Detection of integron integrase genes on King George Island, Antarctica
The presence and diversity of class 1 integrase gene (intI) sequences were evaluated by PCR using previously designed primers. Two clone libraries were constructed from DNA in sediment and microbial mat samples collected on Fildes Peninsula, King George Island, Antarctica. The libraries constructed...
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ftarcticportal:oai:generic.eprints.org:2554 2023-11-05T03:31:01+01:00 Detection of integron integrase genes on King George Island, Antarctica Antelo, Verónica Romero, Héctor Batista, Silvia 2015-03 application/pdf http://library.arcticportal.org/2554/ http://library.arcticportal.org/2554/1/A20150104.pdf en eng Polar Research Institute of China - PRIC http://library.arcticportal.org/2554/1/A20150104.pdf Antelo, Verónica and Romero, Héctor and Batista, Silvia (2015) Detection of integron integrase genes on King George Island, Antarctica. Advances in Polar Science, 26 (1). pp. 30-37. Fauna Article PeerReviewed 2015 ftarcticportal 2023-10-11T22:54:25Z The presence and diversity of class 1 integrase gene (intI) sequences were evaluated by PCR using previously designed primers. Two clone libraries were constructed from DNA in sediment and microbial mat samples collected on Fildes Peninsula, King George Island, Antarctica. The libraries constructed from samples collected at Halfthree Point (HP) and Norma Cove (NC) contained 62 and 36 partial intI sequences, respectively. These sequences clustered into 10 different groups with < 95% amino acid identity. Alignment of the deduced amino acid sequences with those from recognized integron-encoded integrases demonstrated the presence of highly conserved motifs characteristic of intI integrases. The HP library contained 42 nucleotide sequences identical to the class 1 intI gene found in a collection of trimethoprim-resistant (Tmpr) Antarctic Enterobacter sp. isolates, previously collected in the same area. These integrons, located on plasmids, had a genetic organization similar to that of pKOX105 from Klebsiella oxytoca. The 20 remaining HP and NC library sequences were similar to integrase sequences previously determined in a metagenomic analysis of environmental samples. We have demonstrated the presence of integron integrase genes in Antarctic sediment samples. About half these genes were very similar to the class 1 integrons found in humanassociated microbiota, suggesting that they originated from human-dominated ecosystems. The remaining integrase genes were probably associated with endemic bacteria. Article in Journal/Newspaper Advances in Polar Science Antarc* Antarctic Antarctica King George Island Polar Science Polar Science Arctic Portal Library |
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Fauna Antelo, Verónica Romero, Héctor Batista, Silvia Detection of integron integrase genes on King George Island, Antarctica |
topic_facet |
Fauna |
description |
The presence and diversity of class 1 integrase gene (intI) sequences were evaluated by PCR using previously designed primers. Two clone libraries were constructed from DNA in sediment and microbial mat samples collected on Fildes Peninsula, King George Island, Antarctica. The libraries constructed from samples collected at Halfthree Point (HP) and Norma Cove (NC) contained 62 and 36 partial intI sequences, respectively. These sequences clustered into 10 different groups with < 95% amino acid identity. Alignment of the deduced amino acid sequences with those from recognized integron-encoded integrases demonstrated the presence of highly conserved motifs characteristic of intI integrases. The HP library contained 42 nucleotide sequences identical to the class 1 intI gene found in a collection of trimethoprim-resistant (Tmpr) Antarctic Enterobacter sp. isolates, previously collected in the same area. These integrons, located on plasmids, had a genetic organization similar to that of pKOX105 from Klebsiella oxytoca. The 20 remaining HP and NC library sequences were similar to integrase sequences previously determined in a metagenomic analysis of environmental samples. We have demonstrated the presence of integron integrase genes in Antarctic sediment samples. About half these genes were very similar to the class 1 integrons found in humanassociated microbiota, suggesting that they originated from human-dominated ecosystems. The remaining integrase genes were probably associated with endemic bacteria. |
format |
Article in Journal/Newspaper |
author |
Antelo, Verónica Romero, Héctor Batista, Silvia |
author_facet |
Antelo, Verónica Romero, Héctor Batista, Silvia |
author_sort |
Antelo, Verónica |
title |
Detection of integron integrase genes on King George Island, Antarctica |
title_short |
Detection of integron integrase genes on King George Island, Antarctica |
title_full |
Detection of integron integrase genes on King George Island, Antarctica |
title_fullStr |
Detection of integron integrase genes on King George Island, Antarctica |
title_full_unstemmed |
Detection of integron integrase genes on King George Island, Antarctica |
title_sort |
detection of integron integrase genes on king george island, antarctica |
publisher |
Polar Research Institute of China - PRIC |
publishDate |
2015 |
url |
http://library.arcticportal.org/2554/ http://library.arcticportal.org/2554/1/A20150104.pdf |
genre |
Advances in Polar Science Antarc* Antarctic Antarctica King George Island Polar Science Polar Science |
genre_facet |
Advances in Polar Science Antarc* Antarctic Antarctica King George Island Polar Science Polar Science |
op_relation |
http://library.arcticportal.org/2554/1/A20150104.pdf Antelo, Verónica and Romero, Héctor and Batista, Silvia (2015) Detection of integron integrase genes on King George Island, Antarctica. Advances in Polar Science, 26 (1). pp. 30-37. |
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1781696334938505216 |