Oyster hemolymph is a complex and dynamic ecosystem hosting bacteria, protists and viruses
Background The impact of the microbiota on host fitness has so far mainly been demonstrated for the bacterial microbiome. We know much less about host-associated protist and viral communities, largely due to technical issues. However, all microorganisms within a microbiome potentially interact with...
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ftarchimer:oai:archimer.ifremer.fr:73916 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Oyster genetic background Hemolymph microbiota dynamics Early-life microbiota Trans-kingdom interactions Crassostrea gigas Within-host ecosystem |
spellingShingle |
Oyster genetic background Hemolymph microbiota dynamics Early-life microbiota Trans-kingdom interactions Crassostrea gigas Within-host ecosystem Dupont, Samuel Lokmer, A. Corre, E. Auguet, Jean-christophe Petton, Bruno Toulza, E. Montagnani, Caroline Tanguy, G. Pecqueur, D. Salmeron, C. Guillou, L. Desnues, C. La Scola, B. Bou Khalil, J. De Lorgeril, Julien Mitta, Guillaume Gueguen, Yannick Escoubas, Jean Michel Oyster hemolymph is a complex and dynamic ecosystem hosting bacteria, protists and viruses |
topic_facet |
Oyster genetic background Hemolymph microbiota dynamics Early-life microbiota Trans-kingdom interactions Crassostrea gigas Within-host ecosystem |
description |
Background The impact of the microbiota on host fitness has so far mainly been demonstrated for the bacterial microbiome. We know much less about host-associated protist and viral communities, largely due to technical issues. However, all microorganisms within a microbiome potentially interact with each other as well as with the host and the environment, therefore likely affecting the host health. Results We set out to explore how environmental and host factors shape the composition and diversity of bacterial, protist and viral microbial communities in the Pacific oyster hemolymph, both in health and disease. To do so, five oyster families differing in susceptibility to the Pacific oyster mortality syndrome were reared in hatchery and transplanted into a natural environment either before or during a disease outbreak. Using metabarcoding and shotgun metagenomics, we demonstrate that hemolymph can be considered as an ecological niche hosting bacterial, protist and viral communities, each of them shaped by different factors and distinct from the corresponding communities in the surrounding seawater. Overall, we found that hemolymph microbiota is more strongly shaped by the environment than by host genetic background. Co-occurrence network analyses suggest a disruption of the microbial network after transplantation into natural environment during both non-infectious and infectious periods. Whereas we could not identify a common microbial community signature for healthy animals, OsHV-1 μVar virus dominated the hemolymph virome during the disease outbreak, without significant modifications of other microbiota components. Conclusion Our study shows that oyster hemolymph is a complex ecosystem containing diverse bacteria, protists and viruses, whose composition and dynamics are primarily determined by the environment. However, all of these are also shaped by oyster genetic backgrounds, indicating they indeed interact with the oyster host and are therefore not only of transient character. Although it seems that the three ... |
format |
Article in Journal/Newspaper |
author |
Dupont, Samuel Lokmer, A. Corre, E. Auguet, Jean-christophe Petton, Bruno Toulza, E. Montagnani, Caroline Tanguy, G. Pecqueur, D. Salmeron, C. Guillou, L. Desnues, C. La Scola, B. Bou Khalil, J. De Lorgeril, Julien Mitta, Guillaume Gueguen, Yannick Escoubas, Jean Michel |
author_facet |
