Dominance of oligotrophic bacteria in surface waters above Gunnerus and Astrid Ridge, Antarctic Ocean
From enrichment cultures in dialysis chambers held in natural seawater tanks, 104 strains were isolated and kept in culture. All strains proved to be Gram-negative and psychrotrophic, having optimum growth temperatures of between 20 and 24 °C. Maximal growth temperatures were 30 to 37 °C, or even hi...
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ftawi:oai:epic.awi.de:3068 2023-09-05T13:13:23+02:00 Dominance of oligotrophic bacteria in surface waters above Gunnerus and Astrid Ridge, Antarctic Ocean Tan, T. L. Joiris, C. R. Glansdorff, N. Rüger, H.-J. 1999 https://epic.awi.de/id/eprint/3068/ https://hdl.handle.net/10013/epic.13652 unknown Tan, T. L. , Joiris, C. R. , Glansdorff, N. and Rüger, H. J. (1999) Dominance of oligotrophic bacteria in surface waters above Gunnerus and Astrid Ridge, Antarctic Ocean , Archiv für Hydrobiologie, Special Issues Advances in Limnology, 54 , pp. 237-253 . hdl:10013/epic.13652 EPIC3Archiv für Hydrobiologie, Special Issues Advances in Limnology, 54, pp. 237-253 Article isiRev 1999 ftawi 2023-08-22T19:43:47Z From enrichment cultures in dialysis chambers held in natural seawater tanks, 104 strains were isolated and kept in culture. All strains proved to be Gram-negative and psychrotrophic, having optimum growth temperatures of between 20 and 24 °C. Maximal growth temperatures were 30 to 37 °C, or even higher. With 55 isolates, substrate utilizations in Biolog MicroPlates were determined, and the obtained metabolic fingerprints used for clustering. Five groups could be distinguished at the 80% similarity level. Fifteen strains belonged to cluster 1, seven strains to cluster 2, and each of the clusters 3 and 4 contained nine strains. Cluster 5 can be divided into subcluster 5a and 5b, with 6 strains showing a few substrates metabolized, and 9 strains without any reactions, or weak reactions for one or two substrates, respectively. Each cluster could be characterized by specific metabolic fingerprints. Strains from cluster 1 metabolized N-acetyl-D-glucosamine, alpha-hydroxybutyric acid and gamma-hydroxybutyric acid, strains from cluster 2 citric acid, formic acid, thymidine and putrescine, strains from cluster 3 glycyl-L-aspartic acid, glycyl-L-glutamic acid, L-threonine and inosine, whereas strains from cluster 4 metabolized alpha-cyclodextrin and N-acetyl-D-galactosamine, typically. Methylamine was not utilized by the isolates, but strains from cluster 1, 2 and 3 could grow on basal seawater agar. Morphological characteristics and photomicrographs of the oligotrophic strains are presented. Due to their typical morphologies and ampicillin resistence, the nine strains from cluster 3 can be regarded as new species of the genus Planctomyces. These bacteria have not been cultivated before. Article in Journal/Newspaper Antarc* Antarctic Antarctic Ocean Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Antarctic Antarctic Ocean Astrid Ridge ENVELOPE(12.000,12.000,-68.000,-68.000) |
institution |
Open Polar |
collection |
Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
From enrichment cultures in dialysis chambers held in natural seawater tanks, 104 strains were isolated and kept in culture. All strains proved to be Gram-negative and psychrotrophic, having optimum growth temperatures of between 20 and 24 °C. Maximal growth temperatures were 30 to 37 °C, or even higher. With 55 isolates, substrate utilizations in Biolog MicroPlates were determined, and the obtained metabolic fingerprints used for clustering. Five groups could be distinguished at the 80% similarity level. Fifteen strains belonged to cluster 1, seven strains to cluster 2, and each of the clusters 3 and 4 contained nine strains. Cluster 5 can be divided into subcluster 5a and 5b, with 6 strains showing a few substrates metabolized, and 9 strains without any reactions, or weak reactions for one or two substrates, respectively. Each cluster could be characterized by specific metabolic fingerprints. Strains from cluster 1 metabolized N-acetyl-D-glucosamine, alpha-hydroxybutyric acid and gamma-hydroxybutyric acid, strains from cluster 2 citric acid, formic acid, thymidine and putrescine, strains from cluster 3 glycyl-L-aspartic acid, glycyl-L-glutamic acid, L-threonine and inosine, whereas strains from cluster 4 metabolized alpha-cyclodextrin and N-acetyl-D-galactosamine, typically. Methylamine was not utilized by the isolates, but strains from cluster 1, 2 and 3 could grow on basal seawater agar. Morphological characteristics and photomicrographs of the oligotrophic strains are presented. Due to their typical morphologies and ampicillin resistence, the nine strains from cluster 3 can be regarded as new species of the genus Planctomyces. These bacteria have not been cultivated before. |
format |
Article in Journal/Newspaper |
author |
Tan, T. L. Joiris, C. R. Glansdorff, N. Rüger, H.-J. |
spellingShingle |
Tan, T. L. Joiris, C. R. Glansdorff, N. Rüger, H.-J. Dominance of oligotrophic bacteria in surface waters above Gunnerus and Astrid Ridge, Antarctic Ocean |
author_facet |
Tan, T. L. Joiris, C. R. Glansdorff, N. Rüger, H.-J. |
author_sort |
Tan, T. L. |
title |
Dominance of oligotrophic bacteria in surface waters above Gunnerus and Astrid Ridge, Antarctic Ocean |
title_short |
Dominance of oligotrophic bacteria in surface waters above Gunnerus and Astrid Ridge, Antarctic Ocean |
title_full |
Dominance of oligotrophic bacteria in surface waters above Gunnerus and Astrid Ridge, Antarctic Ocean |
title_fullStr |
Dominance of oligotrophic bacteria in surface waters above Gunnerus and Astrid Ridge, Antarctic Ocean |
title_full_unstemmed |
Dominance of oligotrophic bacteria in surface waters above Gunnerus and Astrid Ridge, Antarctic Ocean |
title_sort |
dominance of oligotrophic bacteria in surface waters above gunnerus and astrid ridge, antarctic ocean |
publishDate |
1999 |
url |
https://epic.awi.de/id/eprint/3068/ https://hdl.handle.net/10013/epic.13652 |
long_lat |
ENVELOPE(12.000,12.000,-68.000,-68.000) |
geographic |
Antarctic Antarctic Ocean Astrid Ridge |
geographic_facet |
Antarctic Antarctic Ocean Astrid Ridge |
genre |
Antarc* Antarctic Antarctic Ocean |
genre_facet |
Antarc* Antarctic Antarctic Ocean |
op_source |
EPIC3Archiv für Hydrobiologie, Special Issues Advances in Limnology, 54, pp. 237-253 |
op_relation |
Tan, T. L. , Joiris, C. R. , Glansdorff, N. and Rüger, H. J. (1999) Dominance of oligotrophic bacteria in surface waters above Gunnerus and Astrid Ridge, Antarctic Ocean , Archiv für Hydrobiologie, Special Issues Advances in Limnology, 54 , pp. 237-253 . hdl:10013/epic.13652 |
_version_ |
1776204679644971008 |