Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel: implications for shell formation and energy supply
Marine organisms have to cope with increasing CO2 partial pressures and decreasing pH in the oceans. We elucidated the impacts of an 8-week acclimation period to four seawater pCO2 treatments (39, 113, 243 and 405 Pa/385, 1,120, 2,400 and 4,000 µatm) on mantle gene expression patterns in the blue mu...
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Online Access: | https://epic.awi.de/id/eprint/30660/ https://hdl.handle.net/10013/epic.39590 |
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ftawi:oai:epic.awi.de:30660 2024-09-15T18:28:16+00:00 Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel: implications for shell formation and energy supply Hüning, Anne K. Melzner, Frank Thomsen, Jörn Gutowska, Magdalena A. Krämer, Lars Frickenhaus, Stephan Rosenstiel, Philip Pörtner, Hans-Otto Philipp, Eva E. R. Lucassen, Magnus 2012 https://epic.awi.de/id/eprint/30660/ https://hdl.handle.net/10013/epic.39590 unknown Hüning, A. K. , Melzner, F. , Thomsen, J. , Gutowska, M. A. , Krämer, L. , Frickenhaus, S. orcid:0000-0002-0356-9791 , Rosenstiel, P. , Pörtner, H. O. orcid:0000-0001-6535-6575 , Philipp, E. E. R. and Lucassen, M. orcid:0000-0003-4276-4781 (2012) Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel: implications for shell formation and energy supply , Marine Biology . doi:10.1007/s00227-012-1930-9 <https://doi.org/10.1007/s00227-012-1930-9> , hdl:10013/epic.39590 EPIC3Marine Biology Article isiRev 2012 ftawi https://doi.org/10.1007/s00227-012-1930-9 2024-06-24T04:05:07Z Marine organisms have to cope with increasing CO2 partial pressures and decreasing pH in the oceans. We elucidated the impacts of an 8-week acclimation period to four seawater pCO2 treatments (39, 113, 243 and 405 Pa/385, 1,120, 2,400 and 4,000 µatm) on mantle gene expression patterns in the blue mussel Mytilus edulis from the Baltic Sea. Based on the M. edulis mantle tissue transcriptome, the expression of several genes involved in metabolism, calcification and stress responses was assessed in the outer (marginal and pallial zone) and the inner mantle tissues (central zone) using quantitative real-time PCR. The expression of genes involved in energy and protein metabolism (F-ATPase, hexokinase and elongation factor alpha) was strongly affected by acclimation to moderately elevated CO2 partial pressures. Expression of a chitinase, potentially important for the calcification process, was strongly depressed (maximum ninefold), correlating with a linear decrease in shell growth observed in the experimental animals. Interestingly, shell matrix protein candidate genes were less affected by CO2 in both tissues. A compensatory process toward enhanced shell protection is indicated by a massive increase in the expression of tyrosinase, a gene involved in periostracum formation (maximum 220-fold). Using correlation matrices and a force-directed layout network graph, we were able to uncover possible underlying regulatory networks and the connections between different pathways, thereby providing a molecular basis of observed changes in animal physiology in response to ocean acidification. Article in Journal/Newspaper Ocean acidification Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Marine Biology 160 8 1845 1861 |
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Open Polar |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
Marine organisms have to cope with increasing CO2 partial pressures and decreasing pH in the oceans. We elucidated the impacts of an 8-week acclimation period to four seawater pCO2 treatments (39, 113, 243 and 405 Pa/385, 1,120, 2,400 and 4,000 µatm) on mantle gene expression patterns in the blue mussel Mytilus edulis from the Baltic Sea. Based on the M. edulis mantle tissue transcriptome, the expression of several genes involved in metabolism, calcification and stress responses was assessed in the outer (marginal and pallial zone) and the inner mantle tissues (central zone) using quantitative real-time PCR. The expression of genes involved in energy and protein metabolism (F-ATPase, hexokinase and elongation factor alpha) was strongly affected by acclimation to moderately elevated CO2 partial pressures. Expression of a chitinase, potentially important for the calcification process, was strongly depressed (maximum ninefold), correlating with a linear decrease in shell growth observed in the experimental animals. Interestingly, shell matrix protein candidate genes were less affected by CO2 in both tissues. A compensatory process toward enhanced shell protection is indicated by a massive increase in the expression of tyrosinase, a gene involved in periostracum formation (maximum 220-fold). Using correlation matrices and a force-directed layout network graph, we were able to uncover possible underlying regulatory networks and the connections between different pathways, thereby providing a molecular basis of observed changes in animal physiology in response to ocean acidification. |
format |
Article in Journal/Newspaper |
author |
Hüning, Anne K. Melzner, Frank Thomsen, Jörn Gutowska, Magdalena A. Krämer, Lars Frickenhaus, Stephan Rosenstiel, Philip Pörtner, Hans-Otto Philipp, Eva E. R. Lucassen, Magnus |
spellingShingle |
Hüning, Anne K. Melzner, Frank Thomsen, Jörn Gutowska, Magdalena A. Krämer, Lars Frickenhaus, Stephan Rosenstiel, Philip Pörtner, Hans-Otto Philipp, Eva E. R. Lucassen, Magnus Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel: implications for shell formation and energy supply |
author_facet |
Hüning, Anne K. Melzner, Frank Thomsen, Jörn Gutowska, Magdalena A. Krämer, Lars Frickenhaus, Stephan Rosenstiel, Philip Pörtner, Hans-Otto Philipp, Eva E. R. Lucassen, Magnus |
author_sort |
Hüning, Anne K. |
title |
Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel: implications for shell formation and energy supply |
title_short |
Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel: implications for shell formation and energy supply |
title_full |
Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel: implications for shell formation and energy supply |
title_fullStr |
Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel: implications for shell formation and energy supply |
title_full_unstemmed |
Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel: implications for shell formation and energy supply |
title_sort |
impacts of seawater acidification on mantle gene expression patterns of the baltic sea blue mussel: implications for shell formation and energy supply |
publishDate |
2012 |
url |
https://epic.awi.de/id/eprint/30660/ https://hdl.handle.net/10013/epic.39590 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
EPIC3Marine Biology |
op_relation |
Hüning, A. K. , Melzner, F. , Thomsen, J. , Gutowska, M. A. , Krämer, L. , Frickenhaus, S. orcid:0000-0002-0356-9791 , Rosenstiel, P. , Pörtner, H. O. orcid:0000-0001-6535-6575 , Philipp, E. E. R. and Lucassen, M. orcid:0000-0003-4276-4781 (2012) Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel: implications for shell formation and energy supply , Marine Biology . doi:10.1007/s00227-012-1930-9 <https://doi.org/10.1007/s00227-012-1930-9> , hdl:10013/epic.39590 |
op_doi |
https://doi.org/10.1007/s00227-012-1930-9 |
container_title |
Marine Biology |
container_volume |
160 |
container_issue |
8 |
container_start_page |
1845 |
op_container_end_page |
1861 |
_version_ |
1810469617603182592 |