Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction
Noroviruses, the major cause of acute viral gastroenteritis, are known to bind to histo-blood group antigens (HBGAs), including ABH groups and Lewis-type epitopes, which decorate the surface of erythrocytes and epithelial cells of their host tissues. The biosynthesis of these antigens is controlled...
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ftarchimer:oai:archimer.ifremer.fr:95215 2023-08-20T04:06:03+02:00 Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction Auger, Audrey Yu, Shin-yi Guu, Shih-yun Quéméner, Agnès Euller, Gabriel Ando, Hiromune Desdouits, Marion Le Guyader, Soizick Khoo, Kay-hooi Le Pendu, Jacques Chirat, Frédéric Guerardel, Yann 2023-06 application/pdf https://archimer.ifremer.fr/doc/00840/95215/102930.pdf https://archimer.ifremer.fr/doc/00840/95215/102931.zip https://archimer.ifremer.fr/doc/00840/95215/ eng eng MDPI https://archimer.ifremer.fr/doc/00840/95215/102930.pdf https://archimer.ifremer.fr/doc/00840/95215/102931.zip https://archimer.ifremer.fr/doc/00840/95215/ info:eu-repo/semantics/openAccess restricted use Marine Drugs (1660-3397) (MDPI), 2023-06 , Vol. 21 , N. 6 , P. 342 (25p.) glycomics norovirus ligands oysters methylation text Article info:eu-repo/semantics/article 2023 ftarchimer 2023-08-01T22:51:09Z Noroviruses, the major cause of acute viral gastroenteritis, are known to bind to histo-blood group antigens (HBGAs), including ABH groups and Lewis-type epitopes, which decorate the surface of erythrocytes and epithelial cells of their host tissues. The biosynthesis of these antigens is controlled by several glycosyltransferases, the distribution and expression of which varies between tissues and individuals. The use of HBGAs as ligands by viruses is not limited to humans, as many animal species, including oysters, which synthesize similar glycan epitopes that act as a gateway for viruses, become vectors for viral infection in humans. Here, we show that different oyster species synthesize a wide range of N-glycans that share histo-blood A-antigens but differ in the expression of other terminal antigens and in their modification by O-methyl groups. In particular, we show that the N-glycans isolated from Crassostrea gigas and Ostrea edulis exhibit exquisite methylation patterns in their terminal N-acetylgalactosamine and fucose residues in terms of position and number, adding another layer of complexity to the post-translational glycosylation modifications of glycoproteins. Furthermore, modeling of the interactions between norovirus capsid proteins and carbohydrate ligands strongly suggests that methylation has the potential to fine-tune the recognition events of oysters by virus particles. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
glycomics norovirus ligands oysters methylation |
spellingShingle |
glycomics norovirus ligands oysters methylation Auger, Audrey Yu, Shin-yi Guu, Shih-yun Quéméner, Agnès Euller, Gabriel Ando, Hiromune Desdouits, Marion Le Guyader, Soizick Khoo, Kay-hooi Le Pendu, Jacques Chirat, Frédéric Guerardel, Yann Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
topic_facet |
glycomics norovirus ligands oysters methylation |
description |
Noroviruses, the major cause of acute viral gastroenteritis, are known to bind to histo-blood group antigens (HBGAs), including ABH groups and Lewis-type epitopes, which decorate the surface of erythrocytes and epithelial cells of their host tissues. The biosynthesis of these antigens is controlled by several glycosyltransferases, the distribution and expression of which varies between tissues and individuals. The use of HBGAs as ligands by viruses is not limited to humans, as many animal species, including oysters, which synthesize similar glycan epitopes that act as a gateway for viruses, become vectors for viral infection in humans. Here, we show that different oyster species synthesize a wide range of N-glycans that share histo-blood A-antigens but differ in the expression of other terminal antigens and in their modification by O-methyl groups. In particular, we show that the N-glycans isolated from Crassostrea gigas and Ostrea edulis exhibit exquisite methylation patterns in their terminal N-acetylgalactosamine and fucose residues in terms of position and number, adding another layer of complexity to the post-translational glycosylation modifications of glycoproteins. Furthermore, modeling of the interactions between norovirus capsid proteins and carbohydrate ligands strongly suggests that methylation has the potential to fine-tune the recognition events of oysters by virus particles. |
format |
Article in Journal/Newspaper |
author |
Auger, Audrey Yu, Shin-yi Guu, Shih-yun Quéméner, Agnès Euller, Gabriel Ando, Hiromune Desdouits, Marion Le Guyader, Soizick Khoo, Kay-hooi Le Pendu, Jacques Chirat, Frédéric Guerardel, Yann |
author_facet |
Auger, Audrey Yu, Shin-yi Guu, Shih-yun Quéméner, Agnès Euller, Gabriel Ando, Hiromune Desdouits, Marion Le Guyader, Soizick Khoo, Kay-hooi Le Pendu, Jacques Chirat, Frédéric Guerardel, Yann |
author_sort |
Auger, Audrey |
title |
Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_short |
Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_full |
Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_fullStr |
Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_full_unstemmed |
Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_sort |
species-specific n-glycomes and methylation patterns of oysters crassostrea gigas and ostrea edulis and their possible consequences for the norovirus–hbga interaction |
publisher |
MDPI |
publishDate |
2023 |
url |
https://archimer.ifremer.fr/doc/00840/95215/102930.pdf https://archimer.ifremer.fr/doc/00840/95215/102931.zip https://archimer.ifremer.fr/doc/00840/95215/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Marine Drugs (1660-3397) (MDPI), 2023-06 , Vol. 21 , N. 6 , P. 342 (25p.) |
op_relation |
https://archimer.ifremer.fr/doc/00840/95215/102930.pdf https://archimer.ifremer.fr/doc/00840/95215/102931.zip https://archimer.ifremer.fr/doc/00840/95215/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
_version_ |
1774716952799870976 |