Molecular identification and expression of two non-P450 enzymes, monoamine oxidase A and flavin-containing monooxygenase 2, involved in phase I of xenobiotic biotransformation in the Pacific oyster, Crassostrea gigas
Marine bivalve metabolism can be perturbed by hydrocarbon and pesticide pollution in coastal ecosystems. In this study, in the Pacific oyster, Crassostrea gigas, full-length cDNAs encoding two non-P450 phase I enzymes, flavin-containing monooxygenase 2 (FMO-2) and monamine oxidase A (MAO A), were ch...
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ftarchimer:oai:archimer.ifremer.fr:676 2023-05-15T15:57:42+02:00 Molecular identification and expression of two non-P450 enzymes, monoamine oxidase A and flavin-containing monooxygenase 2, involved in phase I of xenobiotic biotransformation in the Pacific oyster, Crassostrea gigas Boutet, Isabelle Tanguy, Aurelie Moraga, Dario 2004-07 application/pdf https://archimer.ifremer.fr/doc/2004/publication-676.pdf https://doi.org/10.1016/j.bbaexp.2004.04.001 https://archimer.ifremer.fr/doc/00000/676/ eng eng Elsevier https://archimer.ifremer.fr/doc/2004/publication-676.pdf doi:10.1016/j.bbaexp.2004.04.001 https://archimer.ifremer.fr/doc/00000/676/ 2004 Elsevier B.V. All rights reserved info:eu-repo/semantics/openAccess restricted use Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression (0167-4781) (Elsevier), 2004-07 , Vol. 1679 , N. 1 , P. 29-36 Crassostrea gigas Flavin containing monooxygenase 2 Monoamine oxidase A text Publication info:eu-repo/semantics/article 2004 ftarchimer https://doi.org/10.1016/j.bbaexp.2004.04.001 2021-09-23T20:13:16Z Marine bivalve metabolism can be perturbed by hydrocarbon and pesticide pollution in coastal ecosystems. In this study, in the Pacific oyster, Crassostrea gigas, full-length cDNAs encoding two non-P450 phase I enzymes, flavin-containing monooxygenase 2 (FMO-2) and monamine oxidase A (MAO A), were characterized. Both sequences contained the co-factor fixation motifs characteristic of their respective enzyme families. Using reverse transcription polymerase chain reaction (RT-PCR), the messenger RNA (mRNA) transcription levels of these two enzymes in tissues of oysters exposed, under experimental conditions, to hydrocarbons and two pesticide treatments were investigated. The pesticide treatments were exposure to either glyphosate or to a mixture composed of atrazine, diuron and isoproturon. The results showed a strong differential expression of FMO-2 and MAO A that was both tissue-specific as well as time- and treatment-dependent. It was also clearly demonstrated that the transcription levels of MAO A (generally considered a constitutive enzyme without external regulation) were induced by hydrocarbons and pesticides in digestive gland and inhibited by pesticides in gill tissue. Furthermore, the transcription levels of FMO-2 and MAO A mRNA in digestive gland might be useful as a marker of hydrocarbon or pesticide exposure in monitoring programs. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression 1679 1 29 36 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Crassostrea gigas Flavin containing monooxygenase 2 Monoamine oxidase A |
spellingShingle |
Crassostrea gigas Flavin containing monooxygenase 2 Monoamine oxidase A Boutet, Isabelle Tanguy, Aurelie Moraga, Dario Molecular identification and expression of two non-P450 enzymes, monoamine oxidase A and flavin-containing monooxygenase 2, involved in phase I of xenobiotic biotransformation in the Pacific oyster, Crassostrea gigas |
topic_facet |
Crassostrea gigas Flavin containing monooxygenase 2 Monoamine oxidase A |
description |
Marine bivalve metabolism can be perturbed by hydrocarbon and pesticide pollution in coastal ecosystems. In this study, in the Pacific oyster, Crassostrea gigas, full-length cDNAs encoding two non-P450 phase I enzymes, flavin-containing monooxygenase 2 (FMO-2) and monamine oxidase A (MAO A), were characterized. Both sequences contained the co-factor fixation motifs characteristic of their respective enzyme families. Using reverse transcription polymerase chain reaction (RT-PCR), the messenger RNA (mRNA) transcription levels of these two enzymes in tissues of oysters exposed, under experimental conditions, to hydrocarbons and two pesticide treatments were investigated. The pesticide treatments were exposure to either glyphosate or to a mixture composed of atrazine, diuron and isoproturon. The results showed a strong differential expression of FMO-2 and MAO A that was both tissue-specific as well as time- and treatment-dependent. It was also clearly demonstrated that the transcription levels of MAO A (generally considered a constitutive enzyme without external regulation) were induced by hydrocarbons and pesticides in digestive gland and inhibited by pesticides in gill tissue. Furthermore, the transcription levels of FMO-2 and MAO A mRNA in digestive gland might be useful as a marker of hydrocarbon or pesticide exposure in monitoring programs. |
format |
Article in Journal/Newspaper |
author |
Boutet, Isabelle Tanguy, Aurelie Moraga, Dario |
author_facet |
Boutet, Isabelle Tanguy, Aurelie Moraga, Dario |
author_sort |
Boutet, Isabelle |
title |
Molecular identification and expression of two non-P450 enzymes, monoamine oxidase A and flavin-containing monooxygenase 2, involved in phase I of xenobiotic biotransformation in the Pacific oyster, Crassostrea gigas |
title_short |
Molecular identification and expression of two non-P450 enzymes, monoamine oxidase A and flavin-containing monooxygenase 2, involved in phase I of xenobiotic biotransformation in the Pacific oyster, Crassostrea gigas |
title_full |
Molecular identification and expression of two non-P450 enzymes, monoamine oxidase A and flavin-containing monooxygenase 2, involved in phase I of xenobiotic biotransformation in the Pacific oyster, Crassostrea gigas |
title_fullStr |
Molecular identification and expression of two non-P450 enzymes, monoamine oxidase A and flavin-containing monooxygenase 2, involved in phase I of xenobiotic biotransformation in the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Molecular identification and expression of two non-P450 enzymes, monoamine oxidase A and flavin-containing monooxygenase 2, involved in phase I of xenobiotic biotransformation in the Pacific oyster, Crassostrea gigas |
title_sort |
molecular identification and expression of two non-p450 enzymes, monoamine oxidase a and flavin-containing monooxygenase 2, involved in phase i of xenobiotic biotransformation in the pacific oyster, crassostrea gigas |
publisher |
Elsevier |
publishDate |
2004 |
url |
https://archimer.ifremer.fr/doc/2004/publication-676.pdf https://doi.org/10.1016/j.bbaexp.2004.04.001 https://archimer.ifremer.fr/doc/00000/676/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression (0167-4781) (Elsevier), 2004-07 , Vol. 1679 , N. 1 , P. 29-36 |
op_relation |
https://archimer.ifremer.fr/doc/2004/publication-676.pdf doi:10.1016/j.bbaexp.2004.04.001 https://archimer.ifremer.fr/doc/00000/676/ |
op_rights |
2004 Elsevier B.V. All rights reserved info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.bbaexp.2004.04.001 |
container_title |
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression |
container_volume |
1679 |
container_issue |
1 |
container_start_page |
29 |
op_container_end_page |
36 |
_version_ |
1766393391626584064 |