Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas )
Oysters are keystone species that use external fertilization as a sexual mode. The gametes are planktonic and face a wide range of stressors, including plastic litter. Nanoplastics are of increasing concern because their size allows pronounced interactions with biological membranes, making them a po...
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Online Access: | https://archimer.ifremer.fr/doc/00643/75522/78210.pdf https://doi.org/10.1080/17435390.2020.1808104 https://archimer.ifremer.fr/doc/00643/75522/ |
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ftarchimer:oai:archimer.ifremer.fr:75522 2023-05-15T15:58:57+02:00 Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas ) Tallec, Kevin Paul-pont, Ika Boulais, Myrina Le Goïc, Nelly Gonzalez Fernandez, C. Le Grand, Fabienne Bideau, Antoine Quéré, Claudie Cassone, A.-l. Lambert, Christophe Soudant, Philippe Huvet, Arnaud 2020-09 application/pdf https://archimer.ifremer.fr/doc/00643/75522/78210.pdf https://doi.org/10.1080/17435390.2020.1808104 https://archimer.ifremer.fr/doc/00643/75522/ eng eng Informa UK Limited https://archimer.ifremer.fr/doc/00643/75522/78210.pdf doi:10.1080/17435390.2020.1808104 https://archimer.ifremer.fr/doc/00643/75522/ info:eu-repo/semantics/openAccess restricted use Nanotoxicology (1743-5390) (Informa UK Limited), 2020-09 , Vol. 14 , N. 8 , P. 1039-1057 Nanoplastics oyster spermatozoa motility reproductive success text Publication info:eu-repo/semantics/article 2020 ftarchimer https://doi.org/10.1080/17435390.2020.1808104 2021-09-23T20:35:46Z Oysters are keystone species that use external fertilization as a sexual mode. The gametes are planktonic and face a wide range of stressors, including plastic litter. Nanoplastics are of increasing concern because their size allows pronounced interactions with biological membranes, making them a potential hazard to marine life. In the present study, oyster spermatozoa were exposed for 1 h to various doses (from 0.1 to 25 µg mL−1) of 50-nm polystyrene beads with amine (50-NH2 beads) or carboxyl (50-COOH beads) functions. Microscopy revealed adhesion of particles to the spermatozoa membranes, but no translocation of either particle type into cells. Nevertheless, the 50-NH2 beads at 10 µg mL−1 induced a high spermiotoxicity, characterized by a decrease in the percentage of motile spermatozoa (−79%) and in the velocity (−62%) compared to control spermatozoa, with an overall drop in embryogenesis success (−59%). This major reproduction failure could be linked to a homeostasis disruption in exposed spermatozoa. The 50-COOH beads hampered spermatozoa motility only when administered at 25 µg mL−1 and caused a decrease in the percentage of motile spermatozoa (−66%) and in the velocity (−38%), but did not affect embryogenesis success. Microscopy analyses indicated these effects were probably due to physical blockages by microscale aggregates formed by the 50-COOH beads in seawater. This toxicological study emphasizes that oyster spermatozoa are a useful and sensitive model for (i) deciphering the fine interactions underpinning nanoplastic toxicity and (ii) evaluating adverse effects of plastic nanoparticles on marine biota while waiting for their concentration to be known in the environment. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Nanotoxicology 14 8 1039 1057 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Nanoplastics oyster spermatozoa motility reproductive success |
spellingShingle |
Nanoplastics oyster spermatozoa motility reproductive success Tallec, Kevin Paul-pont, Ika Boulais, Myrina Le Goïc, Nelly Gonzalez Fernandez, C. Le Grand, Fabienne Bideau, Antoine Quéré, Claudie Cassone, A.-l. Lambert, Christophe Soudant, Philippe Huvet, Arnaud Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas ) |
topic_facet |
Nanoplastics oyster spermatozoa motility reproductive success |
description |
Oysters are keystone species that use external fertilization as a sexual mode. The gametes are planktonic and face a wide range of stressors, including plastic litter. Nanoplastics are of increasing concern because their size allows pronounced interactions with biological membranes, making them a potential hazard to marine life. In the present study, oyster spermatozoa were exposed for 1 h to various doses (from 0.1 to 25 µg mL−1) of 50-nm polystyrene beads with amine (50-NH2 beads) or carboxyl (50-COOH beads) functions. Microscopy revealed adhesion of particles to the spermatozoa membranes, but no translocation of either particle type into cells. Nevertheless, the 50-NH2 beads at 10 µg mL−1 induced a high spermiotoxicity, characterized by a decrease in the percentage of motile spermatozoa (−79%) and in the velocity (−62%) compared to control spermatozoa, with an overall drop in embryogenesis success (−59%). This major reproduction failure could be linked to a homeostasis disruption in exposed spermatozoa. The 50-COOH beads hampered spermatozoa motility only when administered at 25 µg mL−1 and caused a decrease in the percentage of motile spermatozoa (−66%) and in the velocity (−38%), but did not affect embryogenesis success. Microscopy analyses indicated these effects were probably due to physical blockages by microscale aggregates formed by the 50-COOH beads in seawater. This toxicological study emphasizes that oyster spermatozoa are a useful and sensitive model for (i) deciphering the fine interactions underpinning nanoplastic toxicity and (ii) evaluating adverse effects of plastic nanoparticles on marine biota while waiting for their concentration to be known in the environment. |
format |
Article in Journal/Newspaper |
author |
Tallec, Kevin Paul-pont, Ika Boulais, Myrina Le Goïc, Nelly Gonzalez Fernandez, C. Le Grand, Fabienne Bideau, Antoine Quéré, Claudie Cassone, A.-l. Lambert, Christophe Soudant, Philippe Huvet, Arnaud |
author_facet |
Tallec, Kevin Paul-pont, Ika Boulais, Myrina Le Goïc, Nelly Gonzalez Fernandez, C. Le Grand, Fabienne Bideau, Antoine Quéré, Claudie Cassone, A.-l. Lambert, Christophe Soudant, Philippe Huvet, Arnaud |
author_sort |
Tallec, Kevin |
title |
Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas ) |
title_short |
Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas ) |
title_full |
Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas ) |
title_fullStr |
Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas ) |
title_full_unstemmed |
Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas ) |
title_sort |
nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( crassostrea gigas ) |
publisher |
Informa UK Limited |
publishDate |
2020 |
url |
https://archimer.ifremer.fr/doc/00643/75522/78210.pdf https://doi.org/10.1080/17435390.2020.1808104 https://archimer.ifremer.fr/doc/00643/75522/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Nanotoxicology (1743-5390) (Informa UK Limited), 2020-09 , Vol. 14 , N. 8 , P. 1039-1057 |
op_relation |
https://archimer.ifremer.fr/doc/00643/75522/78210.pdf doi:10.1080/17435390.2020.1808104 https://archimer.ifremer.fr/doc/00643/75522/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1080/17435390.2020.1808104 |
container_title |
Nanotoxicology |
container_volume |
14 |
container_issue |
8 |
container_start_page |
1039 |
op_container_end_page |
1057 |
_version_ |
1766394727425376256 |