Occurrence, genetic characterization, and zoonotic importance of Giardia duodenalis in various species of rodents (Mus musculus, Rattus norvegicus, and Rattus rattus)
Giardia duodenalis is a well-known flagellated parasite and the causative agent of protozoal diarrhea in animals and humans worldwide. Current study was aimed at determination of G. duodenalis prevalence, genetic variation and zoonotic significance in various species of rodents in Shiraz, southweste...
Published in: | Comparative Immunology, Microbiology and Infectious Diseases |
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ftardabiluniv:oai:eprints.arums.ac.ir:15896 2023-07-30T04:06:30+02:00 Occurrence, genetic characterization, and zoonotic importance of Giardia duodenalis in various species of rodents (Mus musculus, Rattus norvegicus, and Rattus rattus) اصغری, علی معتضدیان, محمد حسین اصغری, قاسم شمسی, لیلا سرکاری, بهادر شهابی, سعید محمدی قلعه بین, بهنام 1401-04-29 text https://eprints.arums.ac.ir/15896/ https://eprints.arums.ac.ir/15896/1/Giardia%20Rodents.%20Mohammadi.pdf https://www.sciencedirect.com/science/article/pii/S0147957122000698 https://doi.org/10.1016/j.cimid.2022.101812 fa per Elsevier https://eprints.arums.ac.ir/15896/1/Giardia%20Rodents.%20Mohammadi.pdf اصغری, علی ، معتضدیان, محمد حسین ، اصغری, قاسم ، شمسی, لیلا ، سرکاری, بهادر ، شهابی, سعید ، محمدی قلعه بین, بهنام (1401) وجود، خصوصیات ژنتیکی و اهمیت زئونوز بودن ژیاردیا دئودنالیس در گونه های مختلف جوندگان (موس موسکولوس، راتوس نروژیکوس و راتوس راتوس). Comparative Immunology, Microbiology and Infectious Diseases ــ 85 (101812). ص.ص.1-6. شاپا 1878-1667 QX انگل شناسی مقاله PeerReviewed 1401 ftardabiluniv https://doi.org/10.1016/j.cimid.2022.101812 2023-07-12T20:31:35Z Giardia duodenalis is a well-known flagellated parasite and the causative agent of protozoal diarrhea in animals and humans worldwide. Current study was aimed at determination of G. duodenalis prevalence, genetic variation and zoonotic significance in various species of rodents in Shiraz, southwestern Iran. In brief, 120 fecal specimens were collected from rodents (Rattus rattus, Rattus norvegicus, and Mus musculus) during May up to November 2021 and microscopically examined for Giardia cysts. Further molecular characterization of positive samples was done by nested-PCR, followed by nucleotide sequencing of the triose phosphate isomerase (tpi) gene. A total prevalence of 3.3% (4/120) was observed in rodents, with highest rate in black rats [5% (2/40)]. Regarding brown rats and house mice, only one sample was found to be positive, showing 2.5% and 2.5% prevalence, respectively. It is noteworthy that Giardia B and G assemblages were found in black rats (one case/genotype), whereas the only positive samples from brown rats and house mice were characterized as assemblage G. The major findings of the present study were the presence of both zoonotic and non-zoonotic Giardia assemblages in examined rats in Shiraz and the potential of black rats to harbor Giardia infection to humans. These concerns should be taken seriously in terms of public health. Nevertheless, the true epidemiology and assemblage distribution of Giardia is still open to question. Text Rattus rattus Ardabil University of Medical Sciences: Research Information Management System Comparative Immunology, Microbiology and Infectious Diseases 85 101812 |
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QX انگل شناسی اصغری, علی معتضدیان, محمد حسین اصغری, قاسم شمسی, لیلا سرکاری, بهادر شهابی, سعید محمدی قلعه بین, بهنام Occurrence, genetic characterization, and zoonotic importance of Giardia duodenalis in various species of rodents (Mus musculus, Rattus norvegicus, and Rattus rattus) |
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QX انگل شناسی |
description |