Dupont, Samuel Lokmer, A. Corre, E. Auguet, Jean-christophe Petton, Bruno Toulza, E. Montagnani, Caroline Tanguy, G. Pecqueur, D. Salmeron, C. Guillou, L. Desnues, C. La Scola, B. Bou Khalil, J. De Lorgeril, Julien Mitta, Guillaume Gueguen, Yannick Escoubas, Jean Michel |
author_sort |
Dupont, Samuel |
title |
Oyster hemolymph is a complex and dynamic ecosystem hosting bacteria, protists and viruses |
title_short |
Oyster hemolymph is a complex and dynamic ecosystem hosting bacteria, protists and viruses |
title_full |
Oyster hemolymph is a complex and dynamic ecosystem hosting bacteria, protists and viruses |
title_fullStr |
Oyster hemolymph is a complex and dynamic ecosystem hosting bacteria, protists and viruses |
title_full_unstemmed |
Oyster hemolymph is a complex and dynamic ecosystem hosting bacteria, protists and viruses |
title_sort |
oyster hemolymph is a complex and dynamic ecosystem hosting bacteria, protists and viruses |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
https://archimer.ifremer.fr/doc/00627/73916/73254.pdf https://archimer.ifremer.fr/doc/00627/73916/73258.xlsx https://archimer.ifremer.fr/doc/00627/73916/73259.xlsx https://archimer.ifremer.fr/doc/00627/73916/73260.xlsx https://archimer.ifremer.fr/doc/00627/73916/73261.xlsx https://archimer.ifremer.fr/doc/00627/73916/73262.pptx https://archimer.ifremer.fr/doc/00627/73916/73263.xlsx https://archimer.ifremer.fr/doc/00627/73916/73264.xlsx https://archimer.ifremer.fr/doc/00627/73916/73265.xlsx https://archimer.ifremer.fr/doc/00627/73916/73266.xlsx https://archimer.ifremer.fr/doc/00627/73916/73267.xlsx https://archimer.ifremer.fr/doc/00627/73916/73268.xlsx https://archimer.ifremer.fr/doc/00627/73916/73269.xlsx https://archimer.ifremer.fr/doc/00627/73916/73270.xlsx https://doi.org/10.1186/s42523-020-00032-w https://archimer.ifremer.fr/doc/00627/73916/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Animal Microbiome (2524-4671) (Springer Science and Business Media LLC), 2020-04 , Vol. 2 , N. 1 , P. 12 (16p.) |
op_relation |
info:eu-repo/grantAgreement/EC/FP7/678589/EU//VIVALDI https://archimer.ifremer.fr/doc/00627/73916/73254.pdf https://archimer.ifremer.fr/doc/00627/73916/73258.xlsx https://archimer.ifremer.fr/doc/00627/73916/73259.xlsx https://archimer.ifremer.fr/doc/00627/73916/73260.xlsx https://archimer.ifremer.fr/doc/00627/73916/73261.xlsx https://archimer.ifremer.fr/doc/00627/73916/73262.pptx https://archimer.ifremer.fr/doc/00627/73916/73263.xlsx https://archimer.ifremer.fr/doc/00627/73916/73264.xlsx https://archimer.ifremer.fr/doc/00627/73916/73265.xlsx https://archimer.ifremer.fr/doc/00627/73916/73266.xlsx https://archimer.ifremer.fr/doc/00627/73916/73267.xlsx https://archimer.ifremer.fr/doc/00627/73916/73268.xlsx https://archimer.ifremer.fr/doc/00627/73916/73269.xlsx https://archimer.ifremer.fr/doc/00627/73916/73270.xlsx doi:10.1186/s42523-020-00032-w https://archimer.ifremer.fr/doc/00627/73916/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1186/s42523-020-00032-w |
container_title |
Animal Microbiome |
container_volume |
2 |
container_issue |
1 |
_version_ |
1782333433917210624 |
spelling |
ftarchimer:oai:archimer.ifremer.fr:73916 2023-11-12T04:16:18+01:00 Oyster hemolymph is a complex and dynamic ecosystem hosting bacteria, protists and viruses Dupont, Samuel Lokmer, A. Corre, E. Auguet, Jean-christophe Petton, Bruno Toulza, E. Montagnani, Caroline Tanguy, G. Pecqueur, D. Salmeron, C. Guillou, L. Desnues, C. La Scola, B. Bou Khalil, J. De Lorgeril, Julien Mitta, Guillaume Gueguen, Yannick Escoubas, Jean Michel 2020-04 application/pdf https://archimer.ifremer.fr/doc/00627/73916/73254.pdf https://archimer.ifremer.fr/doc/00627/73916/73258.xlsx https://archimer.ifremer.fr/doc/00627/73916/73259.xlsx https://archimer.ifremer.fr/doc/00627/73916/73260.xlsx https://archimer.ifremer.fr/doc/00627/73916/73261.xlsx https://archimer.ifremer.fr/doc/00627/73916/73262.pptx https://archimer.ifremer.fr/doc/00627/73916/73263.xlsx https://archimer.ifremer.fr/doc/00627/73916/73264.xlsx