Microbial driven methane dynamics in the Siberian Arctic during glacial-interglacial climate changes
The currently observed climate change due to global warming is expected to have a strong impact especially on the Arctic permafrost environments. The thawing of permafrost is suggested to be associated with a massive release of greenhouse gases, in particular methane. For the understanding how the s...
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ftawi:oai:epic.awi.de:23177 2023-05-15T14:24:51+02:00 Microbial driven methane dynamics in the Siberian Arctic during glacial-interglacial climate changes Griess, Juliane Wagner, Dirk Mangelsdorf, K. 2010 https://epic.awi.de/id/eprint/23177/ https://hdl.handle.net/10013/epic.35947 unknown Griess, J. , Wagner, D. and Mangelsdorf, K. (2010) Microbial driven methane dynamics in the Siberian Arctic during glacial-interglacial climate changes , VAAM Annual Conference, Hannover, Germany, March 28 - . hdl:10013/epic.35947 EPIC3VAAM Annual Conference, Hannover, Germany, March 28 -, pp. 31 2010 Conference notRev 2010 ftawi 2021-12-24T15:34:34Z The currently observed climate change due to global warming is expected to have a strong impact especially on the Arctic permafrost environments. The thawing of permafrost is suggested to be associated with a massive release of greenhouse gases, in particular methane. For the understanding how the system will respond to climate changes it is not only important to investigate the current status of carbon turnover but also how the system reacted to climate changes in the past. Therefore quantitative and qualitative analyses of the variations in composition of bacterial and archaeal communities involved in the Siberian methane cycle in holocene and late pleistocene were conducted, using samples of a permafrost core drilled in central Lena-Delta, Siberia, in 2002. Our studies on the reconstruction of the methane cycle in deposits of the permafrost affected soils combined methods of biogeochemistry and molecular geomicrobiology. It was possible to recover lipid biomarker and intact DNA continuously throughout the core. Biomarkers like glycerol dialkyl glycerol tetraethers (GDGTs) were analyzed whereas highest amounts of ether lipids were found in the upper layer and at the bottom of the core. Generally, the results of GDGTs analyzes fit to measured rates of total organic carbon (TOC) and in-situ methane concentration of the deposits. Furthermore these biomarkers were analyzed and distinguished in biomarkers representing signals of paleo-archaeal and paleo-bacterial communities. To complete information on the qualitative composition of microbial communities DNA analyses were driven using archaeal and methanotrophic specific primer combinations, whereas amplicons were subsequently analyzed by DGGE and clone libraries. Fingerprints of archaeal 16 S rRNA gene sequences of the different soil samples show variations within the vertical profile. Conference Object Arctic Arctic Climate change Global warming lena delta permafrost Siberia Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Arctic |
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Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
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ftawi |
language |
unknown |
description |
The currently observed climate change due to global warming is expected to have a strong impact especially on the Arctic permafrost environments. The thawing of permafrost is suggested to be associated with a massive release of greenhouse gases, in particular methane. For the understanding how the system will respond to climate changes it is not only important to investigate the current status of carbon turnover but also how the system reacted to climate changes in the past. Therefore quantitative and qualitative analyses of the variations in composition of bacterial and archaeal communities involved in the Siberian methane cycle in holocene and late pleistocene were conducted, using samples of a permafrost core drilled in central Lena-Delta, Siberia, in 2002. Our studies on the reconstruction of the methane cycle in deposits of the permafrost affected soils combined methods of biogeochemistry and molecular geomicrobiology. It was possible to recover lipid biomarker and intact DNA continuously throughout the core. Biomarkers like glycerol dialkyl glycerol tetraethers (GDGTs) were analyzed whereas highest amounts of ether lipids were found in the upper layer and at the bottom of the core. Generally, the results of GDGTs analyzes fit to measured rates of total organic carbon (TOC) and in-situ methane concentration of the deposits. Furthermore these biomarkers were analyzed and distinguished in biomarkers representing signals of paleo-archaeal and paleo-bacterial communities. To complete information on the qualitative composition of microbial communities DNA analyses were driven using archaeal and methanotrophic specific primer combinations, whereas amplicons were subsequently analyzed by DGGE and clone libraries. Fingerprints of archaeal 16 S rRNA gene sequences of the different soil samples show variations within the vertical profile. |
format |
Conference Object |
author |
Griess, Juliane Wagner, Dirk Mangelsdorf, K. |
spellingShingle |
Griess, Juliane Wagner, Dirk Mangelsdorf, K. Microbial driven methane dynamics in the Siberian Arctic during glacial-interglacial climate changes |
author_facet |
Griess, Juliane Wagner, Dirk Mangelsdorf, K. |
author_sort |
Griess, Juliane |
title |
Microbial driven methane dynamics in the Siberian Arctic during glacial-interglacial climate changes |
title_short |
Microbial driven methane dynamics in the Siberian Arctic during glacial-interglacial climate changes |
title_full |
Microbial driven methane dynamics in the Siberian Arctic during glacial-interglacial climate changes |
title_fullStr |
Microbial driven methane dynamics in the Siberian Arctic during glacial-interglacial climate changes |
title_full_unstemmed |
Microbial driven methane dynamics in the Siberian Arctic during glacial-interglacial climate changes |
title_sort |
microbial driven methane dynamics in the siberian arctic during glacial-interglacial climate changes |
publishDate |
2010 |
url |
https://epic.awi.de/id/eprint/23177/ https://hdl.handle.net/10013/epic.35947 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic Climate change Global warming lena delta permafrost Siberia |
genre_facet |
Arctic Arctic Climate change Global warming lena delta permafrost Siberia |
op_source |
EPIC3VAAM Annual Conference, Hannover, Germany, March 28 -, pp. 31 2010 |
op_relation |
Griess, J. , Wagner, D. and Mangelsdorf, K. (2010) Microbial driven methane dynamics in the Siberian Arctic during glacial-interglacial climate changes , VAAM Annual Conference, Hannover, Germany, March 28 - . hdl:10013/epic.35947 |
_version_ |
1766297301228191744 |