Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas
The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism...
Published in: | Autophagy |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Informa UK Limited
2020
|
Subjects: | |
Online Access: | https://archimer.ifremer.fr/doc/00606/71785/70270.pdf https://archimer.ifremer.fr/doc/00606/71785/70271.pdf https://archimer.ifremer.fr/doc/00606/71785/70272.pdf https://archimer.ifremer.fr/doc/00606/71785/70273.pdf https://archimer.ifremer.fr/doc/00606/71785/70274.pdf https://doi.org/10.1080/15548627.2020.1713643 https://archimer.ifremer.fr/doc/00606/71785/ |
id |
ftarchimer:oai:archimer.ifremer.fr:71785 |
---|---|
record_format |
openpolar |
spelling |
ftarchimer:oai:archimer.ifremer.fr:71785 2023-05-15T15:58:03+02:00 Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas Picot, Sandy Faury, Nicole Arzul, Isabelle Chollet, Bruno Renault, Tristan Morga, Benjamin 2020-11 application/pdf https://archimer.ifremer.fr/doc/00606/71785/70270.pdf https://archimer.ifremer.fr/doc/00606/71785/70271.pdf https://archimer.ifremer.fr/doc/00606/71785/70272.pdf https://archimer.ifremer.fr/doc/00606/71785/70273.pdf https://archimer.ifremer.fr/doc/00606/71785/70274.pdf https://doi.org/10.1080/15548627.2020.1713643 https://archimer.ifremer.fr/doc/00606/71785/ eng eng Informa UK Limited https://archimer.ifremer.fr/doc/00606/71785/70270.pdf https://archimer.ifremer.fr/doc/00606/71785/70271.pdf https://archimer.ifremer.fr/doc/00606/71785/70272.pdf https://archimer.ifremer.fr/doc/00606/71785/70273.pdf https://archimer.ifremer.fr/doc/00606/71785/70274.pdf doi:10.1080/15548627.2020.1713643 https://archimer.ifremer.fr/doc/00606/71785/ info:eu-repo/semantics/openAccess restricted use Autophagy (1554-8627) (Informa UK Limited), 2020-11 , Vol. 16 , N. 11 , P. 2017-2035 Autophagy autophagy related crassostrea gigas data mining immunohistochemistry real-time PCR western blot text Publication info:eu-repo/semantics/article 2020 ftarchimer https://doi.org/10.1080/15548627.2020.1713643 2021-09-23T20:34:22Z The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism elimination. Autophagy also exists in C. gigas, and plays a role in the immune response against infections. Although this process is well-documented and conserved in most animals, it is still poorly understood in mollusks. To date, no study has provided a complete overview of the molecular mechanism of autophagy in mollusk bivalves. In this study, human and yeast ATG protein sequences and public databases (Uniprot and NCBI) were used to identify protein members of the C. gigas autophagy pathway. A total of 35 autophagy related proteins were found in the Pacific oyster. RACE-PCR was performed on several genes. Using molecular (real-time PCR) and protein-based (western blot and immunohistochemistry) approaches, the expression and localization of ATG12, ATG9, BECN1, MAP1LC3, MTOR, and SQSTM1, was investigated in different tissues of the Pacific oyster. Comparison with human and yeast counterparts demonstrated a high homology with the human autophagy pathway. The results also demonstrated that the key autophagy genes and their protein products were expressed in all the analyzed tissues of C. gigas. This study allows the characterization of the complete C. gigas autophagy pathway for the first time. Abbreviations: ATG: autophagy related; Atg1/ULK: unc-51 like autophagy activating kinase; ATG7: autophagy related 7; ATG9: autophagy related 9; ATG12: autophagy related 12; BECN1: beclin 1; BSA: bovine serum albumin; cDNA: complementary deoxyribonucleic acid; DNA: deoxyribonucleic acid; GABARAP: GABA type A receptor-associated protein; IHC: immunohistochemistry; MAP1LC3/LC3/Atg8: microtubule associated protein 1 light chain 3; MTOR: mechanistic target of rapamycin kinase; NCBI: national center for biotechnology information; ORF: open reading frame; PBS: phosphate-buffered saline; PCR: polymerase chain reaction; PtdIns3K: class III phosphatidylinositol 3-kinase; RACE-PCR: rapid amplification of cDNA-ends by polymerase chain reaction; RNA: ribonucleic acid; SQSTM1: sequestosome 1; Uniprot: universal protein resource; WIPI: WD repeat domain, phosphoinositide interacting. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Autophagy 16 11 2017 2035 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Autophagy autophagy related crassostrea gigas data mining immunohistochemistry real-time PCR western blot |
spellingShingle |
