Effect of freezing rate on motility, adenosine triphosphate content and fertilizability in beluga sturgeon (Huso huso) spermatozoa
Broodstock selection programs are currently underway for sturgeon species. To complement and further these selection programs we need to develop sperm cryopreservation procedures. In the present study, we describe the effects of freezing rate (-10 C, -15 C,-20 C, -30 C and -40 C/min) on gamete quali...
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ftardabiluniv:oai:eprints.arums.ac.ir:6861 2023-07-30T04:02:38+02:00 Effect of freezing rate on motility, adenosine triphosphate content and fertilizability in beluga sturgeon (Huso huso) spermatozoa گلشاهی, کریم آراملی, محمد صادق نظری, رجب محمد آراملی, سلیم 1394-03-06 text https://eprints.arums.ac.ir/6861/ https://eprints.arums.ac.ir/6861/1/1-s2.0-S0011224015000292-main.pdf http://www.sciencedirect.com/science/journal/00112240/70/2 en eng Elsevier https://eprints.arums.ac.ir/6861/1/1-s2.0-S0011224015000292-main.pdf گلشاهی, کریم ، آراملی, محمد صادق ، نظری, رجب محمد ، آراملی, سلیم (1394) تاثیر میزان انجماد بر روی تحرک، محتوای آدنوزین تری فسفات و قابلیت باروری در اسپرم فیل ماهی. Cryobiology ــ 70 (2). ص.ص.170-174. شاپا 2240-0011 QT فیزیولوژی QU بیوشیمی مقاله PeerReviewed 1394 ftardabiluniv 2023-07-12T20:27:08Z Broodstock selection programs are currently underway for sturgeon species. To complement and further these selection programs we need to develop sperm cryopreservation procedures. In the present study, we describe the effects of freezing rate (-10 C, -15 C,-20 C, -30 C and -40 C/min) on gamete quality characteristics (i.e., duration of motility (s), motility percentage (%), ATP content (nmol/108 cells), fertilization rate (%), and hatching rate (%)) in beluga sturgeon, Huso huso. After sampling, beluga sturgeon sperm were diluted in an extender composed of 23.4 mM sucrose, 0.25 mM KCl, and 30 mM Tris–HCl, pH 8.0 containing 10% methanol and subsequently frozen in a programmable freezer. Sperm frozen at-40 C/min resulted in means for duration of motility (134 s), motility percentage (69%), ATP concentration 4.8 )nmol/108 cells), fertilization rate (72%) and hatching rate (65%) that were higher (P < 0.05) than those for slower cooling rates. Based on our results, -40 C/min was the best freezing rate (among those tested) for cryopreservation of beluga sturgeon sperm. Text Beluga Beluga* Ardabil University of Medical Sciences: Research Information Management System |
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QT فیزیولوژی QU بیوشیمی |
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QT فیزیولوژی QU بیوشیمی گلشاهی, کریم آراملی, محمد صادق نظری, رجب محمد آراملی, سلیم Effect of freezing rate on motility, adenosine triphosphate content and fertilizability in beluga sturgeon (Huso huso) spermatozoa |
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QT فیزیولوژی QU بیوشیمی |
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Broodstock selection programs are currently underway for sturgeon species. To complement and further these selection programs we need to develop sperm cryopreservation procedures. In the present study, we describe the effects of freezing rate (-10 C, -15 C,-20 C, -30 C and -40 C/min) on gamete quality characteristics (i.e., duration of motility (s), motility percentage (%), ATP content (nmol/108 cells), fertilization rate (%), and hatching rate (%)) in beluga sturgeon, Huso huso. After sampling, beluga sturgeon sperm were diluted in an extender composed of 23.4 mM sucrose, 0.25 mM KCl, and 30 mM Tris–HCl, pH 8.0 containing 10% methanol and subsequently frozen in a programmable freezer. Sperm frozen at-40 C/min resulted in means for duration of motility (134 s), motility percentage (69%), ATP concentration 4.8 )nmol/108 cells), fertilization rate (72%) and hatching rate (65%) that were higher (P < 0.05) than those for slower cooling rates. Based on our results, -40 C/min was the best freezing rate (among those tested) for cryopreservation of beluga sturgeon sperm. |
format |
Text |
author |
گلشاهی, کریم آراملی, محمد صادق نظری, رجب محمد آراملی, سلیم |
author_facet |
گلشاهی, کریم آراملی, محمد صادق نظری, رجب محمد آراملی, سلیم |
author_sort |
گلشاهی, کریم |
title |
Effect of freezing rate on motility, adenosine triphosphate content and fertilizability in beluga sturgeon (Huso huso) spermatozoa |
title_short |
Effect of freezing rate on motility, adenosine triphosphate content and fertilizability in beluga sturgeon (Huso huso) spermatozoa |
title_full |
Effect of freezing rate on motility, adenosine triphosphate content and fertilizability in beluga sturgeon (Huso huso) spermatozoa |
title_fullStr |
Effect of freezing rate on motility, adenosine triphosphate content and fertilizability in beluga sturgeon (Huso huso) spermatozoa |
title_full_unstemmed |
Effect of freezing rate on motility, adenosine triphosphate content and fertilizability in beluga sturgeon (Huso huso) spermatozoa |
title_sort |
effect of freezing rate on motility, adenosine triphosphate content and fertilizability in beluga sturgeon (huso huso) spermatozoa |
publisher |
Elsevier |
publishDate |
1394 |
url |
https://eprints.arums.ac.ir/6861/ https://eprints.arums.ac.ir/6861/1/1-s2.0-S0011224015000292-main.pdf http://www.sciencedirect.com/science/journal/00112240/70/2 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_relation |
https://eprints.arums.ac.ir/6861/1/1-s2.0-S0011224015000292-main.pdf گلشاهی, کریم ، آراملی, محمد صادق ، نظری, رجب محمد ، آراملی, سلیم (1394) تاثیر میزان انجماد بر روی تحرک، محتوای آدنوزین تری فسفات و قابلیت باروری در اسپرم فیل ماهی. Cryobiology ــ 70 (2). ص.ص.170-174. شاپا 2240-0011 |
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1772813453107396608 |