_sort |
Baggesen, Claus |
title |
Molecular phylogeny and toxin profiles of Alexandrium tamarense (Lebour) Balech (Dinophyceae) from the west coast of Greenland |
title_short |
Molecular phylogeny and toxin profiles of Alexandrium tamarense (Lebour) Balech (Dinophyceae) from the west coast of Greenland |
title_full |
Molecular phylogeny and toxin profiles of Alexandrium tamarense (Lebour) Balech (Dinophyceae) from the west coast of Greenland |
title_fullStr |
Molecular phylogeny and toxin profiles of Alexandrium tamarense (Lebour) Balech (Dinophyceae) from the west coast of Greenland |
title_full_unstemmed |
Molecular phylogeny and toxin profiles of Alexandrium tamarense (Lebour) Balech (Dinophyceae) from the west coast of Greenland |
title_sort |
molecular phylogeny and toxin profiles of alexandrium tamarense (lebour) balech (dinophyceae) from the west coast of greenland |
publisher |
ELSEVIER SCIENCE BV |
publishDate |
2012 |
url |
https://epic.awi.de/id/eprint/31251/ https://epic.awi.de/id/eprint/31251/1/HARALG-D-12-00041R1.pdf https://hdl.handle.net/10013/epic.40094 https://hdl.handle.net/10013/epic.40094.d001 |
genre |
Greenland |
genre_facet |
Greenland |
op_source |
EPIC3Harmful Algae, ELSEVIER SCIENCE BV, 19, pp. 108-116, ISSN: 1568-9883 |
op_relation |
https://epic.awi.de/id/eprint/31251/1/HARALG-D-12-00041R1.pdf https://hdl.handle.net/10013/epic.40094.d001 Baggesen, C. , Moestrup, Ø. , Daubjerg, N. , Krock, B. orcid:0000-0003-4022-9101 , Cembella, A. and Madsen, S. (2012) Molecular phylogeny and toxin profiles of Alexandrium tamarense (Lebour) Balech (Dinophyceae) from the west coast of Greenland , Harmful Algae, 19 , pp. 108-116 . doi:10.1016/j.hal.2012.06.005 <https://doi.org/10.1016/j.hal.2012.06.005> , hdl:10013/epic.40094 |
op_doi |
https://doi.org/10.1016/j.hal.2012.06.005 |
container_title |
Harmful Algae |
container_volume |
19 |
container_start_page |
108 |
op_container_end_page |
116 |
_version_ |
1810446580610760704 |