A barnavirus sequence mined from a transcriptome of the Antarctic pearlwort Colobanthus quitensis

• Published in: Archives of Virology
• Main Authors: Nibert, Max L., Manny, Austin R., Debat, Humberto Julio, Firth, Andrew E., Bertini, Laura, Caruso, Carla
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2018
• Subjects: Genética; Secuencia Nucleotídica; Virus de las Plantas; Genetics; Nucleotide Sequence; Plant Viruses; Barnavirus; Colobanthus quitensis; Secuencia Genética; Antarc*; Antarctic
• Online Access: https://hdl.handle.net/20.500.12123/2284; https://link.springer.com/article/10.1007%2Fs00705-018-3794-x; https://doi.org/10.1007/s00705-018-3794-x

Because so few viruses in the family Barnaviridae have been reported, we searched for more of them in public sequence databases. Here, we report the complete coding sequence of Colobanthus quitensis associated barnavirus 1, mined from a transcriptome of the Antarctic pearlwort Colobanthus quitensis. The 4.2-kb plus-strand sequence of this virus encompasses four main open reading frames (ORFs), as expected for barnaviruses, including ORFs for a protease-containing polyprotein, an RNA-dependent RNA polymerase whose translation appears to rely on − 1 ribosomal frameshifting, and a capsid protein that is likely to be translated from a subgenomic RNA. The possible derivation of this virus from a fungus associated with C. quitensis is discussed. Instituto de Patología Vegetal Fil: Nibert, Max L. Harvard Medical School. Department of Microbiology and Immunobiology; Estados Unidos. Harvard University. Ph.D. Program in Virology; Estados Unidos Fil: Manny, Austin R. Harvard University. Ph.D. Program in Virology; Estados Unidos Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Firth, Andrew E. University of Cambridge. Division of Virology. Department of Pathology; Gran Bretaña Fil: Bertini, Laura. Universita degli Studi della Tuscia. Department of Ecological and Biological Sciences; Italia Fil: Caruso, Carla. Universita degli Studi della Tuscia. Department of Ecological and Biological Sciences; Italia