The oyster vasa-like gene: a specific marker of the germline in Crassostrea gigas
The vasa gene is,a key determinant for germline formation in eukaryotes. This gene, highly conserved through evolution, encodes a RNA helicase protein member of the DEAD-box family. To understand the germline formation in oyster, we report here the isolation and the characterization of a vasa orthol...
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ftarchimer:oai:archimer.ifremer.fr:646 2023-05-15T15:57:48+02:00 The oyster vasa-like gene: a specific marker of the germline in Crassostrea gigas Fabioux, Caroline Pouvreau, Stephane Leroux, Frederique Huvet, Arnaud 2004-03 application/pdf https://archimer.ifremer.fr/doc/2004/publication-646.pdf https://doi.org/10.1016/j.bbrc.2004.01.145 https://archimer.ifremer.fr/doc/00000/646/ eng eng Elsevier https://archimer.ifremer.fr/doc/2004/publication-646.pdf doi:10.1016/j.bbrc.2004.01.145 https://archimer.ifremer.fr/doc/00000/646/ 2004 Elsevier Inc. All rights reserved info:eu-repo/semantics/openAccess restricted use Biochemical and Biophysical Research Communications (0006-291X) (Elsevier), 2004-03 , Vol. 315 , N. 4 , P. 897-904 RNA helicase DEAD box Vasa Germline Gametogenesis Oyster text Publication info:eu-repo/semantics/article 2004 ftarchimer https://doi.org/10.1016/j.bbrc.2004.01.145 2021-09-23T20:13:16Z The vasa gene is,a key determinant for germline formation in eukaryotes. This gene, highly conserved through evolution, encodes a RNA helicase protein member of the DEAD-box family. To understand the germline formation in oyster, we report here the isolation and the characterization of a vasa orthologue in Crassostrea gigas (Oyvlg). OyVLG contained the eight consensus domains of the DEAD-box including those providing RNA unwinding activity. The expression pattern of Oyvlg was examined in adult oyster tissues at different reproductive stages. Its expression was restricted to germline cells both in males and females, including germinal stem cells and auxiliary cells. The expression of Oyvlg, strongest in early germ cells, decreased as the maturation proceeded. These data and the evolutionary conservation observed suggested the role of Oyvlg in germline development. Oyvlg is the first germ cell specific marker in oyster and will be very useful in studies of oyster germline formation. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Vasa ENVELOPE(25.177,25.177,67.587,67.587) Biochemical and Biophysical Research Communications 315 4 897 904 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
RNA helicase DEAD box Vasa Germline Gametogenesis Oyster |
spellingShingle |
RNA helicase DEAD box Vasa Germline Gametogenesis Oyster Fabioux, Caroline Pouvreau, Stephane Leroux, Frederique Huvet, Arnaud The oyster vasa-like gene: a specific marker of the germline in Crassostrea gigas |
topic_facet |
RNA helicase DEAD box Vasa Germline Gametogenesis Oyster |
description |
The vasa gene is,a key determinant for germline formation in eukaryotes. This gene, highly conserved through evolution, encodes a RNA helicase protein member of the DEAD-box family. To understand the germline formation in oyster, we report here the isolation and the characterization of a vasa orthologue in Crassostrea gigas (Oyvlg). OyVLG contained the eight consensus domains of the DEAD-box including those providing RNA unwinding activity. The expression pattern of Oyvlg was examined in adult oyster tissues at different reproductive stages. Its expression was restricted to germline cells both in males and females, including germinal stem cells and auxiliary cells. The expression of Oyvlg, strongest in early germ cells, decreased as the maturation proceeded. These data and the evolutionary conservation observed suggested the role of Oyvlg in germline development. Oyvlg is the first germ cell specific marker in oyster and will be very useful in studies of oyster germline formation. |
format |
Article in Journal/Newspaper |
author |
Fabioux, Caroline Pouvreau, Stephane Leroux, Frederique Huvet, Arnaud |
author_facet |
Fabioux, Caroline Pouvreau, Stephane Leroux, Frederique Huvet, Arnaud |
author_sort |
Fabioux, Caroline |
title |
The oyster vasa-like gene: a specific marker of the germline in Crassostrea gigas |
title_short |
The oyster vasa-like gene: a specific marker of the germline in Crassostrea gigas |
title_full |
The oyster vasa-like gene: a specific marker of the germline in Crassostrea gigas |
title_fullStr |
The oyster vasa-like gene: a specific marker of the germline in Crassostrea gigas |
title_full_unstemmed |
The oyster vasa-like gene: a specific marker of the germline in Crassostrea gigas |
title_sort |
oyster vasa-like gene: a specific marker of the germline in crassostrea gigas |
publisher |
Elsevier |
publishDate |
2004 |
url |
https://archimer.ifremer.fr/doc/2004/publication-646.pdf https://doi.org/10.1016/j.bbrc.2004.01.145 https://archimer.ifremer.fr/doc/00000/646/ |
long_lat |
ENVELOPE(25.177,25.177,67.587,67.587) |
geographic |
Vasa |
geographic_facet |
Vasa |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Biochemical and Biophysical Research Communications (0006-291X) (Elsevier), 2004-03 , Vol. 315 , N. 4 , P. 897-904 |
op_relation |
https://archimer.ifremer.fr/doc/2004/publication-646.pdf doi:10.1016/j.bbrc.2004.01.145 https://archimer.ifremer.fr/doc/00000/646/ |
op_rights |
2004 Elsevier Inc. All rights reserved info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.bbrc.2004.01.145 |
container_title |
Biochemical and Biophysical Research Communications |
container_volume |
315 |
container_issue |
4 |
container_start_page |
897 |
op_container_end_page |
904 |
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1766393503494963200 |