Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic
Variation in the microbial cycling of nutrients and carbon in the ocean is an emergent property of complex planktonic communities. While recent findings have considerably expanded our understanding of the diversity and distribution of nitrogen (N2) fixing marine diazotrophs, knowledge gaps remain re...
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2021
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ftarchimer:oai:archimer.ifremer.fr:79677 2023-05-15T17:30:22+02:00 Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic Wang, Seaver Tang, Weiyi Delage, Erwan Gifford, Scott Whitby, Hannah González, Aridane G. Eveillard, Damien Planquette, Helene Cassar, Nicolas 2021-03 application/pdf https://archimer.ifremer.fr/doc/00685/79677/82454.pdf https://archimer.ifremer.fr/doc/00685/79677/82455.docx https://doi.org/10.1038/s41598-021-84969-1 https://archimer.ifremer.fr/doc/00685/79677/ eng eng Springer Science and Business Media LLC https://archimer.ifremer.fr/doc/00685/79677/82454.pdf https://archimer.ifremer.fr/doc/00685/79677/82455.docx doi:10.1038/s41598-021-84969-1 https://archimer.ifremer.fr/doc/00685/79677/ info:eu-repo/semantics/openAccess restricted use Scientific Reports (2045-2322) (Springer Science and Business Media LLC), 2021-03 , Vol. 11 , N. 1 , P. 5508 (14p.) text Publication info:eu-repo/semantics/article 2021 ftarchimer https://doi.org/10.1038/s41598-021-84969-1 2021-09-23T20:37:08Z Variation in the microbial cycling of nutrients and carbon in the ocean is an emergent property of complex planktonic communities. While recent findings have considerably expanded our understanding of the diversity and distribution of nitrogen (N2) fixing marine diazotrophs, knowledge gaps remain regarding ecological interactions between diazotrophs and other community members. Using quantitative 16S and 18S V4 rDNA amplicon sequencing, we surveyed eukaryotic and prokaryotic microbial communities from samples collected in August 2016 and 2017 across the Western North Atlantic. Leveraging and significantly expanding an earlier published 2015 molecular dataset, we examined microbial community structure and ecological co-occurrence relationships associated with intense hotspots of N2 fixation previously reported at sites off the Southern New England Shelf and Mid-Atlantic Bight. Overall, we observed a negative relationship between eukaryotic diversity and both N2 fixation and net community production (NCP). Maximum N2 fixation rates occurred at sites with high abundances of mixotrophic stramenopiles, notably Chrysophyceae. Network analysis revealed such stramenopiles to be keystone taxa alongside the haptophyte diazotroph host Braarudosphaera bigelowii and chlorophytes. Our findings highlight an intriguing relationship between marine stramenopiles and high N2 fixation coastal sites. Article in Journal/Newspaper North Atlantic Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Scientific Reports 11 1 |
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Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
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ftarchimer |
language |
English |
description |
Variation in the microbial cycling of nutrients and carbon in the ocean is an emergent property of complex planktonic communities. While recent findings have considerably expanded our understanding of the diversity and distribution of nitrogen (N2) fixing marine diazotrophs, knowledge gaps remain regarding ecological interactions between diazotrophs and other community members. Using quantitative 16S and 18S V4 rDNA amplicon sequencing, we surveyed eukaryotic and prokaryotic microbial communities from samples collected in August 2016 and 2017 across the Western North Atlantic. Leveraging and significantly expanding an earlier published 2015 molecular dataset, we examined microbial community structure and ecological co-occurrence relationships associated with intense hotspots of N2 fixation previously reported at sites off the Southern New England Shelf and Mid-Atlantic Bight. Overall, we observed a negative relationship between eukaryotic diversity and both N2 fixation and net community production (NCP). Maximum N2 fixation rates occurred at sites with high abundances of mixotrophic stramenopiles, notably Chrysophyceae. Network analysis revealed such stramenopiles to be keystone taxa alongside the haptophyte diazotroph host Braarudosphaera bigelowii and chlorophytes. Our findings highlight an intriguing relationship between marine stramenopiles and high N2 fixation coastal sites. |
format |
Article in Journal/Newspaper |
author |
Wang, Seaver Tang, Weiyi Delage, Erwan Gifford, Scott Whitby, Hannah González, Aridane G. Eveillard, Damien Planquette, Helene Cassar, Nicolas |
spellingShingle |
Wang, Seaver Tang, Weiyi Delage, Erwan Gifford, Scott Whitby, Hannah González, Aridane G. Eveillard, Damien Planquette, Helene Cassar, Nicolas Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
author_facet |
Wang, Seaver Tang, Weiyi Delage, Erwan Gifford, Scott Whitby, Hannah González, Aridane G. Eveillard, Damien Planquette, Helene Cassar, Nicolas |
author_sort |
Wang, Seaver |
title |
Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
title_short |
Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
title_full |
Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
title_fullStr |
Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
title_full_unstemmed |
Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
title_sort |
investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western north atlantic |
publisher |
Springer Science and Business Media LLC |
publishDate |
2021 |
url |
https://archimer.ifremer.fr/doc/00685/79677/82454.pdf https://archimer.ifremer.fr/doc/00685/79677/82455.docx https://doi.org/10.1038/s41598-021-84969-1 https://archimer.ifremer.fr/doc/00685/79677/ |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Scientific Reports (2045-2322) (Springer Science and Business Media LLC), 2021-03 , Vol. 11 , N. 1 , P. 5508 (14p.) |
op_relation |
https://archimer.ifremer.fr/doc/00685/79677/82454.pdf https://archimer.ifremer.fr/doc/00685/79677/82455.docx doi:10.1038/s41598-021-84969-1 https://archimer.ifremer.fr/doc/00685/79677/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1038/s41598-021-84969-1 |
container_title |
Scientific Reports |
container_volume |
11 |
container_issue |
1 |
_version_ |
1766126723176333312 |