Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas.
Early life stages are crucial for organism development, especially for those displaying external fertilization, whose gametes and early stages face environmental stressors such as xenobiotics. The pacific oyster, Crassostrea gigas, is considered a model species in ecotoxicology because of its ecolog...
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Online Access: | https://archimer.ifremer.fr/doc/00829/94090/101184.pdf https://doi.org/10.1016/j.envpol.2023.121472 https://archimer.ifremer.fr/doc/00829/94090/ |
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ftarchimer:oai:archimer.ifremer.fr:94090 2024-04-21T08:00:39+00:00 Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas. Sol Dourdin, Thomas Rivière, Guillaume Cormier, Alexandre Di Poi Broussard, Carole Guyomard, Killian Rabiller, Manuella Akcha, Farida Bah, Thierno Sadialiou Le Monier, Pauline Sussarellu, Rossana 2023-06 application/pdf https://archimer.ifremer.fr/doc/00829/94090/101184.pdf https://doi.org/10.1016/j.envpol.2023.121472 https://archimer.ifremer.fr/doc/00829/94090/ eng eng Elsevier BV https://archimer.ifremer.fr/doc/00829/94090/101184.pdf doi:10.1016/j.envpol.2023.121472 https://archimer.ifremer.fr/doc/00829/94090/ info:eu-repo/semantics/openAccess restricted use Environmental Pollution (0269-7491) (Elsevier BV), 2023-06 , Vol. 326 , P. 121472 (11p.) Mixture Epigenetics Gene expression Molluscs Pesticides DNA methylation text Article info:eu-repo/semantics/article 2023 ftarchimer https://doi.org/10.1016/j.envpol.2023.121472 2024-03-27T15:28:15Z Early life stages are crucial for organism development, especially for those displaying external fertilization, whose gametes and early stages face environmental stressors such as xenobiotics. The pacific oyster, Crassostrea gigas, is considered a model species in ecotoxicology because of its ecological characteristics (benthic, sessile, filter feeding). So far studies have investigated the impact of xenobiotics at embryotoxic, genotoxic and physiological endpoints, sometimes at the multigeneration scale, highlighting the role of epigenetic mechanisms in transmitting alterations induced by exposure to single xenobiotics. However, to date, little is known about the impact of environmentally-mimicking contaminants cocktails. Thus, we examined the impact of an early exposure to environmentally relevant mixture on the Pacific oyster life history. We studied transcriptomic, epigenetic and physiological alterations induced in oysters exposed to 18 pesticides and metals at environmental concentration (nominal sum concentration: 2.85 μg.L−1, measured sum concentration: 3.74 ± 0.013 μg.L−1) during embryo-larval stage (0–48 h post fertilization, hpf). No significant differences in embryo-larval abnormalities at 24 hpf were observed during larval and spat rearing; the swimming behaviour of exposed individuals was disturbed, while they were longer and heavier at specific time points, and exhibited a lower epinephrine-induced metamorphosis rate as well as a higher survival rate in the field. In addition, RNA-seq analyses of gastrula embryos revealed the differential expression of development-related genes (e.g. Hox orthologues and cell cycle regulators) between control and exposed oysters. Whole-genome DNA methylation analyses demonstrated a significant modification of DNA methylation in exposed larvae marked by a demethylation trend. Those findings suggest that early exposure to an environmentally relevant pesticide mixture induces multi-scale latent effects possibly affecting life history traits in the Pacific oyster. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Environmental Pollution 326 121472 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Mixture Epigenetics Gene expression Molluscs Pesticides DNA methylation |
spellingShingle |
Mixture Epigenetics Gene expression Molluscs Pesticides DNA methylation Sol Dourdin, Thomas Rivière, Guillaume Cormier, Alexandre Di Poi Broussard, Carole Guyomard, Killian Rabiller, Manuella Akcha, Farida Bah, Thierno Sadialiou Le Monier, Pauline Sussarellu, Rossana Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas. |
topic_facet |
Mixture Epigenetics Gene expression Molluscs Pesticides DNA methylation |
description |
Early life stages are crucial for organism development, especially for those displaying external fertilization, whose gametes and early stages face environmental stressors such as xenobiotics. The pacific oyster, Crassostrea gigas, is considered a model species in ecotoxicology because of its ecological characteristics (benthic, sessile, filter feeding). So far studies have investigated the impact of xenobiotics at embryotoxic, genotoxic and physiological endpoints, sometimes at the multigeneration scale, highlighting the role of epigenetic mechanisms in transmitting alterations induced by exposure to single xenobiotics. However, to date, little is known about the impact of environmentally-mimicking contaminants cocktails. Thus, we examined the impact of an early exposure to environmentally relevant mixture on the Pacific oyster life history. We studied transcriptomic, epigenetic and physiological alterations induced in oysters exposed to 18 pesticides and metals at environmental concentration (nominal sum concentration: 2.85 μg.L−1, measured sum concentration: 3.74 ± 0.013 μg.L−1) during embryo-larval stage (0–48 h post fertilization, hpf). No significant differences in embryo-larval abnormalities at 24 hpf were observed during larval and spat rearing; the swimming behaviour of exposed individuals was disturbed, while they were longer and heavier at specific time points, and exhibited a lower epinephrine-induced metamorphosis rate as well as a higher survival rate in the field. In addition, RNA-seq analyses of gastrula embryos revealed the differential expression of development-related genes (e.g. Hox orthologues and cell cycle regulators) between control and exposed oysters. Whole-genome DNA methylation analyses demonstrated a significant modification of DNA methylation in exposed larvae marked by a demethylation trend. Those findings suggest that early exposure to an environmentally relevant pesticide mixture induces multi-scale latent effects possibly affecting life history traits in the Pacific oyster. |
format |
Article in Journal/Newspaper |
author |
Sol Dourdin, Thomas Rivière, Guillaume Cormier, Alexandre Di Poi Broussard, Carole Guyomard, Killian Rabiller, Manuella Akcha, Farida Bah, Thierno Sadialiou Le Monier, Pauline Sussarellu, Rossana |
author_facet |
Sol Dourdin, Thomas Rivière, Guillaume Cormier, Alexandre Di Poi Broussard, Carole Guyomard, Killian Rabiller, Manuella Akcha, Farida Bah, Thierno Sadialiou Le Monier, Pauline Sussarellu, Rossana |
author_sort |
Sol Dourdin, Thomas |
title |
Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas. |
title_short |
Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas. |
title_full |
Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas. |
title_fullStr |
Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas. |
title_full_unstemmed |
Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas. |
title_sort |
molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the pacific oyster, crassostrea gigas. |
publisher |
Elsevier BV |
publishDate |
2023 |
url |
https://archimer.ifremer.fr/doc/00829/94090/101184.pdf https://doi.org/10.1016/j.envpol.2023.121472 https://archimer.ifremer.fr/doc/00829/94090/ |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Environmental Pollution (0269-7491) (Elsevier BV), 2023-06 , Vol. 326 , P. 121472 (11p.) |
op_relation |
https://archimer.ifremer.fr/doc/00829/94090/101184.pdf doi:10.1016/j.envpol.2023.121472 https://archimer.ifremer.fr/doc/00829/94090/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.envpol.2023.121472 |
container_title |
Environmental Pollution |
container_volume |
326 |
container_start_page |
121472 |
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1796941109268578304 |