Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas
The oyster vasa-like gene was previously demonstrated to be specifically expressed in germline cells of adult oysters Crassostrea gigas. In the present study, this gene was used as a molecular marker to establish the developmental pattern of germline cells during oyster ontogenesis. using whole-moun...
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ftarchimer:oai:archimer.ifremer.fr:648 2023-05-15T15:57:35+02:00 Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas Fabioux, Caroline Huvet, Arnaud Lelong, C Robert, Rene Pouvreau, Stephane Daniel, Jean-yves Mingant, Christian Le Pennec, M 2004-07 application/pdf https://archimer.ifremer.fr/doc/2004/publication-648.pdf https://doi.org/10.1016/j.bbrc.2004.06.009 https://archimer.ifremer.fr/doc/00000/648/ eng eng Elsevier https://archimer.ifremer.fr/doc/2004/publication-648.pdf doi:10.1016/j.bbrc.2004.06.009 https://archimer.ifremer.fr/doc/00000/648/ 2004 Elsevier Inc. All rights reserved info:eu-repo/semantics/openAccess restricted use Biochemical and Biophysical Research Communications (0006-291X) (Elsevier), 2004-07 , Vol. 320 , N. 2 , P. 592-598 Primordial germ cells Oyster vasa like gene Germline Development Crassostrea gigas text Publication info:eu-repo/semantics/article 2004 ftarchimer https://doi.org/10.1016/j.bbrc.2004.06.009 2021-09-23T20:13:16Z The oyster vasa-like gene was previously demonstrated to be specifically expressed in germline cells of adult oysters Crassostrea gigas. In the present study, this gene was used as a molecular marker to establish the developmental pattern of germline cells during oyster ontogenesis. using whole-mount in situ hybridization and real-time PCR. The Oyvlg transcripts appeared to be localized to the vegetal pole of unfertilized oocytes and maternally transmitted to embryos. At early development, these maternal transcripts were observed to segregate into a single blastomere, from the CD macromere of 2-cell stage to the 4d mesentoblast of blastula. From late blastula stage, the mesentoblast divided into two cell clumps that migrated to both sides of the larvae body and that would correspond to primordial germ cells (PGCs). Based on these results, we postulate that the germline of C. gigas is specified at early development by maternal cytoplasmic determinants including Oyvlg mRNAs, in putative PGCs that would differentiate into germinal stem cells in juvenile oysters. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Vasa ENVELOPE(25.177,25.177,67.587,67.587) Biochemical and Biophysical Research Communications 320 2 592 598 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Primordial germ cells Oyster vasa like gene Germline Development Crassostrea gigas |
spellingShingle |
Primordial germ cells Oyster vasa like gene Germline Development Crassostrea gigas Fabioux, Caroline Huvet, Arnaud Lelong, C Robert, Rene Pouvreau, Stephane Daniel, Jean-yves Mingant, Christian Le Pennec, M Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas |
topic_facet |
Primordial germ cells Oyster vasa like gene Germline Development Crassostrea gigas |
description |
The oyster vasa-like gene was previously demonstrated to be specifically expressed in germline cells of adult oysters Crassostrea gigas. In the present study, this gene was used as a molecular marker to establish the developmental pattern of germline cells during oyster ontogenesis. using whole-mount in situ hybridization and real-time PCR. The Oyvlg transcripts appeared to be localized to the vegetal pole of unfertilized oocytes and maternally transmitted to embryos. At early development, these maternal transcripts were observed to segregate into a single blastomere, from the CD macromere of 2-cell stage to the 4d mesentoblast of blastula. From late blastula stage, the mesentoblast divided into two cell clumps that migrated to both sides of the larvae body and that would correspond to primordial germ cells (PGCs). Based on these results, we postulate that the germline of C. gigas is specified at early development by maternal cytoplasmic determinants including Oyvlg mRNAs, in putative PGCs that would differentiate into germinal stem cells in juvenile oysters. |
format |
Article in Journal/Newspaper |
author |
Fabioux, Caroline Huvet, Arnaud Lelong, C Robert, Rene Pouvreau, Stephane Daniel, Jean-yves Mingant, Christian Le Pennec, M |
author_facet |
Fabioux, Caroline Huvet, Arnaud Lelong, C Robert, Rene Pouvreau, Stephane Daniel, Jean-yves Mingant, Christian Le Pennec, M |
author_sort |
Fabioux, Caroline |
title |
Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas |
title_short |
Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas |
title_full |
Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas |
title_fullStr |
Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas |
title_full_unstemmed |
Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas |
title_sort |
oyster vasa-like gene as a marker of the germline cell development in crassostrea gigas |
publisher |
Elsevier |
publishDate |
2004 |
url |
https://archimer.ifremer.fr/doc/2004/publication-648.pdf https://doi.org/10.1016/j.bbrc.2004.06.009 https://archimer.ifremer.fr/doc/00000/648/ |
long_lat |
ENVELOPE(25.177,25.177,67.587,67.587) |
geographic |
Vasa |
geographic_facet |
Vasa |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Biochemical and Biophysical Research Communications (0006-291X) (Elsevier), 2004-07 , Vol. 320 , N. 2 , P. 592-598 |
op_relation |
https://archimer.ifremer.fr/doc/2004/publication-648.pdf doi:10.1016/j.bbrc.2004.06.009 https://archimer.ifremer.fr/doc/00000/648/ |
op_rights |
2004 Elsevier Inc. All rights reserved info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.bbrc.2004.06.009 |
container_title |
Biochemical and Biophysical Research Communications |
container_volume |
320 |
container_issue |
2 |
container_start_page |
592 |
op_container_end_page |
598 |
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1766393255929315328 |