Prokaryotic diversity and activity in contrasting productivity regimes in late summer in the Kerguelen region (Southern Ocean)
Natural iron (Fe) fertilization sustains phytoplankton blooms above the Kerguelen plateau (Indian sector of the Southern Ocean) within otherwise low productive off-plateau waters. In early spring and summer, these diatom-dominated blooms are associated with distinct heterotrophic prokaryotic communi...
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2021
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Online Access: | https://archimer.ifremer.fr/doc/00694/80583/83802.pdf https://doi.org/10.1016/j.jmarsys.2021.103561 https://archimer.ifremer.fr/doc/00694/80583/ |
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ftarchimer:oai:archimer.ifremer.fr:80583 2023-09-05T13:23:28+02:00 Prokaryotic diversity and activity in contrasting productivity regimes in late summer in the Kerguelen region (Southern Ocean) Hernandez-magana, Alejandra Elisa Liu, Yan Debeljak, Pavla Crispi, Olivier Marie, Barbara Koedooder, Coco Obernosterer, Ingrid 2021-09 application/pdf https://archimer.ifremer.fr/doc/00694/80583/83802.pdf https://doi.org/10.1016/j.jmarsys.2021.103561 https://archimer.ifremer.fr/doc/00694/80583/ eng eng Elsevier BV https://archimer.ifremer.fr/doc/00694/80583/83802.pdf doi:10.1016/j.jmarsys.2021.103561 https://archimer.ifremer.fr/doc/00694/80583/ info:eu-repo/semantics/openAccess restricted use Journal Of Marine Systems (0924-7963) (Elsevier BV), 2021-09 , Vol. 221 , P. 103561 (12p.) Prokaryotic community composition 16S rRNA Amplicon sequence variants Natural iron fertilization Southern Ocean text Article info:eu-repo/semantics/article 2021 ftarchimer https://doi.org/10.1016/j.jmarsys.2021.103561 2023-08-22T22:51:07Z Natural iron (Fe) fertilization sustains phytoplankton blooms above the Kerguelen plateau (Indian sector of the Southern Ocean) within otherwise low productive off-plateau waters. In early spring and summer, these diatom-dominated blooms are associated with distinct heterotrophic prokaryotic communities, but whether a structuring effect extends to the post-bloom period has thus far not been investigated. To address this question, we carried out a detailed study of the prokaryotic community composition in the region of Kerguelen Island during late Austral summer (18 February to 27 March 2018; MOBYDICK project). Concentrations of chlorophyll a were seasonally low above the plateau (0.27–0.58 μg Chl a L−1) and in a similar range to those at the 3 off-plateau sites investigated (0.14–0.34 μg Chl a L−1), but we observed an accumulation of dissolved organic carbon and the build-up of heterotrophic prokaryotic biomass in Kerguelen plateau waters. Illumina sequencing of the 16S rRNA gene revealed that the total (DNA-based) and potentially active (RNA-based) prokaryotic communities were structured according to on- and off-plateau sites in the wind-mixed surface layer, in both the free-living (<0.8 μm size fraction) and particle-attached (>0.8 μm size fraction) size fractions. The Amplicon Sequence Variants (ASV) with significantly higher relative abundances in on-plateau surface waters as compared to off-plateau waters belonged to Halieaceae OM60 group, several Flavobacteriaceae, such as the NS5 marine group, Aurantivirga and Ulvibacter, Rhodobacteraceae Loktanella, Saprospiraceae, and the Cryomorphaceae NS10 marine group. ASVs with higher relative abundances in off-plateau waters belonged to the Flavobacteriaceae Formosa, the Rhodobacteraceae Planktomarina and the SAR11 clade. We discuss the potential abiotic and biotic drivers of community composition in late Austral summer and the ecological roles of abundant prokaryotic taxa in Kerguelen plateau waters. Article in Journal/Newspaper Southern Ocean Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Austral Indian Kerguelen Kerguelen Island ENVELOPE(69.500,69.500,-49.250,-49.250) Southern Ocean Journal of Marine Systems 221 103561 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Prokaryotic community composition 16S rRNA Amplicon sequence variants Natural iron fertilization Southern Ocean |
spellingShingle |
Prokaryotic community composition 16S rRNA Amplicon sequence variants Natural iron fertilization Southern Ocean Hernandez-magana, Alejandra Elisa Liu, Yan Debeljak, Pavla Crispi, Olivier Marie, Barbara Koedooder, Coco Obernosterer, Ingrid Prokaryotic diversity and activity in contrasting productivity regimes in late summer in the Kerguelen region (Southern Ocean) |
