A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments
We present a new method to produce tetraploid Crassostrea gigas by cytochalasin B inhibition of polar body 2 expulsion in diploid females crossed with tetraploid males. This offers a means of direct introgression of genetic characters from selected diploid to tetraploid lines, avoiding a triploid st...
Published in: | Marine Biotechnology |
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2005
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Online Access: | https://archimer.ifremer.fr/doc/2005/publication-632.pdf https://doi.org/10.1007/s10126-004-0440-2 https://archimer.ifremer.fr/doc/00000/632/ |
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ftarchimer:oai:archimer.ifremer.fr:632 2023-05-15T15:57:32+02:00 A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments Mccombie, Helen Ledu, Christophe Phelipot, Pascal Lapegue, Sylvie Boudry, Pierre Gerard, Andre 2005-08 application/pdf https://archimer.ifremer.fr/doc/2005/publication-632.pdf https://doi.org/10.1007/s10126-004-0440-2 https://archimer.ifremer.fr/doc/00000/632/ eng eng Springer https://archimer.ifremer.fr/doc/2005/publication-632.pdf doi:10.1007/s10126-004-0440-2 https://archimer.ifremer.fr/doc/00000/632/ 2005 Publisher: Springer-Verlag New York info:eu-repo/semantics/openAccess restricted use Marine Biotechnology (1436-2228) (Springer), 2005-08 , Vol. 7 , N. 4 , P. 318-330 Oyster breeding Gynogenesis Polar body Cytochalasin B Polyploidy Crassostrea gigas text Publication info:eu-repo/semantics/article 2005 ftarchimer https://doi.org/10.1007/s10126-004-0440-2 2021-09-23T20:13:16Z We present a new method to produce tetraploid Crassostrea gigas by cytochalasin B inhibition of polar body 2 expulsion in diploid females crossed with tetraploid males. This offers a means of direct introgression of genetic characters from selected diploid to tetraploid lines, avoiding a triploid step. Offspring larval ploidy shifted over time and depended on size, with tetraploids more frequent among the smaller larvae and triploids among the large. Viable tetraploids were found at 4 and 6 months, indicating the technique was successful. The possibility that gynogenesis occurred was tested by microsatellite analysis to confirm the presence of paternally inherited alleles. These were present in all animals of the 2n x 4n + CB (female first) cross. However, a 4n x 2n + CB cross produced triploids, including some gynogens. Our method illustrates for the first time that diploid C. gigas eggs, if selected for large size, can give viable tetraploid offspring. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Marine Biotechnology 7 4 318 330 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Oyster breeding Gynogenesis Polar body Cytochalasin B Polyploidy Crassostrea gigas |
spellingShingle |
Oyster breeding Gynogenesis Polar body Cytochalasin B Polyploidy Crassostrea gigas Mccombie, Helen Ledu, Christophe Phelipot, Pascal Lapegue, Sylvie Boudry, Pierre Gerard, Andre A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments |
topic_facet |
Oyster breeding Gynogenesis Polar body Cytochalasin B Polyploidy Crassostrea gigas |
description |
We present a new method to produce tetraploid Crassostrea gigas by cytochalasin B inhibition of polar body 2 expulsion in diploid females crossed with tetraploid males. This offers a means of direct introgression of genetic characters from selected diploid to tetraploid lines, avoiding a triploid step. Offspring larval ploidy shifted over time and depended on size, with tetraploids more frequent among the smaller larvae and triploids among the large. Viable tetraploids were found at 4 and 6 months, indicating the technique was successful. The possibility that gynogenesis occurred was tested by microsatellite analysis to confirm the presence of paternally inherited alleles. These were present in all animals of the 2n x 4n + CB (female first) cross. However, a 4n x 2n + CB cross produced triploids, including some gynogens. Our method illustrates for the first time that diploid C. gigas eggs, if selected for large size, can give viable tetraploid offspring. |
format |
Article in Journal/Newspaper |
author |
Mccombie, Helen Ledu, Christophe Phelipot, Pascal Lapegue, Sylvie Boudry, Pierre Gerard, Andre |
author_facet |
Mccombie, Helen Ledu, Christophe Phelipot, Pascal Lapegue, Sylvie Boudry, Pierre Gerard, Andre |
author_sort |
Mccombie, Helen |
title |
A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments |
title_short |
A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments |
title_full |
A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments |
title_fullStr |
A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments |
title_full_unstemmed |
A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments |
title_sort |
complementary method for production of tetraploid crassostrea gigas using crosses between diploids and tetraploids with cytochalasin b treatments |
publisher |
Springer |
publishDate |
2005 |
url |
https://archimer.ifremer.fr/doc/2005/publication-632.pdf https://doi.org/10.1007/s10126-004-0440-2 https://archimer.ifremer.fr/doc/00000/632/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Marine Biotechnology (1436-2228) (Springer), 2005-08 , Vol. 7 , N. 4 , P. 318-330 |
op_relation |
https://archimer.ifremer.fr/doc/2005/publication-632.pdf doi:10.1007/s10126-004-0440-2 https://archimer.ifremer.fr/doc/00000/632/ |
op_rights |
2005 Publisher: Springer-Verlag New York info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1007/s10126-004-0440-2 |
container_title |
Marine Biotechnology |
container_volume |
7 |
container_issue |
4 |
container_start_page |
318 |
op_container_end_page |
330 |
_version_ |
1766393201272291328 |