Screening of marine lactic acid bacteria for Vibrio parahaemolyticus inhibition and application to depuration in Pacific oysters (Crassostrea gigas)
Aims This study aims to assess the use of marine lactic acid bacteria (LAB) to reduce V. parahaemolyticus levels during oyster depuration process. Methods and Results The inhibitory effect of thirty marine LAB strains against V. parahaemolyticus strains was evaluated by in vitro assays. Three positi...
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Wiley / Blackwell
2023
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Online Access: | https://archimer.ifremer.fr/doc/00813/92449/98654.pdf https://doi.org/10.1093/jambio/lxac081 https://archimer.ifremer.fr/doc/00813/92449/ |
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ftarchimer:oai:archimer.ifremer.fr:92449 2024-01-14T10:06:16+01:00 Screening of marine lactic acid bacteria for Vibrio parahaemolyticus inhibition and application to depuration in Pacific oysters (Crassostrea gigas) Soree, Marion Kolypczuk, Laetitia Hadjiev, Emilie Lozach, Solen Verrez-bagnis, Véronique Delbarre-ladrat, Christine Hervio Heath, Dominique Passerini, Delphine 2023-02 application/pdf https://archimer.ifremer.fr/doc/00813/92449/98654.pdf https://doi.org/10.1093/jambio/lxac081 https://archimer.ifremer.fr/doc/00813/92449/ eng eng Wiley / Blackwell https://archimer.ifremer.fr/doc/00813/92449/98654.pdf doi:10.1093/jambio/lxac081 https://archimer.ifremer.fr/doc/00813/92449/ info:eu-repo/semantics/openAccess restricted use Journal of Applied Microbiology (1364-5072) (Wiley / Blackwell), 2023-02 , Vol. 134 , N. 2 , P. 1-9 V. parahaemolyticus Lactic acid bacteria flow cytometry oyster depuration challenge test seafood safety text Article info:eu-repo/semantics/article 2023 ftarchimer https://doi.org/10.1093/jambio/lxac081 2023-12-19T23:51:09Z Aims This study aims to assess the use of marine lactic acid bacteria (LAB) to reduce V. parahaemolyticus levels during oyster depuration process. Methods and Results The inhibitory effect of thirty marine LAB strains against V. parahaemolyticus strains was evaluated by in vitro assays. Three positive strains (Latilactobacillus sakei SF1583, Lactococcus lactis SF1945 and Vagococcus fluvialis CD264) were selected for V. parahaemolyticus levels reduction during oyster depuration. Pacific oysters Crassostrea gigas were artificially and independently contaminated by four GFP-labelled V. parahaemolyticus strains (IFVp201, IFVp69, IFVp195 and LMG2850T) at 105 CFU.mL−1 and then exposed by balneation to 106 CFU.mL−1 of each LAB strains during 24 h, at 19°C. Quantification of V. parahaemolyticus in haemolymph by flow cytometry revealed variations in natural depuration of the different V. parahaemolyticus strains alone. Furthermore, the addition of LABs improved up to 1-log bacteria.mL−1 the reduction of IFVp201 concentration in comparison to the control condition. Conclusions Although further optimizations of procedure are needed, addition of marine LABs during oyster depuration may be an interesting strategy to reduce V. parahaemolyticus levels in C. gigas. Significance and impact of the study Our study provides promising ways to develop a depuration process which could potentially be implemented in oyster farms. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Journal of Applied Microbiology 134 2 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
V. parahaemolyticus Lactic acid bacteria flow cytometry oyster depuration challenge test seafood safety |
spellingShingle |
V. parahaemolyticus Lactic acid bacteria flow cytometry oyster depuration challenge test seafood safety Soree, Marion Kolypczuk, Laetitia Hadjiev, Emilie Lozach, Solen Verrez-bagnis, Véronique Delbarre-ladrat, Christine Hervio Heath, Dominique Passerini, Delphine Screening of marine lactic acid bacteria for Vibrio parahaemolyticus inhibition and application to depuration in Pacific oysters (Crassostrea gigas) |
