Functional Diversification of Oyster Big Defensins Generates Antimicrobial Specificity and Synergy against Members of the Microbiota
Big defensins are two-domain antimicrobial peptides (AMPs) that have highly diversified in mollusks. Cg-BigDefs are expressed by immune cells in the oyster Crassostrea gigas, and their expression is dampened during the Pacific Oyster Mortality Syndrome (POMS), which evolves toward fatal bacteremia....
Published in: | Marine Drugs |
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Format: | Article in Journal/Newspaper |
Language: | English |
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MDPI AG
2022
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Online Access: | https://archimer.ifremer.fr/doc/00807/91932/97892.pdf https://archimer.ifremer.fr/doc/00807/91932/97893.zip https://doi.org/10.3390/md20120745 https://archimer.ifremer.fr/doc/00807/91932/ |
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ftarchimer:oai:archimer.ifremer.fr:91932 2023-05-15T15:58:57+02:00 Functional Diversification of Oyster Big Defensins Generates Antimicrobial Specificity and Synergy against Members of the Microbiota De San Nicolas, Noémie Asokan, Aromal Rosa, Rafael D. Voisin, Sébastien N. Travers, Agnes Rocha, Gustavo Dantan, Luc Dorant, Yann Mitta, Guillaume Petton, Bruno Charrière, Guillaume M. Escoubas, Jean Michel Boulo, Viviane Pouzadoux, Juliette Meudal, Hervé Loth, Karine Aucagne, Vincent Delmas, Agnès F. Bulet, Philippe Montagnani, Caroline Destoumieux Garzon, Delphine 2022-12 application/pdf https://archimer.ifremer.fr/doc/00807/91932/97892.pdf https://archimer.ifremer.fr/doc/00807/91932/97893.zip https://doi.org/10.3390/md20120745 https://archimer.ifremer.fr/doc/00807/91932/ eng eng MDPI AG https://archimer.ifremer.fr/doc/00807/91932/97892.pdf https://archimer.ifremer.fr/doc/00807/91932/97893.zip doi:10.3390/md20120745 https://archimer.ifremer.fr/doc/00807/91932/ info:eu-repo/semantics/openAccess restricted use Marine Drugs (1660-3397) (MDPI AG), 2022-12 , Vol. 20 , N. 12 , P. 745 (18p.) antibacterial peptide beta defensin diversity evolution microbiome invertebrate mollusk text Publication info:eu-repo/semantics/article 2022 ftarchimer https://doi.org/10.3390/md20120745 2023-01-24T23:50:40Z Big defensins are two-domain antimicrobial peptides (AMPs) that have highly diversified in mollusks. Cg-BigDefs are expressed by immune cells in the oyster Crassostrea gigas, and their expression is dampened during the Pacific Oyster Mortality Syndrome (POMS), which evolves toward fatal bacteremia. We evaluated whether Cg-BigDefs contribute to the control of oyster-associated microbial communities. Two Cg-BigDefs that are representative of molecular diversity within the peptide family, namely Cg-BigDef1 and Cg-BigDef5, were characterized by gene cloning and synthesized by solid-phase peptide synthesis and native chemical ligation. Synthetic peptides were tested for antibacterial activity against a collection of culturable bacteria belonging to the oyster microbiota, characterized by 16S sequencing and MALDI Biotyping. We first tested the potential of Cg-BigDefs to control the oyster microbiota by injecting synthetic Cg-BigDef1 into oyster tissues and analyzing microbiota dynamics over 24 h by 16S metabarcoding. Cg-BigDef1 induced a significant shift in oyster microbiota β-diversity after 6 h and 24 h, prompting us to investigate antimicrobial activities in vitro against members of the oyster microbiota. Both Cg-BigDef1 and Cg-BigDef5 were active at a high salt concentration (400 mM NaCl) and showed broad spectra of activity against bacteria associated with C. gigas pathologies. Antimicrobial specificity was observed for both molecules at an intra- and inter-genera level. Remarkably, antimicrobial spectra of Cg-BigDef1 and Cg-BigDef5 were complementary, and peptides acted synergistically. Overall, we found that primary sequence diversification of Cg-BigDefs has generated specificity and synergy and extended the spectrum of activity of this peptide family. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Marine Drugs 20 12 745 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
antibacterial peptide beta defensin diversity evolution microbiome invertebrate mollusk |
spellingShingle |
antibacterial peptide beta defensin diversity evolution microbiome invertebrate mollusk De San Nicolas, Noémie Asokan, Aromal Rosa, Rafael D. Voisin, Sébastien N. Travers, Agnes Rocha, Gustavo Dantan, Luc Dorant, Yann Mitta, Guillaume Petton, Bruno Charrière, Guillaume M. Escoubas, Jean Michel Boulo, Viviane Pouzadoux, Juliette Meudal, Hervé Loth, Karine Aucagne, Vincent Delmas, Agnès F. Bulet, Philippe Montagnani, Caroline Destoumieux Garzon, Delphine Functional Diversification of Oyster Big Defensins Generates Antimicrobial Specificity and Synergy against Members of the Microbiota |
