Genetic and phenotypic intra-species diversity of alga Tisochrysis lutea reveals original genetic structure and domestication potential
Oceanic phytoplankton species are generally composed of many strains, with intra-species diversity consisting of genetic and phenotypic variability. Despite its importance in ecological and biotechnological contexts, this intra-species diversity and variation among strains has been little studied. W...
Published in: | European Journal of Phycology |
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Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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Informa UK Limited
2023
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Online Access: | https://archimer.ifremer.fr/doc/00855/96704/105249.pdf https://archimer.ifremer.fr/doc/00855/96704/105250.pdf https://archimer.ifremer.fr/doc/00855/96704/105251.xlsx https://archimer.ifremer.fr/doc/00855/96704/105252.pdf https://archimer.ifremer.fr/doc/00855/96704/105253.docx https://doi.org/10.1080/09670262.2023.2249073 https://archimer.ifremer.fr/doc/00855/96704/ |
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ftarchimer:oai:archimer.ifremer.fr:96704 2023-11-05T03:40:03+01:00 Genetic and phenotypic intra-species diversity of alga Tisochrysis lutea reveals original genetic structure and domestication potential Carrier, Gregory Berthelier, Jérémy Maupetit, Agathe Nicolau, Elodie Marbouty, Martial Schreiber, Nathalie Charrier, Aurelie Carcopino, Clemence Leroi, Laura Saint-jean, Bruno 2023-09-29 application/pdf https://archimer.ifremer.fr/doc/00855/96704/105249.pdf https://archimer.ifremer.fr/doc/00855/96704/105250.pdf https://archimer.ifremer.fr/doc/00855/96704/105251.xlsx https://archimer.ifremer.fr/doc/00855/96704/105252.pdf https://archimer.ifremer.fr/doc/00855/96704/105253.docx https://doi.org/10.1080/09670262.2023.2249073 https://archimer.ifremer.fr/doc/00855/96704/ eng eng Informa UK Limited https://archimer.ifremer.fr/doc/00855/96704/105249.pdf https://archimer.ifremer.fr/doc/00855/96704/105250.pdf https://archimer.ifremer.fr/doc/00855/96704/105251.xlsx https://archimer.ifremer.fr/doc/00855/96704/105252.pdf https://archimer.ifremer.fr/doc/00855/96704/105253.docx doi:10.1080/09670262.2023.2249073 https://archimer.ifremer.fr/doc/00855/96704/ info:eu-repo/semantics/openAccess restricted use European Journal of Phycology (0967-0262) (Informa UK Limited) In Press Algae antioxidant molecules genetic structure Haptophyta intra-species diversity Tisochrysis lutea text Article info:eu-repo/semantics/article 2023 ftarchimer https://doi.org/10.1080/09670262.2023.2249073 2023-10-10T22:51:09Z Oceanic phytoplankton species are generally composed of many strains, with intra-species diversity consisting of genetic and phenotypic variability. Despite its importance in ecological and biotechnological contexts, this intra-species diversity and variation among strains has been little studied. We investigated the intra-species diversity of the microalga Tisochrysis lutea, a haptophyte of the Isochrysidales order. Inter-strain diversity of T. lutea was studied because of the economic importance of the species as a feed in aquaculture and for antioxidant metabolite production, particularly fucoxanthin and other carotenoids, which have health benefits. We analysed Tara Ocean datasets which revealed that T. lutea was present in the Pacific, Atlantic and Indian Oceans but not in the Arctic or Austral Oceans. We next made phenotypic and genotypic comparisons of 11 strains of T. lutea from worldwide algal collections. All strains were cultivated in the same controlled conditions for one week, and several phenotypic traits were measured, notably antioxidant content. In parallel, the genomes of each strain were sequenced, and genetic variants identified. At the genetic and phenotypic levels, the strains were distinct from each other and our analysis revealed natural trait variations of interest in relation to further exploitation in domestication programmes. A large number of genetic variations were identified among the strains, but no major differences in genome size were observed. Moreover, limited genetic structure was observed among these strains, which could be a consequence of the complex life history of species within the Isochrysidales. Our study provides new knowledge on the intra-species diversity that should be considered in future environmental studies and breeding programmes. Highlights Tisochrysis lutea is found in many parts of the world’s oceans. T. lutea has high inter-strain phenotypic and genomic variation. Genetic structure of strains from culture collections is limited. Article in Journal/Newspaper Arctic Phytoplankton Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) European Journal of Phycology 1 18 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Algae antioxidant molecules genetic structure Haptophyta intra-species diversity Tisochrysis lutea |
spellingShingle |
