Tetrodotoxins in French Bivalve Mollusks—Analytical Methodology, Environmental Dynamics and Screening of Bacterial Strain Collections
Tetrodotoxins (TTXs) are potentially lethal paralytic toxins that have been identified in European shellfish over recent years. Risk assessment has suggested comparatively low levels (44 µg TTX-equivalent/kg) but stresses the lack of data on occurrence. Both bacteria and dinoflagellates were suggest...
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ftarchimer:oai:archimer.ifremer.fr:84205 2023-05-15T15:58:46+02:00 Tetrodotoxins in French Bivalve Mollusks—Analytical Methodology, Environmental Dynamics and Screening of Bacterial Strain Collections Réveillon, Damien Savar, Veronique Schaefer, Estelle Chevé, Julien Halm-lemeille, Marie-pierre Hervio-heath, Dominique Travers, Marie-agnes Abadie, Eric Rolland, Jean-luc Hess, Philipp 2021-11 application/pdf https://archimer.ifremer.fr/doc/00730/84205/89130.pdf https://archimer.ifremer.fr/doc/00730/84205/89131.zip https://doi.org/10.3390/toxins13110740 https://archimer.ifremer.fr/doc/00730/84205/ eng eng MDPI AG https://archimer.ifremer.fr/doc/00730/84205/89130.pdf https://archimer.ifremer.fr/doc/00730/84205/89131.zip doi:10.3390/toxins13110740 https://archimer.ifremer.fr/doc/00730/84205/ info:eu-repo/semantics/openAccess restricted use Toxins (2072-6651) (MDPI AG), 2021-11 , Vol. 13 , N. 11 , P. 740 (18p.) emerging toxins TTXs REPHY REMI coastal and seafood contamination text Publication info:eu-repo/semantics/article 2021 ftarchimer https://doi.org/10.3390/toxins13110740 2021-12-21T23:50:15Z Tetrodotoxins (TTXs) are potentially lethal paralytic toxins that have been identified in European shellfish over recent years. Risk assessment has suggested comparatively low levels (44 µg TTX-equivalent/kg) but stresses the lack of data on occurrence. Both bacteria and dinoflagellates were suggested as possible biogenic sources, either from an endogenous or exogenous origin. We thus investigated TTXs in (i) 98 shellfish samples and (ii) 122 bacterial strains, isolated from French environments. We optimized a method based on mass spectrometry, using a single extraction step followed by ultrafiltration without Solid Phase Extraction and matrix-matched calibration for both shellfish and bacterial matrix. Limits of detection and quantification were 6.3 and 12.5 µg/kg for shellfish and 5.0 and 10 µg/kg for bacterial matrix, respectively. Even though bacterial matrix resulted in signal enhancement, no TTX analog was detected in any strain. Bivalves (either Crassostrea gigas or Ruditapes philippinarum) were surveyed in six French production areas over 2.5–3 month periods (2018–2019). Concentrations of TTX ranged from ‘not detected’ to a maximum of 32 µg/kg (Bay of Brest, 17 June 2019), with events lasting 2 weeks at maximum. While these results are in line with previous studies, they provide new data of TTX occurrence and confirm that the link between bacteria, bivalves and TTX is complex Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Toxins 13 11 740 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
emerging toxins TTXs REPHY REMI coastal and seafood contamination |
spellingShingle |
emerging toxins TTXs REPHY REMI coastal and seafood contamination Réveillon, Damien Savar, Veronique Schaefer, Estelle Chevé, Julien Halm-lemeille, Marie-pierre Hervio-heath, Dominique Travers, Marie-agnes Abadie, Eric Rolland, Jean-luc Hess, Philipp Tetrodotoxins in French Bivalve Mollusks—Analytical Methodology, Environmental Dynamics and Screening of Bacterial Strain Collections |
topic_facet |
emerging toxins TTXs REPHY REMI coastal and seafood contamination |
description |
Tetrodotoxins (TTXs) are potentially lethal paralytic toxins that have been identified in European shellfish over recent years. Risk assessment has suggested comparatively low levels (44 µg TTX-equivalent/kg) but stresses the lack of data on occurrence. Both bacteria and dinoflagellates were suggested as possible biogenic sources, either from an endogenous or exogenous origin. We thus investigated TTXs in (i) 98 shellfish samples and (ii) 122 bacterial strains, isolated from French environments. We optimized a method based on mass spectrometry, using a single extraction step followed by ultrafiltration without Solid Phase Extraction and matrix-matched calibration for both shellfish and bacterial matrix. Limits of detection and quantification were 6.3 and 12.5 µg/kg for shellfish and 5.0 and 10 µg/kg for bacterial matrix, respectively. Even though bacterial matrix resulted in signal enhancement, no TTX analog was detected in any strain. Bivalves (either Crassostrea gigas or Ruditapes philippinarum) were surveyed in six French production areas over 2.5–3 month periods (2018–2019). Concentrations of TTX ranged from ‘not detected’ to a maximum of 32 µg/kg (Bay of Brest, 17 June 2019), with events lasting 2 weeks at maximum. While these results are in line with previous studies, they provide new data of TTX occurrence and confirm that the link between bacteria, bivalves and TTX is complex |
format |
Article in Journal/Newspaper |
author |
Réveillon, Damien Savar, Veronique Schaefer, Estelle Chevé, Julien Halm-lemeille, Marie-pierre Hervio-heath, Dominique Travers, Marie-agnes Abadie, Eric Rolland, Jean-luc Hess, Philipp |
author_facet |
Réveillon, Damien Savar, Veronique Schaefer, Estelle Chevé, Julien Halm-lemeille, Marie-pierre Hervio-heath, Dominique Travers, Marie-agnes Abadie, Eric Rolland, Jean-luc Hess, Philipp |
author_sort |
Réveillon, Damien |
title |
Tetrodotoxins in French Bivalve Mollusks—Analytical Methodology, Environmental Dynamics and Screening of Bacterial Strain Collections |
title_short |
Tetrodotoxins in French Bivalve Mollusks—Analytical Methodology, Environmental Dynamics and Screening of Bacterial Strain Collections |
title_full |
Tetrodotoxins in French Bivalve Mollusks—Analytical Methodology, Environmental Dynamics and Screening of Bacterial Strain Collections |
title_fullStr |
Tetrodotoxins in French Bivalve Mollusks—Analytical Methodology, Environmental Dynamics and Screening of Bacterial Strain Collections |
title_full_unstemmed |
Tetrodotoxins in French Bivalve Mollusks—Analytical Methodology, Environmental Dynamics and Screening of Bacterial Strain Collections |
title_sort |
tetrodotoxins in french bivalve mollusks—analytical methodology, environmental dynamics and screening of bacterial strain collections |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://archimer.ifremer.fr/doc/00730/84205/89130.pdf https://archimer.ifremer.fr/doc/00730/84205/89131.zip https://doi.org/10.3390/toxins13110740 https://archimer.ifremer.fr/doc/00730/84205/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Toxins (2072-6651) (MDPI AG), 2021-11 , Vol. 13 , N. 11 , P. 740 (18p.) |
op_relation |
https://archimer.ifremer.fr/doc/00730/84205/89130.pdf https://archimer.ifremer.fr/doc/00730/84205/89131.zip doi:10.3390/toxins13110740 https://archimer.ifremer.fr/doc/00730/84205/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.3390/toxins13110740 |
container_title |
Toxins |
container_volume |
13 |
container_issue |
11 |
container_start_page |
740 |
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1766394545405165568 |