In vivo RNA interference in oyster -vasa silencing inhibits germ cell development
This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In vivo injecti...
Published in: | FEBS Journal |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley / Blackwell
2009
|
Subjects: | |
Online Access: | https://archimer.ifremer.fr/doc/2009/publication-6641.pdf https://doi.org/10.1111/j.1742-4658.2009.06982.x https://archimer.ifremer.fr/doc/00000/6641/ |
id |
ftarchimer:oai:archimer.ifremer.fr:6641 |
---|---|
record_format |
openpolar |
spelling |
ftarchimer:oai:archimer.ifremer.fr:6641 2023-05-15T15:57:27+02:00 In vivo RNA interference in oyster -vasa silencing inhibits germ cell development Fabioux, Caroline Corporeau, Charlotte Quillien, Virgile Favrel, Pascal Huvet, Arnaud 2009-05 application/pdf https://archimer.ifremer.fr/doc/2009/publication-6641.pdf https://doi.org/10.1111/j.1742-4658.2009.06982.x https://archimer.ifremer.fr/doc/00000/6641/ eng eng Wiley / Blackwell https://archimer.ifremer.fr/doc/2009/publication-6641.pdf doi:10.1111/j.1742-4658.2009.06982.x https://archimer.ifremer.fr/doc/00000/6641/ 2009 Wiley Blackwell, Federation of European Biochemical Societies info:eu-repo/semantics/openAccess restricted use FEBS Journal (1742-464X) (Wiley / Blackwell), 2009-05 , Vol. 276 , N. 9 , P. 2566-2573 Vasa RNAi Marine bivalve Germline Crassostrea gigas text Publication info:eu-repo/semantics/article 2009 ftarchimer https://doi.org/10.1111/j.1742-4658.2009.06982.x 2021-09-23T20:17:24Z This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In vivo injection of oyvl-dsRNA into the gonad provokes a knockdown phenotype corresponding to germ cell underproliferation and prematurely arrested meiosis througout the organ. The most severe phenotype observed is sterile. This knockdown phenotype is associated with a decrease in Oyvlg mRNA level of between 39% and 87%, and a strong reduction in OYVLG protein, to an undetectable level. Therefore, Oyvlg appears to be essential for germ cell development in Crassostrea gigas, particularly for mitotic proliferation and early meiosis. Our results demonstrate for the first time that in vivo RNA interference works efficiently in a bivalve species, opening major perspectives for functional genetic studies. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Vasa ENVELOPE(25.177,25.177,67.587,67.587) FEBS Journal 276 9 2566 2573 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Vasa RNAi Marine bivalve Germline Crassostrea gigas |
spellingShingle |
Vasa RNAi Marine bivalve Germline Crassostrea gigas Fabioux, Caroline Corporeau, Charlotte Quillien, Virgile Favrel, Pascal Huvet, Arnaud In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
topic_facet |
Vasa RNAi Marine bivalve Germline Crassostrea gigas |
description |
This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In vivo injection of oyvl-dsRNA into the gonad provokes a knockdown phenotype corresponding to germ cell underproliferation and prematurely arrested meiosis througout the organ. The most severe phenotype observed is sterile. This knockdown phenotype is associated with a decrease in Oyvlg mRNA level of between 39% and 87%, and a strong reduction in OYVLG protein, to an undetectable level. Therefore, Oyvlg appears to be essential for germ cell development in Crassostrea gigas, particularly for mitotic proliferation and early meiosis. Our results demonstrate for the first time that in vivo RNA interference works efficiently in a bivalve species, opening major perspectives for functional genetic studies. |
format |
Article in Journal/Newspaper |
author |
Fabioux, Caroline Corporeau, Charlotte Quillien, Virgile Favrel, Pascal Huvet, Arnaud |
author_facet |
Fabioux, Caroline Corporeau, Charlotte Quillien, Virgile Favrel, Pascal Huvet, Arnaud |
author_sort |
Fabioux, Caroline |
title |
In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
title_short |
In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
title_full |
In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
title_fullStr |
In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
title_full_unstemmed |
In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
title_sort |
in vivo rna interference in oyster -vasa silencing inhibits germ cell development |
publisher |
Wiley / Blackwell |
publishDate |
2009 |
url |
https://archimer.ifremer.fr/doc/2009/publication-6641.pdf https://doi.org/10.1111/j.1742-4658.2009.06982.x https://archimer.ifremer.fr/doc/00000/6641/ |
long_lat |
ENVELOPE(25.177,25.177,67.587,67.587) |
geographic |
Vasa |
geographic_facet |
Vasa |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
FEBS Journal (1742-464X) (Wiley / Blackwell), 2009-05 , Vol. 276 , N. 9 , P. 2566-2573 |
op_relation |
https://archimer.ifremer.fr/doc/2009/publication-6641.pdf doi:10.1111/j.1742-4658.2009.06982.x https://archimer.ifremer.fr/doc/00000/6641/ |
op_rights |
2009 Wiley Blackwell, Federation of European Biochemical Societies info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1111/j.1742-4658.2009.06982.x |
container_title |
FEBS Journal |
container_volume |
276 |
container_issue |
9 |
container_start_page |
2566 |
op_container_end_page |
2573 |
_version_ |
1766393107028377600 |