Effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi
The effects of ocean acidification on the life-cycle stages of the coccolithophore Emiliania huxleyi and their modulation by light were examined. Calcifying diploid and noncalcifying haploid cells (Roscoff culture collection strains 1216 and 1217) were acclimated to present-day and elevated CO2 part...
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Online Access: | https://epic.awi.de/id/eprint/26059/ https://epic.awi.de/id/eprint/26059/1/0607.pdf http://www.aslo.org/lo/toc/vol_57/issue_2/0607.pdf https://hdl.handle.net/10013/epic.39064 https://hdl.handle.net/10013/epic.39064.d001 |
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ftawi:oai:epic.awi.de:26059 2024-09-15T18:27:49+00:00 Effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi Rokitta, Sebastian D. Rost, Björn 2012-03-28 application/pdf https://epic.awi.de/id/eprint/26059/ https://epic.awi.de/id/eprint/26059/1/0607.pdf http://www.aslo.org/lo/toc/vol_57/issue_2/0607.pdf https://hdl.handle.net/10013/epic.39064 https://hdl.handle.net/10013/epic.39064.d001 unknown ASLO https://epic.awi.de/id/eprint/26059/1/0607.pdf https://hdl.handle.net/10013/epic.39064.d001 Rokitta, S. D. orcid:0000-0002-7540-9033 and Rost, B. orcid:0000-0001-5452-5505 (2012) Effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi , Limnology & Oceanography, 57 (2), pp. 607-618 . doi:10.4319/lo.2012.57.2.0607 <https://doi.org/10.4319/lo.2012.57.2.0607> , hdl:10013/epic.39064 EPIC3Limnology & Oceanography, ASLO, 57(2), pp. 607-618, ISSN: 1939-5590 Article isiRev 2012 ftawi 2024-06-24T04:03:41Z The effects of ocean acidification on the life-cycle stages of the coccolithophore Emiliania huxleyi and their modulation by light were examined. Calcifying diploid and noncalcifying haploid cells (Roscoff culture collection strains 1216 and 1217) were acclimated to present-day and elevated CO2 partial pressures (PCO2; 38.5 vs. 101.3 Pa, i.e., 380 vs. 1000 µatm) under low and high light (50 vs. 300 µmol photons m-2 s-1). Growth rates as well as cellular quotas and production rates of C and N were measured. Sources of inorganic C for biomass buildup were determined using a 14C disequilibrium assay. Photosynthetic O2 evolution was measured as a function of dissolved inorganic C and light by means of membrane-inlet mass spectrometry. The diploid stage responded to elevated PCO2 by shunting resources from the production of particulate inorganic C toward organic C yet keeping the production of total particulate C constant. As the effect of ocean acidification was stronger under low light, the diploid stage might be less affected by increased acidity when energy availability is high. The haploid stage maintained elemental composition and production rates under elevated PCO2. Although both life-cycle stages involve different ways of dealing with elevated PCO2, the responses were generally modulated by energy availability, being typically most pronounced under low light. Additionally, PCO2 responses resembled those induced by high irradiances, indicating that ocean acidification affects the interplay between energy-generating processes (photosynthetic light reactions) and processes competing for energy (biomass buildup and calcification). A conceptual model is put forward explaining why the magnitude of single responses is determined by energy availability. Article in Journal/Newspaper Ocean acidification Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Limnology and Oceanography 57 2 607 618 |
institution |
Open Polar |
collection |
Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
The effects of ocean acidification on the life-cycle stages of the coccolithophore Emiliania huxleyi and their modulation by light were examined. Calcifying diploid and noncalcifying haploid cells (Roscoff culture collection strains 1216 and 1217) were acclimated to present-day and elevated CO2 partial pressures (PCO2; 38.5 vs. 101.3 Pa, i.e., 380 vs. 1000 µatm) under low and high light (50 vs. 300 µmol photons m-2 s-1). Growth rates as well as cellular quotas and production rates of C and N were measured. Sources of inorganic C for biomass buildup were determined using a 14C disequilibrium assay. Photosynthetic O2 evolution was measured as a function of dissolved inorganic C and light by means of membrane-inlet mass spectrometry. The diploid stage responded to elevated PCO2 by shunting resources from the production of particulate inorganic C toward organic C yet keeping the production of total particulate C constant. As the effect of ocean acidification was stronger under low light, the diploid stage might be less affected by increased acidity when energy availability is high. The haploid stage maintained elemental composition and production rates under elevated PCO2. Although both life-cycle stages involve different ways of dealing with elevated PCO2, the responses were generally modulated by energy availability, being typically most pronounced under low light. Additionally, PCO2 responses resembled those induced by high irradiances, indicating that ocean acidification affects the interplay between energy-generating processes (photosynthetic light reactions) and processes competing for energy (biomass buildup and calcification). A conceptual model is put forward explaining why the magnitude of single responses is determined by energy availability. |
format |
Article in Journal/Newspaper |
author |
Rokitta, Sebastian D. Rost, Björn |
spellingShingle |
Rokitta, Sebastian D. Rost, Björn Effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi |
author_facet |
Rokitta, Sebastian D. Rost, Björn |
author_sort |
Rokitta, Sebastian D. |
title |
Effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi |
title_short |
Effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi |
title_full |
Effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi |
title_fullStr |
Effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi |
title_full_unstemmed |
Effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi |
title_sort |
effects of co2 and their modulation by light in the life-cycle stages of the coccolithophore emiliania huxleyi |
publisher |
ASLO |
publishDate |
2012 |
url |
https://epic.awi.de/id/eprint/26059/ https://epic.awi.de/id/eprint/26059/1/0607.pdf http://www.aslo.org/lo/toc/vol_57/issue_2/0607.pdf https://hdl.handle.net/10013/epic.39064 https://hdl.handle.net/10013/epic.39064.d001 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
EPIC3Limnology & Oceanography, ASLO, 57(2), pp. 607-618, ISSN: 1939-5590 |
op_relation |
https://epic.awi.de/id/eprint/26059/1/0607.pdf https://hdl.handle.net/10013/epic.39064.d001 Rokitta, S. D. orcid:0000-0002-7540-9033 and Rost, B. orcid:0000-0001-5452-5505 (2012) Effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi , Limnology & Oceanography, 57 (2), pp. 607-618 . doi:10.4319/lo.2012.57.2.0607 <https://doi.org/10.4319/lo.2012.57.2.0607> , hdl:10013/epic.39064 |
container_title |
Limnology and Oceanography |
container_volume |
57 |
container_issue |
2 |
container_start_page |
607 |
op_container_end_page |
618 |
_version_ |
1810469090127511552 |