The toxic dinoflagellate Alexandrium minutum affects oyster gamete health and fertilization potential
Dinoflagellates from the globally distributed genus Alexandrium are known to produce both paralytic shellfish toxins (PST) and uncharacterized bioactive extracellular compounds (BEC) with allelopathic, ichthyotoxic, hemolytic and cytotoxic activities. In France, blooms of Alexandrium minutum appear...
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ftarchimer:oai:archimer.ifremer.fr:81322 2023-05-15T17:54:20+02:00 The toxic dinoflagellate Alexandrium minutum affects oyster gamete health and fertilization potential Castrec, Justine Fabioux, Caroline Le Goïc, Nelly Boulais, Myrina Soudant, Philippe Hégaret, Helene 2021-07 application/pdf https://archimer.ifremer.fr/doc/00701/81322/85687.pdf https://doi.org/10.1016/j.marenvres.2021.105401 https://archimer.ifremer.fr/doc/00701/81322/ eng eng Elsevier BV https://archimer.ifremer.fr/doc/00701/81322/85687.pdf doi:10.1016/j.marenvres.2021.105401 https://archimer.ifremer.fr/doc/00701/81322/ info:eu-repo/semantics/openAccess restricted use Marine Environmental Research (0141-1136) (Elsevier BV), 2021-07 , Vol. 169 , P. 105401 (7p.) Algal blooms Algal toxins Fertilization Paralytic shellfish toxins (PST) Bioactive extracellular compounds (BEC) Spermatozoa Oocyte Flow cytometry (=Magallana) gigas Bivalves text Publication info:eu-repo/semantics/article 2021 ftarchimer https://doi.org/10.1016/j.marenvres.2021.105401 2021-09-23T20:37:50Z Dinoflagellates from the globally distributed genus Alexandrium are known to produce both paralytic shellfish toxins (PST) and uncharacterized bioactive extracellular compounds (BEC) with allelopathic, ichthyotoxic, hemolytic and cytotoxic activities. In France, blooms of Alexandrium minutum appear generally during the spawning period of most bivalves. These blooms could therefore alter gametes and/or larval development of bivalves, causing severe issues for ecologically and economically important species, such as the Pacific oyster Crassostrea (=Magallana) gigas. The aim of this work was to test the effects of three strains of A. minutum producing either only PST, only BEC, or both PST and BEC upon oyster gametes, and potential consequences on fertilization success. Oocytes and spermatozoa were exposed in vitro for 2 hours to a range of environmentally realistic A. minutum concentrations (10 to 2.5 × 104 cells mL-1). Following exposure, gamete viability and reactive oxygen species (ROS) production were assessed by flow cytometry, spermatozoa motility and fertilization capacities of both spermatozoa and oocytes were analysed by microscopy. Viability and fertilization capacity of spermatozoa and oocytes were drastically reduced following exposure to 2.5 × 104 cells mL-1 of A. minutum. The BEC-producing strain was the most potent strain decreasing spermatozoa motility, increasing ROS production of oocytes, and decreasing fertilization, from the concentration of 2.5 × 103 cells mL-1. This study highlights the significant cellular toxicity of the BEC produced by A. minutum on oyster gametes. Physical contact between gametes and motile thecate A. minutum cells may also contribute to alter oyster gamete integrity. These results suggest that oyster gametes exposure to A. minutum blooms could affect oyster fertility and reproduction success. Article in Journal/Newspaper Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Marine Environmental Research 169 105401 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Algal blooms Algal toxins Fertilization Paralytic shellfish toxins (PST) Bioactive extracellular compounds (BEC) Spermatozoa Oocyte Flow cytometry (=Magallana) gigas Bivalves |
spellingShingle |
Algal blooms Algal toxins Fertilization Paralytic shellfish toxins (PST) Bioactive extracellular compounds (BEC) Spermatozoa Oocyte Flow cytometry (=Magallana) gigas Bivalves Castrec, Justine Fabioux, Caroline Le Goïc, Nelly Boulais, Myrina Soudant, Philippe Hégaret, Helene The toxic dinoflagellate Alexandrium minutum affects oyster gamete health and fertilization potential |