Algae antioxidant molecules genetic structure Haptophyta intra-species diversity Tisochrysis lutea Carrier, Gregory Berthelier, Jérémy Maupetit, Agathe Nicolau, Elodie Marbouty, Martial Schreiber, Nathalie Charrier, Aurelie Carcopino, Clemence Leroi, Laura Saint-jean, Bruno Genetic and phenotypic intra-species diversity of alga Tisochrysis lutea reveals original genetic structure and domestication potential |
topic_facet |
Algae antioxidant molecules genetic structure Haptophyta intra-species diversity Tisochrysis lutea |
description |
Oceanic phytoplankton species are generally composed of many strains, with intra-species diversity consisting of genetic and phenotypic variability. Despite its importance in ecological and biotechnological contexts, this intra-species diversity and variation among strains has been little studied. We investigated the intra-species diversity of the microalga Tisochrysis lutea, a haptophyte of the Isochrysidales order. Inter-strain diversity of T. lutea was studied because of the economic importance of the species as a feed in aquaculture and for antioxidant metabolite production, particularly fucoxanthin and other carotenoids, which have health benefits. We analysed Tara Ocean datasets which revealed that T. lutea was present in the Pacific, Atlantic and Indian Oceans but not in the Arctic or Austral Oceans. We next made phenotypic and genotypic comparisons of 11 strains of T. lutea from worldwide algal collections. All strains were cultivated in the same controlled conditions for one week, and several phenotypic traits were measured, notably antioxidant content. In parallel, the genomes of each strain were sequenced, and genetic variants identified. At the genetic and phenotypic levels, the strains were distinct from each other and our analysis revealed natural trait variations of interest in relation to further exploitation in domestication programmes. A large number of genetic variations were identified among the strains, but no major differences in genome size were observed. Moreover, limited genetic structure was observed among these strains, which could be a consequence of the complex life history of species within the Isochrysidales. Our study provides new knowledge on the intra-species diversity that should be considered in future environmental studies and breeding programmes. Highlights Tisochrysis lutea is found in many parts of the world’s oceans. T. lutea has high inter-strain phenotypic and genomic variation. Genetic structure of strains from culture collections is limited. |
format |
Article in Journal/Newspaper |
author |
Carrier, Gregory Berthelier, Jérémy Maupetit, Agathe Nicolau, Elodie Marbouty, Martial Schreiber, Nathalie Charrier, Aurelie Carcopino, Clemence Leroi, Laura Saint-jean, Bruno |
author_facet |
Carrier, Gregory Berthelier, Jérémy Maupetit, Agathe Nicolau, Elodie Marbouty, Martial Schreiber, Nathalie Charrier, Aurelie Carcopino, Clemence Leroi, Laura Saint-jean, Bruno |
author_sort |
Carrier, Gregory |
title |
Genetic and phenotypic intra-species diversity of alga Tisochrysis lutea reveals original genetic structure and domestication potential |
title_short |
Genetic and phenotypic intra-species diversity of alga Tisochrysis lutea reveals original genetic structure and domestication potential |
title_full |
Genetic and phenotypic intra-species diversity of alga Tisochrysis lutea reveals original genetic structure and domestication potential |
title_fullStr |
Genetic and phenotypic intra-species diversity of alga Tisochrysis lutea reveals original genetic structure and domestication potential |
title_full_unstemmed |
Genetic and phenotypic intra-species diversity of alga Tisochrysis lutea reveals original genetic structure and domestication potential |
title_sort |
genetic and phenotypic intra-species diversity of alga tisochrysis lutea reveals original genetic structure and domestication potential |
publisher |
Informa UK Limited |
publishDate |
2023 |
url |
https://archimer.ifremer.fr/doc/00855/96704/105249.pdf https://archimer.ifremer.fr/doc/00855/96704/105250.pdf https://archimer.ifremer.fr/doc/00855/96704/105251.xlsx https://archimer.ifremer.fr/doc/00855/96704/105252.pdf https://archimer.ifremer.fr/doc/00855/96704/105253.docx https://doi.org/10.1080/09670262.2023.2249073 https://archimer.ifremer.fr/doc/00855/96704/ |
genre |
Arctic Phytoplankton |
genre_facet |
Arctic Phytoplankton |
op_source |
European Journal of Phycology (0967-0262) (Informa UK Limited) In Press |
op_relation |
https://archimer.ifremer.fr/doc/00855/96704/105249.pdf https://archimer.ifremer.fr/doc/00855/96704/105250.pdf https://archimer.ifremer.fr/doc/00855/96704/105251.xlsx https://archimer.ifremer.fr/doc/00855/96704/105252.pdf https://archimer.ifremer.fr/doc/00855/96704/105253.docx doi:10.1080/09670262.2023.2249073 https://archimer.ifremer.fr/doc/00855/96704/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1080/09670262.2023.2249073 |
container_title |
European Journal of Phycology |
container_start_page |
1 |
op_container_end_page |
18 |
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1781695994768916480 |