Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts
Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Made...
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MDPI AG
2020
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Online Access: | https://archimer.ifremer.fr/doc/00624/73630/73067.pdf https://archimer.ifremer.fr/doc/00624/73630/73068.pdf https://doi.org/10.3390/toxins12040267 https://archimer.ifremer.fr/doc/00624/73630/ |
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ftarchimer:oai:archimer.ifremer.fr:73630 2023-05-15T17:41:30+02:00 Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts Estevez, Pablo Sibat, Manoella Leão-martins, José Manuel Reis Costa, Pedro Gago-martínez, Ana Hess, Philipp 2020-04 application/pdf https://archimer.ifremer.fr/doc/00624/73630/73067.pdf https://archimer.ifremer.fr/doc/00624/73630/73068.pdf https://doi.org/10.3390/toxins12040267 https://archimer.ifremer.fr/doc/00624/73630/ eng eng MDPI AG https://archimer.ifremer.fr/doc/00624/73630/73067.pdf https://archimer.ifremer.fr/doc/00624/73630/73068.pdf doi:10.3390/toxins12040267 https://archimer.ifremer.fr/doc/00624/73630/ info:eu-repo/semantics/openAccess restricted use Toxins (2072-6651) (MDPI AG), 2020-04 , Vol. 12 , N. 4 , P. 267 (7p.) ciguatoxins HRMS Q-TOF ciguatera poisoning C-CTX1 fragmentation pathways text Publication info:eu-repo/semantics/article 2020 ftarchimer https://doi.org/10.3390/toxins12040267 2021-09-23T20:35:00Z Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed the initial confirmation of the presence of C-CTX1 in contaminated fish samples from the abovementioned areas, nevertheless the lack of commercially available reference materials for these particular ciguatoxin (CTX) analogues has been a major limitation to progress research. The EuroCigua project allowed the preparation of C-CTX1 laboratory reference material (LRM) from fish species (Seriola fasciata) from the Madeira archipelago (Portugal). This reference material was used to implement a liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) for the detection of C-CTX1, acquisition of full-scan as well as collision-induced mass spectra of this particular analogue. Fragmentation pathways were proposed based on fragments obtained. The optimized LC-HRMS method was then applied to confirm C-CTX1 in fish (Bodianus scrofa) caught in the Selvagens Islands (Portugal). Article in Journal/Newspaper Northeast Atlantic Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Toxins 12 4 267 |
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Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
ciguatoxins HRMS Q-TOF ciguatera poisoning C-CTX1 fragmentation pathways |
spellingShingle |
ciguatoxins HRMS Q-TOF ciguatera poisoning C-CTX1 fragmentation pathways Estevez, Pablo Sibat, Manoella Leão-martins, José Manuel Reis Costa, Pedro Gago-martínez, Ana Hess, Philipp Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
topic_facet |
ciguatoxins HRMS Q-TOF ciguatera poisoning C-CTX1 fragmentation pathways |
description |
Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed the initial confirmation of the presence of C-CTX1 in contaminated fish samples from the abovementioned areas, nevertheless the lack of commercially available reference materials for these particular ciguatoxin (CTX) analogues has been a major limitation to progress research. The EuroCigua project allowed the preparation of C-CTX1 laboratory reference material (LRM) from fish species (Seriola fasciata) from the Madeira archipelago (Portugal). This reference material was used to implement a liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) for the detection of C-CTX1, acquisition of full-scan as well as collision-induced mass spectra of this particular analogue. Fragmentation pathways were proposed based on fragments obtained. The optimized LC-HRMS method was then applied to confirm C-CTX1 in fish (Bodianus scrofa) caught in the Selvagens Islands (Portugal). |
format |
Article in Journal/Newspaper |
author |
Estevez, Pablo Sibat, Manoella Leão-martins, José Manuel Reis Costa, Pedro Gago-martínez, Ana Hess, Philipp |
author_facet |
Estevez, Pablo Sibat, Manoella Leão-martins, José Manuel Reis Costa, Pedro Gago-martínez, Ana Hess, Philipp |
author_sort |
Estevez, Pablo |
title |
Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
title_short |
Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
title_full |
Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
title_fullStr |
Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
title_full_unstemmed |
Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
title_sort |
liquid chromatography coupled to high-resolution mass spectrometry for the confirmation of caribbean ciguatoxin-1 as the main toxin responsible for ciguatera poisoning caused by fish from european atlantic coasts |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://archimer.ifremer.fr/doc/00624/73630/73067.pdf https://archimer.ifremer.fr/doc/00624/73630/73068.pdf https://doi.org/10.3390/toxins12040267 https://archimer.ifremer.fr/doc/00624/73630/ |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_source |
Toxins (2072-6651) (MDPI AG), 2020-04 , Vol. 12 , N. 4 , P. 267 (7p.) |
op_relation |
https://archimer.ifremer.fr/doc/00624/73630/73067.pdf https://archimer.ifremer.fr/doc/00624/73630/73068.pdf doi:10.3390/toxins12040267 https://archimer.ifremer.fr/doc/00624/73630/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.3390/toxins12040267 |
container_title |
Toxins |
container_volume |
12 |
container_issue |
4 |
container_start_page |
267 |
_version_ |
1766143104845348864 |