Applying DNA Techniques to the identification of the species of dressed toasted eel products.
To differentiate the species of processed eel products, the gene identification of four fresh eel species was first established and the species of eel products collected from markets were investigated. Polymerase Chain Reaction (PCR) and sequence analysis were used to determine the genetic variation...
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ftasiauniv:oai:localhost:310904400/7794 2023-05-15T13:26:59+02:00 Applying DNA Techniques to the identification of the species of dressed toasted eel products. Hwang, D. F.; H. C. Jen; Y. W. Hsieh;C.Y. shiau 2004-09 http://asiair.asia.edu.tw/ir/handle/310904400/7794 http://asiair.asia.edu.tw/ir/bitstream/310904400/7794/1/index.html en_US eng Asia University Journal of Agricultural and Food Chemistry, 52(19): 5972-5977. http://asiair.asia.edu.tw/ir/handle/310904400/7794 http://asiair.asia.edu.tw/ir/bitstream/310904400/7794/1/index.html 2004 ftasiauniv 2019-11-30T11:55:22Z To differentiate the species of processed eel products, the gene identification of four fresh eel species was first established and the species of eel products collected from markets were investigated. Polymerase Chain Reaction (PCR) and sequence analysis were used to determine the genetic variation in a 362-nucleotide region of the mitochondrial cytochrome b gene in four fresh eels including Anguilla japonica, Anguilla anguilla, Anguilla rostrata, and Muraenesox cinereus. It was found that each eel species had a unique genotype, which was no different among fresh, frozen, and sterilized meats. The restriction enzyme Hinfl could differentiate the species of A. japonica and A. rostrata but could not differentiate A. anguilla and M. cinereus. Another restriction enzyme, Sau96 I, was valuable in the differentiation of M. cinereus from the other three species of Anguilla. By applying PCR and restriction enzymes, the species of 12 commercial eel products were identified as A. japonica (9 samples), A. anguilla (2), and A. rostrata (1). This indicated that the sequence and restriction enzyme cutting site analyses were very usable to authenticate species of different processed eel products. Other/Unknown Material Anguilla anguilla ASIA University, Taiwan: Instiutional Repository (ASIAIR) |
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Open Polar |
collection |
ASIA University, Taiwan: Instiutional Repository (ASIAIR) |
op_collection_id |
ftasiauniv |
language |
English |
description |
To differentiate the species of processed eel products, the gene identification of four fresh eel species was first established and the species of eel products collected from markets were investigated. Polymerase Chain Reaction (PCR) and sequence analysis were used to determine the genetic variation in a 362-nucleotide region of the mitochondrial cytochrome b gene in four fresh eels including Anguilla japonica, Anguilla anguilla, Anguilla rostrata, and Muraenesox cinereus. It was found that each eel species had a unique genotype, which was no different among fresh, frozen, and sterilized meats. The restriction enzyme Hinfl could differentiate the species of A. japonica and A. rostrata but could not differentiate A. anguilla and M. cinereus. Another restriction enzyme, Sau96 I, was valuable in the differentiation of M. cinereus from the other three species of Anguilla. By applying PCR and restriction enzymes, the species of 12 commercial eel products were identified as A. japonica (9 samples), A. anguilla (2), and A. rostrata (1). This indicated that the sequence and restriction enzyme cutting site analyses were very usable to authenticate species of different processed eel products. |
author |
Hwang, D. F.; H. C. Jen; Y. W. Hsieh;C.Y. shiau |
spellingShingle |
Hwang, D. F.; H. C. Jen; Y. W. Hsieh;C.Y. shiau Applying DNA Techniques to the identification of the species of dressed toasted eel products. |
author_facet |
Hwang, D. F.; H. C. Jen; Y. W. Hsieh;C.Y. shiau |
author_sort |
Hwang, D. F.; H. C. Jen; Y. W. Hsieh;C.Y. shiau |
title |
Applying DNA Techniques to the identification of the species of dressed toasted eel products. |
title_short |
Applying DNA Techniques to the identification of the species of dressed toasted eel products. |
title_full |
Applying DNA Techniques to the identification of the species of dressed toasted eel products. |
title_fullStr |
Applying DNA Techniques to the identification of the species of dressed toasted eel products. |
title_full_unstemmed |
Applying DNA Techniques to the identification of the species of dressed toasted eel products. |
title_sort |
applying dna techniques to the identification of the species of dressed toasted eel products. |
publisher |
Asia University |
publishDate |
2004 |
url |
http://asiair.asia.edu.tw/ir/handle/310904400/7794 http://asiair.asia.edu.tw/ir/bitstream/310904400/7794/1/index.html |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
Journal of Agricultural and Food Chemistry, 52(19): 5972-5977. http://asiair.asia.edu.tw/ir/handle/310904400/7794 http://asiair.asia.edu.tw/ir/bitstream/310904400/7794/1/index.html |
_version_ |
1766395734683287552 |