Understanding the Dynamic of POMS Infection and the Role of Microbiota Composition in the Survival of Pacific Oysters, Crassostrea gigas

• Published in: Microbiology Spectrum
• Main Authors: Delisle, Lizenn, Laroche, Olivier, Hilton, Zoë, Burguin, Jean-françois, Rolton, Anne, Berry, Jolene, Pochon, Xavier, Boudry, Pierre, Vignier, Julien
• Format: Article in Journal/Newspaper
• Language: English
• Published: American Society for Microbiology 2022
• Subjects: OsHV-1; Pacific oyster; POMS; microbiome; 16S rRNA gene sequencing; droplet digital PCR; Pacific; Crassostrea gigas
• Online Access: https://archimer.ifremer.fr/doc/00807/91870/97819.pdf; https://archimer.ifremer.fr/doc/00807/91870/97820.pdf; https://archimer.ifremer.fr/doc/00807/91870/97821.xlsx; https://archimer.ifremer.fr/doc/00807/91870/97822.xlsx; https://archimer.ifremer.fr/doc/00807/91870/97823.xlsx; https://doi.org/10.1128/spectrum.01959-22; https://archimer.ifremer.fr/doc/00807/91870/

For over a decade, Pacific oyster mortality syndrome (POMS), a polymicrobial disease, induced recurring episodes of massive mortality affecting Crassostrea gigas oysters worldwide. Recent studies evidenced a combined infection of the ostreid herpesvirus (OsHV-1 μVar) and opportunistic bacteria in affected oysters. However, the role of the oyster microbiota in POMS is not fully understood. While some bacteria can protect hosts from infection, even minor changes to the microbial communities may also facilitate infection and worsen disease severity. Using a laboratory-based experimental infection model, we challenged juveniles from 10 biparental oyster families with previously established contrasted genetically based ability to survive POMS in the field. Combining molecular analyses and 16S rRNA gene sequencing with histopathological observations, we described the temporal kinetics of POMS and characterized the changes in microbiota during infection. By associating the microbiota composition with oyster mortality rate, viral load, and viral gene expression, we were able to identify both potentially harmful and beneficial bacterial amplicon sequence variants (ASVs). We also observed a delay in viral infection resulting in a later onset of mortality in oysters compared to previous observations and a lack of evidence of fatal dysbiosis in infected oysters. Overall, these results provide new insights into how the oyster microbiome may influence POMS disease outcomes and open new perspectives on the use of microbiome composition as a complementary screening tool to determine shellfish health and potentially predict oyster vulnerability to POMS. IMPORTANCE For more than a decade, Pacific oyster mortality syndrome (POMS) has severely impacted the Crassostrea gigas aquaculture industry, at times killing up to 100% of young farmed Pacific oysters, a key commercial species that is cultivated globally. These disease outbreaks have caused major financial losses for the oyster aquaculture industry. Selective breeding has ...