Detection of oyster herpesvirus DNA and proteins in asymptomatic Crassostrea gigas adults
Since 1972, several herpes-like virus infections have been reported among different bivalve species around the world. Most of these reports involved larvae or juveniles presenting high mortalities. Two case reports of herpes-like viruses concerned adult oysters, Crassostrea virginica in USA and Ostr...
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ftarchimer:oai:archimer.ifremer.fr:697 2023-05-15T15:57:37+02:00 Detection of oyster herpesvirus DNA and proteins in asymptomatic Crassostrea gigas adults Arzul, Isabelle Renault, Tristan Thebault, Anne Gerard, Andre 2002-03 application/pdf https://archimer.ifremer.fr/doc/2002/publication-697.pdf https://doi.org/10.1016/S0168-1702(02)00007-2 https://archimer.ifremer.fr/doc/00000/697/ eng eng Elsevier https://archimer.ifremer.fr/doc/2002/publication-697.pdf doi:10.1016/S0168-1702(02)00007-2 https://archimer.ifremer.fr/doc/00000/697/ 2002 Elsevier Science B.V. All rights reserved info:eu-repo/semantics/openAccess restricted use Virus Research (0168-1702) (Elsevier), 2002-03 , Vol. 84 , N. 1-2 , P. 151-160 Protein DNA Healthy carrier Adult oyster Crassostrea gigas Herpesvirus text Publication info:eu-repo/semantics/article 2002 ftarchimer https://doi.org/10.1016/S0168-1702(02)00007-2 2021-09-23T20:13:16Z Since 1972, several herpes-like virus infections have been reported among different bivalve species around the world. Most of these reports involved larvae or juveniles presenting high mortalities. Two case reports of herpes-like viruses concerned adult oysters, Crassostrea virginica in USA and Ostrea angasi in Australia. Molecular techniques including PCR and in situ hybridization (ISH) have been recently developed to detect the oyster herpesvirus genome. In the present study, 30 Pacific oyster, Crassostrea gigas, adults have been analyzed using three different techniques: PCR, ISH and immunochemistry, in order to detect herpesviruses in asymptomatic individuals. PCR and ISH allowed detection of oyster herpesvirus DNA in 93.3 and 86.6%, respectively, of analyzed oysters while polyclonal antibodies allowed detection of viral proteins in 76.6% of analyzed adult oysters. These results suggest that oyster herpesvirus infects adult oysters with high prevalence and that the virus may persist in its host after primary infection. The detection of viral DNA and viral proteins in the gonad of several individuals supports the hypothesis of a possible vertical transmission of the infection. Lastly, concordance among the three techniques used in this study is discussed. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Virus Research 84 1-2 151 160 |
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Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Protein DNA Healthy carrier Adult oyster Crassostrea gigas Herpesvirus |
spellingShingle |
Protein DNA Healthy carrier Adult oyster Crassostrea gigas Herpesvirus Arzul, Isabelle Renault, Tristan Thebault, Anne Gerard, Andre Detection of oyster herpesvirus DNA and proteins in asymptomatic Crassostrea gigas adults |
topic_facet |
Protein DNA Healthy carrier Adult oyster Crassostrea gigas Herpesvirus |
description |
Since 1972, several herpes-like virus infections have been reported among different bivalve species around the world. Most of these reports involved larvae or juveniles presenting high mortalities. Two case reports of herpes-like viruses concerned adult oysters, Crassostrea virginica in USA and Ostrea angasi in Australia. Molecular techniques including PCR and in situ hybridization (ISH) have been recently developed to detect the oyster herpesvirus genome. In the present study, 30 Pacific oyster, Crassostrea gigas, adults have been analyzed using three different techniques: PCR, ISH and immunochemistry, in order to detect herpesviruses in asymptomatic individuals. PCR and ISH allowed detection of oyster herpesvirus DNA in 93.3 and 86.6%, respectively, of analyzed oysters while polyclonal antibodies allowed detection of viral proteins in 76.6% of analyzed adult oysters. These results suggest that oyster herpesvirus infects adult oysters with high prevalence and that the virus may persist in its host after primary infection. The detection of viral DNA and viral proteins in the gonad of several individuals supports the hypothesis of a possible vertical transmission of the infection. Lastly, concordance among the three techniques used in this study is discussed. |
format |
Article in Journal/Newspaper |
author |
Arzul, Isabelle Renault, Tristan Thebault, Anne Gerard, Andre |
author_facet |
Arzul, Isabelle Renault, Tristan Thebault, Anne Gerard, Andre |
author_sort |
Arzul, Isabelle |
title |
Detection of oyster herpesvirus DNA and proteins in asymptomatic Crassostrea gigas adults |
title_short |
Detection of oyster herpesvirus DNA and proteins in asymptomatic Crassostrea gigas adults |
title_full |
Detection of oyster herpesvirus DNA and proteins in asymptomatic Crassostrea gigas adults |
title_fullStr |
Detection of oyster herpesvirus DNA and proteins in asymptomatic Crassostrea gigas adults |
title_full_unstemmed |
Detection of oyster herpesvirus DNA and proteins in asymptomatic Crassostrea gigas adults |
title_sort |
detection of oyster herpesvirus dna and proteins in asymptomatic crassostrea gigas adults |
publisher |
Elsevier |
publishDate |
2002 |
url |
https://archimer.ifremer.fr/doc/2002/publication-697.pdf https://doi.org/10.1016/S0168-1702(02)00007-2 https://archimer.ifremer.fr/doc/00000/697/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Virus Research (0168-1702) (Elsevier), 2002-03 , Vol. 84 , N. 1-2 , P. 151-160 |
op_relation |
https://archimer.ifremer.fr/doc/2002/publication-697.pdf doi:10.1016/S0168-1702(02)00007-2 https://archimer.ifremer.fr/doc/00000/697/ |
op_rights |
2002 Elsevier Science B.V. All rights reserved info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/S0168-1702(02)00007-2 |
container_title |
Virus Research |
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84 |
container_issue |
1-2 |
container_start_page |
151 |
op_container_end_page |
160 |
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1766393289679831040 |