Studies on culture condition and extracellular hydrolase of psychrophilic bacteria from Arctic sea ice

• Main Authors: Xiaohui, Li, Yong, Yu, Huirong, Li, Lin, Zhang, Xinyin, Jiang, Daming, Ren
• Format: Article in Journal/Newspaper
• Language: English
• Published: Polar Research Institute of China - PRIC 2008
• Subjects: Oceans; Arctic; Advances in Polar Science; Polar Science; Sea ice
• Online Access: http://library.arcticportal.org/2350/; http://library.arcticportal.org/2350/1/A200801009.pdf

Arctic sea ice in the polar region provides a cold habitat for microbial community. Arctic sea ice microorganisms are revealed to be of considerable importance in basic research and potential in biotechnological application. This paper investigated the culture condition and extracellular hydrolase of 14 strains of different Arctic sea ice bacteria. The results showed that optimal growth temperature of strains is 15°C or 20°C. The optimal pH is about 8.0. They hardly grow at acid condition. 3% NaCl is necessary for better growth. These strains have different abilities in producing amylase, protease, cellulase and lipase. Pseudoalteronomas sp. Bsi429 and Pseudoalteronomas sp. Bsi539 produced both cellulose, protease and lipase. These results provide a basis for further developing and exploiting the cold adapted marine enzyme resources.