topic_facet |
antibacterial peptide beta defensin diversity evolution microbiome invertebrate mollusk |
description |
Big defensins are two-domain antimicrobial peptides (AMPs) that have highly diversified in mollusks. Cg-BigDefs are expressed by immune cells in the oyster Crassostrea gigas, and their expression is dampened during the Pacific Oyster Mortality Syndrome (POMS), which evolves toward fatal bacteremia. We evaluated whether Cg-BigDefs contribute to the control of oyster-associated microbial communities. Two Cg-BigDefs that are representative of molecular diversity within the peptide family, namely Cg-BigDef1 and Cg-BigDef5, were characterized by gene cloning and synthesized by solid-phase peptide synthesis and native chemical ligation. Synthetic peptides were tested for antibacterial activity against a collection of culturable bacteria belonging to the oyster microbiota, characterized by 16S sequencing and MALDI Biotyping. We first tested the potential of Cg-BigDefs to control the oyster microbiota by injecting synthetic Cg-BigDef1 into oyster tissues and analyzing microbiota dynamics over 24 h by 16S metabarcoding. Cg-BigDef1 induced a significant shift in oyster microbiota β-diversity after 6 h and 24 h, prompting us to investigate antimicrobial activities in vitro against members of the oyster microbiota. Both Cg-BigDef1 and Cg-BigDef5 were active at a high salt concentration (400 mM NaCl) and showed broad spectra of activity against bacteria associated with C. gigas pathologies. Antimicrobial specificity was observed for both molecules at an intra- and inter-genera level. Remarkably, antimicrobial spectra of Cg-BigDef1 and Cg-BigDef5 were complementary, and peptides acted synergistically. Overall, we found that primary sequence diversification of Cg-BigDefs has generated specificity and synergy and extended the spectrum of activity of this peptide family. |
format |
Article in Journal/Newspaper |
author |
De San Nicolas, Noémie Asokan, Aromal Rosa, Rafael D. Voisin, Sébastien N. Travers, Agnes Rocha, Gustavo Dantan, Luc Dorant, Yann Mitta, Guillaume Petton, Bruno Charrière, Guillaume M. Escoubas, Jean Michel Boulo, Viviane Pouzadoux, Juliette Meudal, Hervé Loth, Karine Aucagne, Vincent Delmas, Agnès F. Bulet, Philippe Montagnani, Caroline Destoumieux Garzon, Delphine |
author_facet |
De San Nicolas, Noémie Asokan, Aromal Rosa, Rafael D. Voisin, Sébastien N. Travers, Agnes Rocha, Gustavo Dantan, Luc Dorant, Yann Mitta, Guillaume Petton, Bruno Charrière, Guillaume M. Escoubas, Jean Michel Boulo, Viviane Pouzadoux, Juliette Meudal, Hervé Loth, Karine Aucagne, Vincent Delmas, Agnès F. Bulet, Philippe Montagnani, Caroline Destoumieux Garzon, Delphine |
author_sort |
De San Nicolas, Noémie |
title |
Functional Diversification of Oyster Big Defensins Generates Antimicrobial Specificity and Synergy against Members of the Microbiota |
title_short |
Functional Diversification of Oyster Big Defensins Generates Antimicrobial Specificity and Synergy against Members of the Microbiota |
title_full |
Functional Diversification of Oyster Big Defensins Generates Antimicrobial Specificity and Synergy against Members of the Microbiota |
title_fullStr |
Functional Diversification of Oyster Big Defensins Generates Antimicrobial Specificity and Synergy against Members of the Microbiota |
title_full_unstemmed |
Functional Diversification of Oyster Big Defensins Generates Antimicrobial Specificity and Synergy against Members of the Microbiota |
title_sort |
functional diversification of oyster big defensins generates antimicrobial specificity and synergy against members of the microbiota |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://archimer.ifremer.fr/doc/00807/91932/97892.pdf https://archimer.ifremer.fr/doc/00807/91932/97893.zip https://doi.org/10.3390/md20120745 https://archimer.ifremer.fr/doc/00807/91932/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Marine Drugs (1660-3397) (MDPI AG), 2022-12 , Vol. 20 , N. 12 , P. 745 (18p.) |
op_relation |
https://archimer.ifremer.fr/doc/00807/91932/97892.pdf https://archimer.ifremer.fr/doc/00807/91932/97893.zip doi:10.3390/md20120745 https://archimer.ifremer.fr/doc/00807/91932/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.3390/md20120745 |
container_title |
Marine Drugs |
container_volume |
20 |
container_issue |
12 |
container_start_page |
745 |
_version_ |
1766394729112535040 |