Autophagy autophagy related crassostrea gigas data mining immunohistochemistry real-time PCR western blot Picot, Sandy Faury, Nicole Arzul, Isabelle Chollet, Bruno Renault, Tristan Morga, Benjamin Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas |
topic_facet |
Autophagy autophagy related crassostrea gigas data mining immunohistochemistry real-time PCR western blot |
description |
The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism elimination. Autophagy also exists in C. gigas, and plays a role in the immune response against infections. Although this process is well-documented and conserved in most animals, it is still poorly understood in mollusks. To date, no study has provided a complete overview of the molecular mechanism of autophagy in mollusk bivalves. In this study, human and yeast ATG protein sequences and public databases (Uniprot and NCBI) were used to identify protein members of the C. gigas autophagy pathway. A total of 35 autophagy related proteins were found in the Pacific oyster. RACE-PCR was performed on several genes. Using molecular (real-time PCR) and protein-based (western blot and immunohistochemistry) approaches, the expression and localization of ATG12, ATG9, BECN1, MAP1LC3, MTOR, and SQSTM1, was investigated in different tissues of the Pacific oyster. Comparison with human and yeast counterparts demonstrated a high homology with the human autophagy pathway. The results also demonstrated that the key autophagy genes and their protein products were expressed in all the analyzed tissues of C. gigas. This study allows the characterization of the complete C. gigas autophagy pathway for the first time. Abbreviations: ATG: autophagy related; Atg1/ULK: unc-51 like autophagy activating kinase; ATG7: autophagy related 7; ATG9: autophagy related 9; ATG12: autophagy related 12; BECN1: beclin 1; BSA: bovine serum albumin; cDNA: complementary deoxyribonucleic acid; DNA: deoxyribonucleic acid; GABARAP: GABA type A receptor-associated protein; IHC: immunohistochemistry; MAP1LC3/LC3/Atg8: microtubule associated protein 1 light chain 3; MTOR: mechanistic target of rapamycin kinase; NCBI: national center for biotechnology information; ORF: open reading frame; PBS: phosphate-buffered saline; PCR: polymerase chain reaction; PtdIns3K: class III phosphatidylinositol 3-kinase; RACE-PCR: rapid amplification of cDNA-ends by polymerase chain reaction; RNA: ribonucleic acid; SQSTM1: sequestosome 1; Uniprot: universal protein resource; WIPI: WD repeat domain, phosphoinositide interacting. |
format |
Article in Journal/Newspaper |
author |
Picot, Sandy Faury, Nicole Arzul, Isabelle Chollet, Bruno Renault, Tristan Morga, Benjamin |
author_facet |
Picot, Sandy Faury, Nicole Arzul, Isabelle Chollet, Bruno Renault, Tristan Morga, Benjamin |
author_sort |
Picot, Sandy |
title |
Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas |
title_short |
Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas |
title_full |
Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas |
title_fullStr |
Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas |
title_full_unstemmed |
Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas |
title_sort |
identification of the autophagy pathway in a mollusk bivalve, crassostrea gigas |
publisher |
Informa UK Limited |
publishDate |
2020 |
url |
https://archimer.ifremer.fr/doc/00606/71785/70270.pdf https://archimer.ifremer.fr/doc/00606/71785/70271.pdf https://archimer.ifremer.fr/doc/00606/71785/70272.pdf https://archimer.ifremer.fr/doc/00606/71785/70273.pdf https://archimer.ifremer.fr/doc/00606/71785/70274.pdf https://doi.org/10.1080/15548627.2020.1713643 https://archimer.ifremer.fr/doc/00606/71785/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Autophagy (1554-8627) (Informa UK Limited), 2020-11 , Vol. 16 , N. 11 , P. 2017-2035 |
op_relation |
https://archimer.ifremer.fr/doc/00606/71785/70270.pdf https://archimer.ifremer.fr/doc/00606/71785/70271.pdf https://archimer.ifremer.fr/doc/00606/71785/70272.pdf https://archimer.ifremer.fr/doc/00606/71785/70273.pdf https://archimer.ifremer.fr/doc/00606/71785/70274.pdf doi:10.1080/15548627.2020.1713643 https://archimer.ifremer.fr/doc/00606/71785/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1080/15548627.2020.1713643 |
container_title |
Autophagy |
container_volume |
16 |
container_issue |
11 |
container_start_page |
2017 |
op_container_end_page |
2035 |
_version_ |
1766393775422177280 |