Giardia duodenalis is a well-known flagellated parasite and the causative agent of protozoal diarrhea in animals and humans worldwide. Current study was aimed at determination of G. duodenalis prevalence, genetic variation and zoonotic significance in various species of rodents in Shiraz, southwestern Iran. In brief, 120 fecal specimens were collected from rodents (Rattus rattus, Rattus norvegicus, and Mus musculus) during May up to November 2021 and microscopically examined for Giardia cysts. Further molecular characterization of positive samples was done by nested-PCR, followed by nucleotide sequencing of the triose phosphate isomerase (tpi) gene. A total prevalence of 3.3% (4/120) was observed in rodents, with highest rate in black rats [5% (2/40)]. Regarding brown rats and house mice, only one sample was found to be positive, showing 2.5% and 2.5% prevalence, respectively. It is noteworthy that Giardia B and G assemblages were found in black rats (one case/genotype), whereas the only positive samples from brown rats and house mice were characterized as assemblage G. The major findings of the present study were the presence of both zoonotic and non-zoonotic Giardia assemblages in examined rats in Shiraz and the potential of black rats to harbor Giardia infection to humans. These concerns should be taken seriously in terms of public health. Nevertheless, the true epidemiology and assemblage distribution of Giardia is still open to question. |
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اصغری, علی معتضدیان, محمد حسین اصغری, قاسم شمسی, لیلا سرکاری, بهادر شهابی, سعید محمدی قلعه بین, بهنام |
author_facet |
اصغری, علی معتضدیان, محمد حسین اصغری, قاسم شمسی, لیلا سرکاری, بهادر شهابی, سعید محمدی قلعه بین, بهنام |
author_sort |
اصغری, علی |
title |
Occurrence, genetic characterization, and zoonotic importance of Giardia duodenalis in various species of rodents (Mus musculus, Rattus norvegicus, and Rattus rattus) |
title_short |
Occurrence, genetic characterization, and zoonotic importance of Giardia duodenalis in various species of rodents (Mus musculus, Rattus norvegicus, and Rattus rattus) |
title_full |
Occurrence, genetic characterization, and zoonotic importance of Giardia duodenalis in various species of rodents (Mus musculus, Rattus norvegicus, and Rattus rattus) |
title_fullStr |
Occurrence, genetic characterization, and zoonotic importance of Giardia duodenalis in various species of rodents (Mus musculus, Rattus norvegicus, and Rattus rattus) |
title_full_unstemmed |
Occurrence, genetic characterization, and zoonotic importance of Giardia duodenalis in various species of rodents (Mus musculus, Rattus norvegicus, and Rattus rattus) |
title_sort |
occurrence, genetic characterization, and zoonotic importance of giardia duodenalis in various species of rodents (mus musculus, rattus norvegicus, and rattus rattus) |
publisher |
Elsevier |
publishDate |
1401 |
url |
https://eprints.arums.ac.ir/15896/ https://eprints.arums.ac.ir/15896/1/Giardia%20Rodents.%20Mohammadi.pdf https://www.sciencedirect.com/science/article/pii/S0147957122000698 https://doi.org/10.1016/j.cimid.2022.101812 |
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Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
https://eprints.arums.ac.ir/15896/1/Giardia%20Rodents.%20Mohammadi.pdf اصغری, علی ، معتضدیان, محمد حسین ، اصغری, قاسم ، شمسی, لیلا ، سرکاری, بهادر ، شهابی, سعید ، محمدی قلعه بین, بهنام (1401) وجود، خصوصیات ژنتیکی و اهمیت زئونوز بودن ژیاردیا دئودنالیس در گونه های مختلف جوندگان (موس موسکولوس، راتوس نروژیکوس و راتوس راتوس). Comparative Immunology, Microbiology and Infectious Diseases ــ 85 (101812). ص.ص.1-6. شاپا 1878-1667 |
op_doi |
https://doi.org/10.1016/j.cimid.2022.101812 |
container_title |
Comparative Immunology, Microbiology and Infectious Diseases |
container_volume |
85 |
container_start_page |
101812 |
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