A Large-Scale Epidemiological Study to Identify Bacteria Pathogenic to Pacific Oyster Crassostrea gigas and Correlation Between Virulence and Metalloprotease-like Activity
A 4-year bacteriological survey (2003-2007) of four molluscs cultivated in France and faced with mortality episodes was performed by the French shellfish pathology network. The more abundant bacteria isolated during 92 mortality episodes, occurring mainly in Pacific oyster Crassostrea gigas, were id...
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Online Access: | https://archimer.ifremer.fr/doc/2009/publication-6974.pdf https://doi.org/10.1007/s00248-009-9620-y https://archimer.ifremer.fr/doc/00000/6974/ |
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ftarchimer:oai:archimer.ifremer.fr:6974 2023-05-15T15:58:01+02:00 A Large-Scale Epidemiological Study to Identify Bacteria Pathogenic to Pacific Oyster Crassostrea gigas and Correlation Between Virulence and Metalloprotease-like Activity Saulnier, Denis De Decker, Sophie Haffner, Philippe Cobret, Laetitia Robert, Maeva Garcia, Celine 2010-05 application/pdf https://archimer.ifremer.fr/doc/2009/publication-6974.pdf https://doi.org/10.1007/s00248-009-9620-y https://archimer.ifremer.fr/doc/00000/6974/ eng eng Springer https://archimer.ifremer.fr/doc/2009/publication-6974.pdf doi:10.1007/s00248-009-9620-y https://archimer.ifremer.fr/doc/00000/6974/ 2010 Springer. Part of Springer Science+Business Media info:eu-repo/semantics/openAccess restricted use Microbial Ecology (0095-3628) (Springer), 2010-05 , Vol. 59 , N. 4 , P. 787-798 text Publication info:eu-repo/semantics/article 2010 ftarchimer https://doi.org/10.1007/s00248-009-9620-y 2021-09-23T20:17:39Z A 4-year bacteriological survey (2003-2007) of four molluscs cultivated in France and faced with mortality episodes was performed by the French shellfish pathology network. The more abundant bacteria isolated during 92 mortality episodes, occurring mainly in Pacific oyster Crassostrea gigas, were identified by genotyping methods. It allowed us both to confirm the representativeness of Vibrio splendidus and Vibrio aestuarianus bacterial strains and to identify both a large number of Vibrio harveyi-related strains mainly detected during 2007 oyster mortality outbreaks and to a lesser extent bacterial strains identified as Shewanella colwelliana. Because metalloprotease has been reported to constitute a virulence factor in a few Vibrio strains pathogenic for C. gigas, several bacterial strains isolated in this study were screened to evaluate their pathogenicity in C. gigas spat by experimental infection and their ability to produce metalloprotease-like activity in the culture supernatant fluids. A high level (84%) of concordant results between azocaseinase activities and virulence of strains was obtained in this study. Because bacterial metalloprotease activities appeared as a common feature of pathogenic bacteria strains associated with mortality events of C. gigas reared in France, this phenotypic test could be useful for the evaluation of virulence in bacterial strains associated with such mortality episodes. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Microbial Ecology 59 4 787 798 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
description |
A 4-year bacteriological survey (2003-2007) of four molluscs cultivated in France and faced with mortality episodes was performed by the French shellfish pathology network. The more abundant bacteria isolated during 92 mortality episodes, occurring mainly in Pacific oyster Crassostrea gigas, were identified by genotyping methods. It allowed us both to confirm the representativeness of Vibrio splendidus and Vibrio aestuarianus bacterial strains and to identify both a large number of Vibrio harveyi-related strains mainly detected during 2007 oyster mortality outbreaks and to a lesser extent bacterial strains identified as Shewanella colwelliana. Because metalloprotease has been reported to constitute a virulence factor in a few Vibrio strains pathogenic for C. gigas, several bacterial strains isolated in this study were screened to evaluate their pathogenicity in C. gigas spat by experimental infection and their ability to produce metalloprotease-like activity in the culture supernatant fluids. A high level (84%) of concordant results between azocaseinase activities and virulence of strains was obtained in this study. Because bacterial metalloprotease activities appeared as a common feature of pathogenic bacteria strains associated with mortality events of C. gigas reared in France, this phenotypic test could be useful for the evaluation of virulence in bacterial strains associated with such mortality episodes. |
format |
Article in Journal/Newspaper |
author |
Saulnier, Denis De Decker, Sophie Haffner, Philippe Cobret, Laetitia Robert, Maeva Garcia, Celine |
spellingShingle |
Saulnier, Denis De Decker, Sophie Haffner, Philippe Cobret, Laetitia Robert, Maeva Garcia, Celine A Large-Scale Epidemiological Study to Identify Bacteria Pathogenic to Pacific Oyster Crassostrea gigas and Correlation Between Virulence and Metalloprotease-like Activity |
author_facet |
Saulnier, Denis De Decker, Sophie Haffner, Philippe Cobret, Laetitia Robert, Maeva Garcia, Celine |
author_sort |
Saulnier, Denis |
title |
A Large-Scale Epidemiological Study to Identify Bacteria Pathogenic to Pacific Oyster Crassostrea gigas and Correlation Between Virulence and Metalloprotease-like Activity |
title_short |
A Large-Scale Epidemiological Study to Identify Bacteria Pathogenic to Pacific Oyster Crassostrea gigas and Correlation Between Virulence and Metalloprotease-like Activity |
title_full |
A Large-Scale Epidemiological Study to Identify Bacteria Pathogenic to Pacific Oyster Crassostrea gigas and Correlation Between Virulence and Metalloprotease-like Activity |
title_fullStr |
A Large-Scale Epidemiological Study to Identify Bacteria Pathogenic to Pacific Oyster Crassostrea gigas and Correlation Between Virulence and Metalloprotease-like Activity |
title_full_unstemmed |
A Large-Scale Epidemiological Study to Identify Bacteria Pathogenic to Pacific Oyster Crassostrea gigas and Correlation Between Virulence and Metalloprotease-like Activity |
title_sort |
large-scale epidemiological study to identify bacteria pathogenic to pacific oyster crassostrea gigas and correlation between virulence and metalloprotease-like activity |
publisher |
Springer |
publishDate |
2010 |
url |
https://archimer.ifremer.fr/doc/2009/publication-6974.pdf https://doi.org/10.1007/s00248-009-9620-y https://archimer.ifremer.fr/doc/00000/6974/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Microbial Ecology (0095-3628) (Springer), 2010-05 , Vol. 59 , N. 4 , P. 787-798 |
op_relation |
https://archimer.ifremer.fr/doc/2009/publication-6974.pdf doi:10.1007/s00248-009-9620-y https://archimer.ifremer.fr/doc/00000/6974/ |
op_rights |
2010 Springer. Part of Springer Science+Business Media info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1007/s00248-009-9620-y |
container_title |
Microbial Ecology |
container_volume |
59 |
container_issue |
4 |
container_start_page |
787 |
op_container_end_page |
798 |
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1766393735520714752 |