Construction and screening of a functional metagenomic library to identify novel enzymes produced by Antarctic bacteria
A metagenomic fosmid library of approximately 52 000 clones was constructed to identify functional genes encoding cold-adapted enzymes. Metagenomic DNA was extracted from a sample of glacial meltwater, collected on the Antarctic Peninsula during the ANTARKOS XXIX Expedition during the austral summer...
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Polar Research Institute of China - PRIC
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ftarcticportal:oai:generic.eprints.org:2563 2023-11-12T03:59:41+01:00 Construction and screening of a functional metagenomic library to identify novel enzymes produced by Antarctic bacteria Ferrés, Ignacio Amarelle, Vanesa Noya, Francisco Fabiano, Elena 2015-03 application/pdf http://library.arcticportal.org/2563/ http://library.arcticportal.org/2563/1/A20150112.pdf en eng Polar Research Institute of China - PRIC http://library.arcticportal.org/2563/1/A20150112.pdf Ferrés, Ignacio and Amarelle, Vanesa and Noya, Francisco and Fabiano, Elena (2015) Construction and screening of a functional metagenomic library to identify novel enzymes produced by Antarctic bacteria. Advances in Polar Science, 26 (1). pp. 96-101. Article PeerReviewed 2015 ftarcticportal 2023-11-01T23:54:37Z A metagenomic fosmid library of approximately 52 000 clones was constructed to identify functional genes encoding cold-adapted enzymes. Metagenomic DNA was extracted from a sample of glacial meltwater, collected on the Antarctic Peninsula during the ANTARKOS XXIX Expedition during the austral summer of 2012–2013. Each clone contained an insert of about 35–40 kb, so the library represented almost 2 Gb of genetic information from metagenomic DNA. Activity-driven screening was used to detect the cold-adapted functions expressed by the library. Fifty lipase/esterase and two cellulase-producing clones were isolated, and two clones able to grow on Avicel® as the sole carbon source. Interestingly, three clones formed a brown precipitate in the presence of manganese (II). Accumulation of manganese oxides was determined with a leucoberbelin blue assay, indicating that these three clones had manganese-oxidizing activity. To the best of our knowledge, this is the first report of a manganese oxidase activity detected with a functional metagenomic strategy. Article in Journal/Newspaper Advances in Polar Science Antarc* Antarctic Antarctic Peninsula Polar Science Polar Science Arctic Portal Library |
institution |
Open Polar |
collection |
Arctic Portal Library |
op_collection_id |
ftarcticportal |
language |
English |
description |
A metagenomic fosmid library of approximately 52 000 clones was constructed to identify functional genes encoding cold-adapted enzymes. Metagenomic DNA was extracted from a sample of glacial meltwater, collected on the Antarctic Peninsula during the ANTARKOS XXIX Expedition during the austral summer of 2012–2013. Each clone contained an insert of about 35–40 kb, so the library represented almost 2 Gb of genetic information from metagenomic DNA. Activity-driven screening was used to detect the cold-adapted functions expressed by the library. Fifty lipase/esterase and two cellulase-producing clones were isolated, and two clones able to grow on Avicel® as the sole carbon source. Interestingly, three clones formed a brown precipitate in the presence of manganese (II). Accumulation of manganese oxides was determined with a leucoberbelin blue assay, indicating that these three clones had manganese-oxidizing activity. To the best of our knowledge, this is the first report of a manganese oxidase activity detected with a functional metagenomic strategy. |
format |
Article in Journal/Newspaper |
author |
Ferrés, Ignacio Amarelle, Vanesa Noya, Francisco Fabiano, Elena |
spellingShingle |
Ferrés, Ignacio Amarelle, Vanesa Noya, Francisco Fabiano, Elena Construction and screening of a functional metagenomic library to identify novel enzymes produced by Antarctic bacteria |
author_facet |
Ferrés, Ignacio Amarelle, Vanesa Noya, Francisco Fabiano, Elena |
author_sort |
Ferrés, Ignacio |
title |
Construction and screening of a functional metagenomic library to identify novel enzymes produced by Antarctic bacteria |
title_short |
Construction and screening of a functional metagenomic library to identify novel enzymes produced by Antarctic bacteria |
title_full |
Construction and screening of a functional metagenomic library to identify novel enzymes produced by Antarctic bacteria |
title_fullStr |
Construction and screening of a functional metagenomic library to identify novel enzymes produced by Antarctic bacteria |
title_full_unstemmed |
Construction and screening of a functional metagenomic library to identify novel enzymes produced by Antarctic bacteria |
title_sort |
construction and screening of a functional metagenomic library to identify novel enzymes produced by antarctic bacteria |
publisher |
Polar Research Institute of China - PRIC |
publishDate |
2015 |
url |
http://library.arcticportal.org/2563/ http://library.arcticportal.org/2563/1/A20150112.pdf |
genre |
Advances in Polar Science Antarc* Antarctic Antarctic Peninsula Polar Science Polar Science |
genre_facet |
Advances in Polar Science Antarc* Antarctic Antarctic Peninsula Polar Science Polar Science |
op_relation |
http://library.arcticportal.org/2563/1/A20150112.pdf Ferrés, Ignacio and Amarelle, Vanesa and Noya, Francisco and Fabiano, Elena (2015) Construction and screening of a functional metagenomic library to identify novel enzymes produced by Antarctic bacteria. Advances in Polar Science, 26 (1). pp. 96-101. |
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1782332935445151744 |