Screening on DHA-producing Antarctic bacteria N-6 and its cultural conditions

• Main Authors: Botao, Zhang, Jinlai, Miao, Hanxing, Hui, Qing, Wang
• Format: Article in Journal/Newspaper
• Language: English
• Published: Polar Research Institute of China - PRIC 2006
• Subjects: Antarctic; Advances in Polar Science; Antarc*; Antarctica; Polar Science; Sea ice
• Online Access: http://library.arcticportal.org/2305/; http://library.arcticportal.org/2305/1/A200601006.pdf

In Antarctic, the geography and climate differs from those in other places, and the bacteria there have adapted well to the environment there. Two hundred strains of bacteria were isolated from the sea ice in Antarctica. The bacteria were screened for DHA by means of GC, with fish oil as the standard. Seven strains containing DHA or EPA were obtained, among which the strain of No. N-6 was outstanding and the component of DHA was identified by GC-MS. The relative content of DHA in N-6 was 8.72%, and total lipids in dry bacteria was 22.54%. The effects of some factors, including temperature, salinity and pH value, on the growth and DHA content of strain N-6 were studied. The results show that the DHA content is relatively high when in low temperature and high pH, and the bacterium is psychrophilic, alkalophilic.