Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas
Harmful algal blooms (HABs) of toxic species of the dinoflagellate genus Dinophysis are a threat to human health as they are mainly responsible for diarrheic shellfish poisoning (DSP) in the consumers of contaminated shellfish. Such contamination leads to shellfish farm closures causing major econom...
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2020
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Online Access: | https://archimer.ifremer.fr/doc/00630/74167/73767.pdf https://doi.org/10.1016/j.envpol.2020.114840 https://archimer.ifremer.fr/doc/00630/74167/ |
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ftarchimer:oai:archimer.ifremer.fr:74167 2023-05-15T15:58:15+02:00 Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas Gaillard, Sylvain Le Goïc, Nelly Malo, Florent Boulais, Myrina Fabioux, Caroline Zaccagnini, Lucas Carpentier, Liliane Sibat, Manoella Réveillon, Damien Séchet, Veronique Hess, Philipp Hégaret, Helene 2020-10 application/pdf https://archimer.ifremer.fr/doc/00630/74167/73767.pdf https://doi.org/10.1016/j.envpol.2020.114840 https://archimer.ifremer.fr/doc/00630/74167/ eng eng Elsevier BV https://archimer.ifremer.fr/doc/00630/74167/73767.pdf doi:10.1016/j.envpol.2020.114840 https://archimer.ifremer.fr/doc/00630/74167/ info:eu-repo/semantics/openAccess restricted use Environmental Pollution (0269-7491) (Elsevier BV), 2020-10 , Vol. 265 , N. Part B , P. 114840 (10p.) Dinophysis spp Okadaic acid Pectenotoxins Oyster gametes Fertilization success text Publication info:eu-repo/semantics/article 2020 ftarchimer https://doi.org/10.1016/j.envpol.2020.114840 2021-09-23T20:35:08Z Harmful algal blooms (HABs) of toxic species of the dinoflagellate genus Dinophysis are a threat to human health as they are mainly responsible for diarrheic shellfish poisoning (DSP) in the consumers of contaminated shellfish. Such contamination leads to shellfish farm closures causing major economic and social issues. The direct effects of numerous HAB species have been demonstrated on adult bivalves, whereas the effects on critical early life stages remain relatively unexplored. The present study aimed to determine the in vitro effects of either cultivated strains of D. sacculus and D. acuminata isolated from France or their associated toxins (i.e. okadaic acid (OA) and pectenotoxin 2 (PTX2)) on the quality of the gametes of the Pacific oyster Crassostrea gigas. This was performed by assessing the ROS production and viability of the gametes using flow cytometry, and fertilization success using microscopic counts. Oocytes were more affected than spermatozoa and their mortality and ROS production increased in the presence of D. sacculus and PTX2, respectively. A decrease in fertilization success was observed at concentrations as low as 0.5 cell mL−1 of Dinophysis spp. and 5 nM of PTX2, whereas no effect of OA could be observed. The effect on fertilization success was higher when both gamete types were concomitantly exposed compared to separate exposures, suggesting a synergistic effect. Our results also suggest that the effects could be due to cell-to-cell contact. These results highlight a potential effect of Dinophysis spp. and PTX2 on reproduction and recruitment of the Pacific oyster. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Environmental Pollution 265 114840 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Dinophysis spp Okadaic acid Pectenotoxins Oyster gametes Fertilization success |
spellingShingle |
Dinophysis spp Okadaic acid Pectenotoxins Oyster gametes Fertilization success Gaillard, Sylvain Le Goïc, Nelly Malo, Florent Boulais, Myrina Fabioux, Caroline Zaccagnini, Lucas Carpentier, Liliane Sibat, Manoella Réveillon, Damien Séchet, Veronique Hess, Philipp Hégaret, Helene Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas |
topic_facet |
Dinophysis spp Okadaic acid Pectenotoxins Oyster gametes Fertilization success |
description |
Harmful algal blooms (HABs) of toxic species of the dinoflagellate genus Dinophysis are a threat to human health as they are mainly responsible for diarrheic shellfish poisoning (DSP) in the consumers of contaminated shellfish. Such contamination leads to shellfish farm closures causing major economic and social issues. The direct effects of numerous HAB species have been demonstrated on adult bivalves, whereas the effects on critical early life stages remain relatively unexplored. The present study aimed to determine the in vitro effects of either cultivated strains of D. sacculus and D. acuminata isolated from France or their associated toxins (i.e. okadaic acid (OA) and pectenotoxin 2 (PTX2)) on the quality of the gametes of the Pacific oyster Crassostrea gigas. This was performed by assessing the ROS production and viability of the gametes using flow cytometry, and fertilization success using microscopic counts. Oocytes were more affected than spermatozoa and their mortality and ROS production increased in the presence of D. sacculus and PTX2, respectively. A decrease in fertilization success was observed at concentrations as low as 0.5 cell mL−1 of Dinophysis spp. and 5 nM of PTX2, whereas no effect of OA could be observed. The effect on fertilization success was higher when both gamete types were concomitantly exposed compared to separate exposures, suggesting a synergistic effect. Our results also suggest that the effects could be due to cell-to-cell contact. These results highlight a potential effect of Dinophysis spp. and PTX2 on reproduction and recruitment of the Pacific oyster. |
format |
Article in Journal/Newspaper |
author |
Gaillard, Sylvain Le Goïc, Nelly Malo, Florent Boulais, Myrina Fabioux, Caroline Zaccagnini, Lucas Carpentier, Liliane Sibat, Manoella Réveillon, Damien Séchet, Veronique Hess, Philipp Hégaret, Helene |
author_facet |
Gaillard, Sylvain Le Goïc, Nelly Malo, Florent Boulais, Myrina Fabioux, Caroline Zaccagnini, Lucas Carpentier, Liliane Sibat, Manoella Réveillon, Damien Séchet, Veronique Hess, Philipp Hégaret, Helene |
author_sort |
Gaillard, Sylvain |
title |
Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas |
title_short |
Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas |
title_full |
Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas |
title_fullStr |
Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas |
title_sort |
cultures of dinophysis sacculus, d. acuminata and pectenotoxin 2 affect gametes and fertilization success of the pacific oyster, crassostrea gigas |
publisher |
Elsevier BV |
publishDate |
2020 |
url |
https://archimer.ifremer.fr/doc/00630/74167/73767.pdf https://doi.org/10.1016/j.envpol.2020.114840 https://archimer.ifremer.fr/doc/00630/74167/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Environmental Pollution (0269-7491) (Elsevier BV), 2020-10 , Vol. 265 , N. Part B , P. 114840 (10p.) |
op_relation |
https://archimer.ifremer.fr/doc/00630/74167/73767.pdf doi:10.1016/j.envpol.2020.114840 https://archimer.ifremer.fr/doc/00630/74167/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.envpol.2020.114840 |
container_title |
Environmental Pollution |
container_volume |
265 |
container_start_page |
114840 |
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1766393983025545216 |