Isolation and characterization of a marine bacterium producing protease from Chukchi Sea, Arctic
A gram negative bacterium Ar/W/b/75°25'N/1 producing extracellular alkaline protease was isolated from surface water of latitude 75°25'N, and longitude 162°25'W in Chukchi Sea, Arctic. The strain can grow at the temperature range from 7°C to 30°C, and grow better at 30°C. It can not g...
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Polar Research Institute of China - PRIC
2001
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ftarcticportal:oai:generic.eprints.org:2218 2023-06-11T04:03:00+02:00 Isolation and characterization of a marine bacterium producing protease from Chukchi Sea, Arctic Yinxin, Zheng Minghong, Cai Bo, Chen Jianfeng, He 2001-06 application/pdf http://library.arcticportal.org/2218/ http://library.arcticportal.org/2218/1/A200101008.pdf en eng Polar Research Institute of China - PRIC http://library.arcticportal.org/2218/1/A200101008.pdf Yinxin, Zheng and Minghong, Cai and Bo, Chen and Jianfeng, He (2001) Isolation and characterization of a marine bacterium producing protease from Chukchi Sea, Arctic. Advances in Polar Science, 12 (1). pp. 69-74. Article PeerReviewed 2001 ftarcticportal 2023-05-03T22:52:41Z A gram negative bacterium Ar/W/b/75°25'N/1 producing extracellular alkaline protease was isolated from surface water of latitude 75°25'N, and longitude 162°25'W in Chukchi Sea, Arctic. The strain can grow at the temperature range from 7°C to 30°C, and grow better at 30°C. It can not grow at 40°C. Keeping certain salinity concentration in medium is necessary for cell growth. It grows well in medium containing salinity concentration from 0.5% to 10% sodium chloride. Glucose, sucrose and soluble starch can be utilized by the strain, among which glucose is the optimal carbon source. Peptone is the optimal organic nitrogen source for cell growth and protease producing, and ammonium nitrate is the optimal inorganic nitrogen source. About 75.7% of total protease of the strain are extracellular enzyme. Optimal temperature for proteolytic activity is at 40°C. Protease of the strain keeps stable below 40°C, and shows high proteolytic activity within the pH range from 7 - 11. Article in Journal/Newspaper Advances in Polar Science Arctic Arctic Chukchi Chukchi Sea Polar Science Polar Science Arctic Portal Library Arctic Chukchi Sea |
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Open Polar |
collection |
Arctic Portal Library |
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ftarcticportal |
language |
English |
description |
A gram negative bacterium Ar/W/b/75°25'N/1 producing extracellular alkaline protease was isolated from surface water of latitude 75°25'N, and longitude 162°25'W in Chukchi Sea, Arctic. The strain can grow at the temperature range from 7°C to 30°C, and grow better at 30°C. It can not grow at 40°C. Keeping certain salinity concentration in medium is necessary for cell growth. It grows well in medium containing salinity concentration from 0.5% to 10% sodium chloride. Glucose, sucrose and soluble starch can be utilized by the strain, among which glucose is the optimal carbon source. Peptone is the optimal organic nitrogen source for cell growth and protease producing, and ammonium nitrate is the optimal inorganic nitrogen source. About 75.7% of total protease of the strain are extracellular enzyme. Optimal temperature for proteolytic activity is at 40°C. Protease of the strain keeps stable below 40°C, and shows high proteolytic activity within the pH range from 7 - 11. |
format |
Article in Journal/Newspaper |
author |
Yinxin, Zheng Minghong, Cai Bo, Chen Jianfeng, He |
spellingShingle |
Yinxin, Zheng Minghong, Cai Bo, Chen Jianfeng, He Isolation and characterization of a marine bacterium producing protease from Chukchi Sea, Arctic |
author_facet |
Yinxin, Zheng Minghong, Cai Bo, Chen Jianfeng, He |
author_sort |
Yinxin, Zheng |
title |
Isolation and characterization of a marine bacterium producing protease from Chukchi Sea, Arctic |
title_short |
Isolation and characterization of a marine bacterium producing protease from Chukchi Sea, Arctic |
title_full |
Isolation and characterization of a marine bacterium producing protease from Chukchi Sea, Arctic |
title_fullStr |
Isolation and characterization of a marine bacterium producing protease from Chukchi Sea, Arctic |
title_full_unstemmed |
Isolation and characterization of a marine bacterium producing protease from Chukchi Sea, Arctic |
title_sort |
isolation and characterization of a marine bacterium producing protease from chukchi sea, arctic |
publisher |
Polar Research Institute of China - PRIC |
publishDate |
2001 |
url |
http://library.arcticportal.org/2218/ http://library.arcticportal.org/2218/1/A200101008.pdf |
geographic |
Arctic Chukchi Sea |
geographic_facet |
Arctic Chukchi Sea |
genre |
Advances in Polar Science Arctic Arctic Chukchi Chukchi Sea Polar Science Polar Science |
genre_facet |
Advances in Polar Science Arctic Arctic Chukchi Chukchi Sea Polar Science Polar Science |
op_relation |
http://library.arcticportal.org/2218/1/A200101008.pdf Yinxin, Zheng and Minghong, Cai and Bo, Chen and Jianfeng, He (2001) Isolation and characterization of a marine bacterium producing protease from Chukchi Sea, Arctic. Advances in Polar Science, 12 (1). pp. 69-74. |
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1768388463745302528 |