topic_facet |
Prokaryotic community composition 16S rRNA Amplicon sequence variants Natural iron fertilization Southern Ocean |
description |
Natural iron (Fe) fertilization sustains phytoplankton blooms above the Kerguelen plateau (Indian sector of the Southern Ocean) within otherwise low productive off-plateau waters. In early spring and summer, these diatom-dominated blooms are associated with distinct heterotrophic prokaryotic communities, but whether a structuring effect extends to the post-bloom period has thus far not been investigated. To address this question, we carried out a detailed study of the prokaryotic community composition in the region of Kerguelen Island during late Austral summer (18 February to 27 March 2018; MOBYDICK project). Concentrations of chlorophyll a were seasonally low above the plateau (0.27–0.58 μg Chl a L−1) and in a similar range to those at the 3 off-plateau sites investigated (0.14–0.34 μg Chl a L−1), but we observed an accumulation of dissolved organic carbon and the build-up of heterotrophic prokaryotic biomass in Kerguelen plateau waters. Illumina sequencing of the 16S rRNA gene revealed that the total (DNA-based) and potentially active (RNA-based) prokaryotic communities were structured according to on- and off-plateau sites in the wind-mixed surface layer, in both the free-living (<0.8 μm size fraction) and particle-attached (>0.8 μm size fraction) size fractions. The Amplicon Sequence Variants (ASV) with significantly higher relative abundances in on-plateau surface waters as compared to off-plateau waters belonged to Halieaceae OM60 group, several Flavobacteriaceae, such as the NS5 marine group, Aurantivirga and Ulvibacter, Rhodobacteraceae Loktanella, Saprospiraceae, and the Cryomorphaceae NS10 marine group. ASVs with higher relative abundances in off-plateau waters belonged to the Flavobacteriaceae Formosa, the Rhodobacteraceae Planktomarina and the SAR11 clade. We discuss the potential abiotic and biotic drivers of community composition in late Austral summer and the ecological roles of abundant prokaryotic taxa in Kerguelen plateau waters. |
format |
Article in Journal/Newspaper |
author |
Hernandez-magana, Alejandra Elisa Liu, Yan Debeljak, Pavla Crispi, Olivier Marie, Barbara Koedooder, Coco Obernosterer, Ingrid |
author_facet |
Hernandez-magana, Alejandra Elisa Liu, Yan Debeljak, Pavla Crispi, Olivier Marie, Barbara Koedooder, Coco Obernosterer, Ingrid |
author_sort |
Hernandez-magana, Alejandra Elisa |
title |
Prokaryotic diversity and activity in contrasting productivity regimes in late summer in the Kerguelen region (Southern Ocean) |
title_short |
Prokaryotic diversity and activity in contrasting productivity regimes in late summer in the Kerguelen region (Southern Ocean) |
title_full |
Prokaryotic diversity and activity in contrasting productivity regimes in late summer in the Kerguelen region (Southern Ocean) |
title_fullStr |
Prokaryotic diversity and activity in contrasting productivity regimes in late summer in the Kerguelen region (Southern Ocean) |
title_full_unstemmed |
Prokaryotic diversity and activity in contrasting productivity regimes in late summer in the Kerguelen region (Southern Ocean) |
title_sort |
prokaryotic diversity and activity in contrasting productivity regimes in late summer in the kerguelen region (southern ocean) |
publisher |
Elsevier BV |
publishDate |
2021 |
url |
https://archimer.ifremer.fr/doc/00694/80583/83802.pdf https://doi.org/10.1016/j.jmarsys.2021.103561 https://archimer.ifremer.fr/doc/00694/80583/ |
long_lat |
ENVELOPE(69.500,69.500,-49.250,-49.250) |
geographic |
Austral Indian Kerguelen Kerguelen Island Southern Ocean |
geographic_facet |
Austral Indian Kerguelen Kerguelen Island Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
Journal Of Marine Systems (0924-7963) (Elsevier BV), 2021-09 , Vol. 221 , P. 103561 (12p.) |
op_relation |
https://archimer.ifremer.fr/doc/00694/80583/83802.pdf doi:10.1016/j.jmarsys.2021.103561 https://archimer.ifremer.fr/doc/00694/80583/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.jmarsys.2021.103561 |
container_title |
Journal of Marine Systems |
container_volume |
221 |
container_start_page |
103561 |
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1776204050445893632 |