Temperature and light requirements for growth of two diatom species (Bacillariophyceae) isolated from an Arctic macroalga
In the present study, two abundant epiphyticdiatom taxa were isolated from the assimilation hairs ofthe brown macroalga Chordaria flagelliformis collected inthe Arctic Kongsfjorden (Spitsbergen, Norway), establishedas unialgal cultures and their growth rates determinedunder controlled photon fluence...
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ftawi:oai:epic.awi.de:13851 2023-09-05T13:15:06+02:00 Temperature and light requirements for growth of two diatom species (Bacillariophyceae) isolated from an Arctic macroalga Karsten, Ulf 2005 https://epic.awi.de/id/eprint/13851/ https://doi.org/10.1007/s00300-005-0078-1 https://hdl.handle.net/10013/epic.24214 unknown Karsten, U. (2005) Temperature and light requirements for growth of two diatom species (Bacillariophyceae) isolated from an Arctic macroalga , Polar Biology . doi:10.1007/s00300-005-0078-1 <https://doi.org/10.1007/s00300-005-0078-1> , hdl:10013/epic.24214 EPIC3Polar Biology, ISSN: 0722-4060 Article isiRev 2005 ftawi https://doi.org/10.1007/s00300-005-0078-1 2023-08-22T19:50:49Z In the present study, two abundant epiphyticdiatom taxa were isolated from the assimilation hairs ofthe brown macroalga Chordaria flagelliformis collected inthe Arctic Kongsfjorden (Spitsbergen, Norway), establishedas unialgal cultures and their growth rates determinedunder controlled photon fluence rate andtemperature conditions. Using morphological (light andscanning electron microscopy) and SSU rRNA gene databoth isolates (ROS D99 and ROS D125) were identifiedas members of a FragilariaSynedropsis clade. Themolecular data of ROS D99 and ROS D125 were not identical to any other published sequence. While ROSD99 has been identified as Fragilaria barbararum mainlydue to the SEM characteristics, ROS D125 could not bedefinitely identified although morphological data speakfor Fragilaria striatula. Both diatom species showedsimilar growth rates at all temperatures and photon fluencerates tested. They grew well between 0 and 15°Cwithoptimum temperatures of 1214°C, but did not survive 20°C. Therefore, compared to Antarctic diatoms bothtaxa from Kongsfjorden can be characterised as eurythermalorganisms. Increasing photon fluence rates between 2 and 15 µmol m-2 s-1 were accompanied with analmost twofold increase in growth rates, but photon fluencerates >15 µmol m-2 s-1 did not further enhancegrowth pointing to low light requirements. From thesedata optimum, minimum and maximum photon fluencerates and temperatures for growth can be assessed indicatingthat both diatoms are well acclimated to the fluctuatingenvironmental conditions in the Arctic habitat. Article in Journal/Newspaper Antarc* Antarctic Arctic Arctic Kongsfjord* Kongsfjorden Polar Biology Spitsbergen Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) Antarctic Arctic Norway Polar Biology 29 6 476 486 |
institution |
Open Polar |
collection |
Alfred Wegener Institute for Polar- and Marine Research (AWI): ePIC (electronic Publication Information Center) |
op_collection_id |
ftawi |
language |
unknown |
description |
In the present study, two abundant epiphyticdiatom taxa were isolated from the assimilation hairs ofthe brown macroalga Chordaria flagelliformis collected inthe Arctic Kongsfjorden (Spitsbergen, Norway), establishedas unialgal cultures and their growth rates determinedunder controlled photon fluence rate andtemperature conditions. Using morphological (light andscanning electron microscopy) and SSU rRNA gene databoth isolates (ROS D99 and ROS D125) were identifiedas members of a FragilariaSynedropsis clade. Themolecular data of ROS D99 and ROS D125 were not identical to any other published sequence. While ROSD99 has been identified as Fragilaria barbararum mainlydue to the SEM characteristics, ROS D125 could not bedefinitely identified although morphological data speakfor Fragilaria striatula. Both diatom species showedsimilar growth rates at all temperatures and photon fluencerates tested. They grew well between 0 and 15°Cwithoptimum temperatures of 1214°C, but did not survive 20°C. Therefore, compared to Antarctic diatoms bothtaxa from Kongsfjorden can be characterised as eurythermalorganisms. Increasing photon fluence rates between 2 and 15 µmol m-2 s-1 were accompanied with analmost twofold increase in growth rates, but photon fluencerates >15 µmol m-2 s-1 did not further enhancegrowth pointing to low light requirements. From thesedata optimum, minimum and maximum photon fluencerates and temperatures for growth can be assessed indicatingthat both diatoms are well acclimated to the fluctuatingenvironmental conditions in the Arctic habitat. |
format |
Article in Journal/Newspaper |
author |
Karsten, Ulf |
spellingShingle |
Karsten, Ulf Temperature and light requirements for growth of two diatom species (Bacillariophyceae) isolated from an Arctic macroalga |
author_facet |
Karsten, Ulf |
author_sort |
Karsten, Ulf |
title |
Temperature and light requirements for growth of two diatom species (Bacillariophyceae) isolated from an Arctic macroalga |
title_short |
Temperature and light requirements for growth of two diatom species (Bacillariophyceae) isolated from an Arctic macroalga |
title_full |
Temperature and light requirements for growth of two diatom species (Bacillariophyceae) isolated from an Arctic macroalga |
title_fullStr |
Temperature and light requirements for growth of two diatom species (Bacillariophyceae) isolated from an Arctic macroalga |
title_full_unstemmed |
Temperature and light requirements for growth of two diatom species (Bacillariophyceae) isolated from an Arctic macroalga |
title_sort |
temperature and light requirements for growth of two diatom species (bacillariophyceae) isolated from an arctic macroalga |
publishDate |
2005 |
url |
https://epic.awi.de/id/eprint/13851/ https://doi.org/10.1007/s00300-005-0078-1 https://hdl.handle.net/10013/epic.24214 |
geographic |
Antarctic Arctic Norway |
geographic_facet |
Antarctic Arctic Norway |
genre |
Antarc* Antarctic Arctic Arctic Kongsfjord* Kongsfjorden Polar Biology Spitsbergen |
genre_facet |
Antarc* Antarctic Arctic Arctic Kongsfjord* Kongsfjorden Polar Biology Spitsbergen |
op_source |
EPIC3Polar Biology, ISSN: 0722-4060 |
op_relation |
Karsten, U. (2005) Temperature and light requirements for growth of two diatom species (Bacillariophyceae) isolated from an Arctic macroalga , Polar Biology . doi:10.1007/s00300-005-0078-1 <https://doi.org/10.1007/s00300-005-0078-1> , hdl:10013/epic.24214 |
op_doi |
https://doi.org/10.1007/s00300-005-0078-1 |
container_title |
Polar Biology |
container_volume |
29 |
container_issue |
6 |
container_start_page |
476 |
op_container_end_page |
486 |
_version_ |
1776196937424306176 |