Identification of Antarctic culturable bacteria able to produce diverse enzymes of potential biotechnological interest
It is estimated that more than three quarters of the Earth’s biosphere is in perennially cold environments. Despite the extreme environmental conditions of desiccation and freezing, microbes can colonize these habitats through the adaptation of metabolic functions and the synthesis of structurally a...
Main Authors: | , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Polar Research Institute of China - PRIC
2015
|
Subjects: | |
Online Access: | http://library.arcticportal.org/2560/ http://library.arcticportal.org/2560/1/A20150109.pdf |
id |
ftarcticportal:oai:generic.eprints.org:2560 |
---|---|
record_format |
openpolar |
spelling |
ftarcticportal:oai:generic.eprints.org:2560 2023-11-12T03:59:41+01:00 Identification of Antarctic culturable bacteria able to produce diverse enzymes of potential biotechnological interest Ferrés, Ignacio Amarelle, Vanesa Noya, Francisco Fabiano, Elena 2015-03 application/pdf http://library.arcticportal.org/2560/ http://library.arcticportal.org/2560/1/A20150109.pdf en eng Polar Research Institute of China - PRIC http://library.arcticportal.org/2560/1/A20150109.pdf Ferrés, Ignacio and Amarelle, Vanesa and Noya, Francisco and Fabiano, Elena (2015) Identification of Antarctic culturable bacteria able to produce diverse enzymes of potential biotechnological interest. Advances in Polar Science, 26 (1). pp. 71-79. Cryosphere Fauna Article PeerReviewed 2015 ftarcticportal 2023-11-01T23:54:37Z It is estimated that more than three quarters of the Earth’s biosphere is in perennially cold environments. Despite the extreme environmental conditions of desiccation and freezing, microbes can colonize these habitats through the adaptation of metabolic functions and the synthesis of structurally adapted enzymes. Enzymes within psychrophilic microbes exhibit high specific activity at low and moderate temperature, with low thermostability. In this study we used a classic microbiological approach to isolate Antarctic bacteria with cellulolytic, lipolytic, and ligninolytic activities. From 15 different environmental samples, we generated a collection of approximately 800 bacterial isolates that could grow on R2A or Marine medium at 4°C. This collection was then screened for the presence of the three types of activity at 4°C. We found that 47.7% of the isolates displayed lipolytic activity, 10.2% had cellulase/xylanase activity, and 7.7% showed guaiacol oxidase activity. Of these, 10% displayed two different types of activity, while 0.25% displayed all three types of activity. Our results indicate that cold environments represent outstanding resources for bioprospecting and the study of enzymatic adaptation. Article in Journal/Newspaper Advances in Polar Science Antarc* Antarctic Polar Science Polar Science Arctic Portal Library |
institution |
Open Polar |
collection |
Arctic Portal Library |
op_collection_id |
ftarcticportal |
language |
English |
topic |
Cryosphere Fauna |
spellingShingle |
Cryosphere Fauna Ferrés, Ignacio Amarelle, Vanesa Noya, Francisco Fabiano, Elena Identification of Antarctic culturable bacteria able to produce diverse enzymes of potential biotechnological interest |
topic_facet |
Cryosphere Fauna |
description |
It is estimated that more than three quarters of the Earth’s biosphere is in perennially cold environments. Despite the extreme environmental conditions of desiccation and freezing, microbes can colonize these habitats through the adaptation of metabolic functions and the synthesis of structurally adapted enzymes. Enzymes within psychrophilic microbes exhibit high specific activity at low and moderate temperature, with low thermostability. In this study we used a classic microbiological approach to isolate Antarctic bacteria with cellulolytic, lipolytic, and ligninolytic activities. From 15 different environmental samples, we generated a collection of approximately 800 bacterial isolates that could grow on R2A or Marine medium at 4°C. This collection was then screened for the presence of the three types of activity at 4°C. We found that 47.7% of the isolates displayed lipolytic activity, 10.2% had cellulase/xylanase activity, and 7.7% showed guaiacol oxidase activity. Of these, 10% displayed two different types of activity, while 0.25% displayed all three types of activity. Our results indicate that cold environments represent outstanding resources for bioprospecting and the study of enzymatic adaptation. |
format |
Article in Journal/Newspaper |
author |
Ferrés, Ignacio Amarelle, Vanesa Noya, Francisco Fabiano, Elena |
author_facet |
Ferrés, Ignacio Amarelle, Vanesa Noya, Francisco Fabiano, Elena |
author_sort |
Ferrés, Ignacio |
title |
Identification of Antarctic culturable bacteria able to produce diverse enzymes of potential biotechnological interest |
title_short |
Identification of Antarctic culturable bacteria able to produce diverse enzymes of potential biotechnological interest |
title_full |
Identification of Antarctic culturable bacteria able to produce diverse enzymes of potential biotechnological interest |
title_fullStr |
Identification of Antarctic culturable bacteria able to produce diverse enzymes of potential biotechnological interest |
title_full_unstemmed |
Identification of Antarctic culturable bacteria able to produce diverse enzymes of potential biotechnological interest |
title_sort |
identification of antarctic culturable bacteria able to produce diverse enzymes of potential biotechnological interest |
publisher |
Polar Research Institute of China - PRIC |
publishDate |
2015 |
url |
http://library.arcticportal.org/2560/ http://library.arcticportal.org/2560/1/A20150109.pdf |
genre |
Advances in Polar Science Antarc* Antarctic Polar Science Polar Science |
genre_facet |
Advances in Polar Science Antarc* Antarctic Polar Science Polar Science |
op_relation |
http://library.arcticportal.org/2560/1/A20150109.pdf Ferrés, Ignacio and Amarelle, Vanesa and Noya, Francisco and Fabiano, Elena (2015) Identification of Antarctic culturable bacteria able to produce diverse enzymes of potential biotechnological interest. Advances in Polar Science, 26 (1). pp. 71-79. |
_version_ |
1782332912998285312 |