topic_facet |
Algal blooms Algal toxins Fertilization Paralytic shellfish toxins (PST) Bioactive extracellular compounds (BEC) Spermatozoa Oocyte Flow cytometry (=Magallana) gigas Bivalves |
description |
Dinoflagellates from the globally distributed genus Alexandrium are known to produce both paralytic shellfish toxins (PST) and uncharacterized bioactive extracellular compounds (BEC) with allelopathic, ichthyotoxic, hemolytic and cytotoxic activities. In France, blooms of Alexandrium minutum appear generally during the spawning period of most bivalves. These blooms could therefore alter gametes and/or larval development of bivalves, causing severe issues for ecologically and economically important species, such as the Pacific oyster Crassostrea (=Magallana) gigas. The aim of this work was to test the effects of three strains of A. minutum producing either only PST, only BEC, or both PST and BEC upon oyster gametes, and potential consequences on fertilization success. Oocytes and spermatozoa were exposed in vitro for 2 hours to a range of environmentally realistic A. minutum concentrations (10 to 2.5 × 104 cells mL-1). Following exposure, gamete viability and reactive oxygen species (ROS) production were assessed by flow cytometry, spermatozoa motility and fertilization capacities of both spermatozoa and oocytes were analysed by microscopy. Viability and fertilization capacity of spermatozoa and oocytes were drastically reduced following exposure to 2.5 × 104 cells mL-1 of A. minutum. The BEC-producing strain was the most potent strain decreasing spermatozoa motility, increasing ROS production of oocytes, and decreasing fertilization, from the concentration of 2.5 × 103 cells mL-1. This study highlights the significant cellular toxicity of the BEC produced by A. minutum on oyster gametes. Physical contact between gametes and motile thecate A. minutum cells may also contribute to alter oyster gamete integrity. These results suggest that oyster gametes exposure to A. minutum blooms could affect oyster fertility and reproduction success. |
format |
Article in Journal/Newspaper |
author |
Castrec, Justine Fabioux, Caroline Le Goïc, Nelly Boulais, Myrina Soudant, Philippe Hégaret, Helene |
author_facet |
Castrec, Justine Fabioux, Caroline Le Goïc, Nelly Boulais, Myrina Soudant, Philippe Hégaret, Helene |
author_sort |
Castrec, Justine |
title |
The toxic dinoflagellate Alexandrium minutum affects oyster gamete health and fertilization potential |
title_short |
The toxic dinoflagellate Alexandrium minutum affects oyster gamete health and fertilization potential |
title_full |
The toxic dinoflagellate Alexandrium minutum affects oyster gamete health and fertilization potential |
title_fullStr |
The toxic dinoflagellate Alexandrium minutum affects oyster gamete health and fertilization potential |
title_full_unstemmed |
The toxic dinoflagellate Alexandrium minutum affects oyster gamete health and fertilization potential |
title_sort |
toxic dinoflagellate alexandrium minutum affects oyster gamete health and fertilization potential |
publisher |
Elsevier BV |
publishDate |
2021 |
url |
https://archimer.ifremer.fr/doc/00701/81322/85687.pdf https://doi.org/10.1016/j.marenvres.2021.105401 https://archimer.ifremer.fr/doc/00701/81322/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_source |
Marine Environmental Research (0141-1136) (Elsevier BV), 2021-07 , Vol. 169 , P. 105401 (7p.) |
op_relation |
https://archimer.ifremer.fr/doc/00701/81322/85687.pdf doi:10.1016/j.marenvres.2021.105401 https://archimer.ifremer.fr/doc/00701/81322/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.marenvres.2021.105401 |
container_title |
Marine Environmental Research |
container_volume |
169 |
container_start_page |
105401 |
_version_ |
1766162093769228288 |