topic_facet |
V. parahaemolyticus Lactic acid bacteria flow cytometry oyster depuration challenge test seafood safety |
description |
Aims This study aims to assess the use of marine lactic acid bacteria (LAB) to reduce V. parahaemolyticus levels during oyster depuration process. Methods and Results The inhibitory effect of thirty marine LAB strains against V. parahaemolyticus strains was evaluated by in vitro assays. Three positive strains (Latilactobacillus sakei SF1583, Lactococcus lactis SF1945 and Vagococcus fluvialis CD264) were selected for V. parahaemolyticus levels reduction during oyster depuration. Pacific oysters Crassostrea gigas were artificially and independently contaminated by four GFP-labelled V. parahaemolyticus strains (IFVp201, IFVp69, IFVp195 and LMG2850T) at 105 CFU.mL−1 and then exposed by balneation to 106 CFU.mL−1 of each LAB strains during 24 h, at 19°C. Quantification of V. parahaemolyticus in haemolymph by flow cytometry revealed variations in natural depuration of the different V. parahaemolyticus strains alone. Furthermore, the addition of LABs improved up to 1-log bacteria.mL−1 the reduction of IFVp201 concentration in comparison to the control condition. Conclusions Although further optimizations of procedure are needed, addition of marine LABs during oyster depuration may be an interesting strategy to reduce V. parahaemolyticus levels in C. gigas. Significance and impact of the study Our study provides promising ways to develop a depuration process which could potentially be implemented in oyster farms. |
format |
Article in Journal/Newspaper |
author |
Soree, Marion Kolypczuk, Laetitia Hadjiev, Emilie Lozach, Solen Verrez-bagnis, Véronique Delbarre-ladrat, Christine Hervio Heath, Dominique Passerini, Delphine |
author_facet |
Soree, Marion Kolypczuk, Laetitia Hadjiev, Emilie Lozach, Solen Verrez-bagnis, Véronique Delbarre-ladrat, Christine Hervio Heath, Dominique Passerini, Delphine |
author_sort |
Soree, Marion |
title |
Screening of marine lactic acid bacteria for Vibrio parahaemolyticus inhibition and application to depuration in Pacific oysters (Crassostrea gigas) |
title_short |
Screening of marine lactic acid bacteria for Vibrio parahaemolyticus inhibition and application to depuration in Pacific oysters (Crassostrea gigas) |
title_full |
Screening of marine lactic acid bacteria for Vibrio parahaemolyticus inhibition and application to depuration in Pacific oysters (Crassostrea gigas) |
title_fullStr |
Screening of marine lactic acid bacteria for Vibrio parahaemolyticus inhibition and application to depuration in Pacific oysters (Crassostrea gigas) |
title_full_unstemmed |
Screening of marine lactic acid bacteria for Vibrio parahaemolyticus inhibition and application to depuration in Pacific oysters (Crassostrea gigas) |
title_sort |
screening of marine lactic acid bacteria for vibrio parahaemolyticus inhibition and application to depuration in pacific oysters (crassostrea gigas) |
publisher |
Wiley / Blackwell |
publishDate |
2023 |
url |
https://archimer.ifremer.fr/doc/00813/92449/98654.pdf https://doi.org/10.1093/jambio/lxac081 https://archimer.ifremer.fr/doc/00813/92449/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Journal of Applied Microbiology (1364-5072) (Wiley / Blackwell), 2023-02 , Vol. 134 , N. 2 , P. 1-9 |
op_relation |
https://archimer.ifremer.fr/doc/00813/92449/98654.pdf doi:10.1093/jambio/lxac081 https://archimer.ifremer.fr/doc/00813/92449/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1093/jambio/lxac081 |
container_title |
Journal of Applied Microbiology |
container_volume |
134 |
container_issue |
2 |
_version_ |
1788060575885950976 |