https://archimer.ifremer.fr/doc/00627/73916/73265.xlsx https://archimer.ifremer.fr/doc/00627/73916/73266.xlsx https://archimer.ifremer.fr/doc/00627/73916/73267.xlsx https://archimer.ifremer.fr/doc/00627/73916/73268.xlsx https://archimer.ifremer.fr/doc/00627/73916/73269.xlsx https://archimer.ifremer.fr/doc/00627/73916/73270.xlsx https://doi.org/10.1186/s42523-020-00032-w https://archimer.ifremer.fr/doc/00627/73916/ eng eng Springer Science and Business Media LLC info:eu-repo/grantAgreement/EC/FP7/678589/EU//VIVALDI https://archimer.ifremer.fr/doc/00627/73916/73254.pdf https://archimer.ifremer.fr/doc/00627/73916/73258.xlsx https://archimer.ifremer.fr/doc/00627/73916/73259.xlsx https://archimer.ifremer.fr/doc/00627/73916/73260.xlsx https://archimer.ifremer.fr/doc/00627/73916/73261.xlsx https://archimer.ifremer.fr/doc/00627/73916/73262.pptx https://archimer.ifremer.fr/doc/00627/73916/73263.xlsx https://archimer.ifremer.fr/doc/00627/73916/73264.xlsx https://archimer.ifremer.fr/doc/00627/73916/73265.xlsx https://archimer.ifremer.fr/doc/00627/73916/73266.xlsx https://archimer.ifremer.fr/doc/00627/73916/73267.xlsx https://archimer.ifremer.fr/doc/00627/73916/73268.xlsx https://archimer.ifremer.fr/doc/00627/73916/73269.xlsx https://archimer.ifremer.fr/doc/00627/73916/73270.xlsx doi:10.1186/s42523-020-00032-w https://archimer.ifremer.fr/doc/00627/73916/ info:eu-repo/semantics/openAccess restricted use Animal Microbiome (2524-4671) (Springer Science and Business Media LLC), 2020-04 , Vol. 2 , N. 1 , P. 12 (16p.) Oyster genetic background Hemolymph microbiota dynamics Early-life microbiota Trans-kingdom interactions Crassostrea gigas Within-host ecosystem text Article info:eu-repo/semantics/article 2020 ftarchimer https://doi.org/10.1186/s42523-020-00032-w 2023-10-17T22:51:11Z Background The impact of the microbiota on host fitness has so far mainly been demonstrated for the bacterial microbiome. We know much less about host-associated protist and viral communities, largely due to technical issues. However, all microorganisms within a microbiome potentially interact with each other as well as with the host and the environment, therefore likely affecting the host health. Results We set out to explore how environmental and host factors shape the composition and diversity of bacterial, protist and viral microbial communities in the Pacific oyster hemolymph, both in health and disease. To do so, five oyster families differing in susceptibility to the Pacific oyster mortality syndrome were reared in hatchery and transplanted into a natural environment either before or during a disease outbreak. Using metabarcoding and shotgun metagenomics, we demonstrate that hemolymph can be considered as an ecological niche hosting bacterial, protist and viral communities, each of them shaped by different factors and distinct from the corresponding communities in the surrounding seawater. Overall, we found that hemolymph microbiota is more strongly shaped by the environment than by host genetic background. Co-occurrence network analyses suggest a disruption of the microbial network after transplantation into natural environment during both non-infectious and infectious periods. Whereas we could not identify a common microbial community signature for healthy animals, OsHV-1 μVar virus dominated the hemolymph virome during the disease outbreak, without significant modifications of other microbiota components. Conclusion Our study shows that oyster hemolymph is a complex ecosystem containing diverse bacteria, protists and viruses, whose composition and dynamics are primarily determined by the environment. However, all of these are also shaped by oyster genetic backgrounds, indicating they indeed interact with the oyster host and are therefore not only of transient character. Although it seems that the three ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Animal Microbiome 2 1 |