Production of exopolysaccharides by Antarctic marine bacterial isolates
Aims: This study was undertaken to examine and characterize Antarctic marine bacterial isolates and the exopolysaccharides (EPS) they produce in laboratory culture. Methods and Results: Two EPS-producing bacterial strains CAM025 and CAM036 were isolated from particulate material sampled from seawate...
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ftarchimer:oai:archimer.ifremer.fr:629 2023-05-15T14:04:56+02:00 Production of exopolysaccharides by Antarctic marine bacterial isolates Nichols, C Garon, Sandrine Bowman, J Raguenes, Gerard Guezennec, Jean 2004-05 application/pdf https://archimer.ifremer.fr/doc/2004/publication-629.pdf https://doi.org/10.1111/j.1365-2672.2004.02216.x https://archimer.ifremer.fr/doc/00000/629/ eng eng Blackwell science https://archimer.ifremer.fr/doc/2004/publication-629.pdf doi:10.1111/j.1365-2672.2004.02216.x https://archimer.ifremer.fr/doc/00000/629/ 2004 Blackwell Publishing, Inc. info:eu-repo/semantics/openAccess restricted use Journal of Applied Microbiology (1364-5072) (Blackwell science), 2004-05 , Vol. 96 , N. 5 , P. 1057-1066 Sea ice Pseudoalteromonas spp Particulate organic material Exopolysaccharides Antarctic marine bacteria text Publication info:eu-repo/semantics/article 2004 ftarchimer https://doi.org/10.1111/j.1365-2672.2004.02216.x 2021-09-23T20:13:12Z Aims: This study was undertaken to examine and characterize Antarctic marine bacterial isolates and the exopolysaccharides (EPS) they produce in laboratory culture. Methods and Results: Two EPS-producing bacterial strains CAM025 and CAM036 were isolated from particulate material sampled from seawater and sea ice in the southern ocean. Analyses of 16S rDNA sequences placed these isolates in the genus Pseudoalteromonas. In batch culture, both strains produced EPS. The yield of EPS produced by CAM025 was 30-fold higher at -2 and 10degreesC than at 20degreesC. Crude chemical analyses showed that these EPS were composed primarily of neutral sugars and uronic acids with sulphates. Gas chromatographic analysis of monosaccharides confirmed these gross compositional findings and molar ratios of monosaccharides revealed differences between the two EPS. Conclusions: The EPS produced by Antarctic bacterial isolates examined in this study appeared to be polyanionic and, therefore, 'sticky' with respect to cations such as trace metals. Significance and Impact of the Study: As the availability of iron as a trace metal is of critical importance in the southern ocean where it is know to limit primary production, the role of these bacterial EPS in the Antarctic marine environment has important ecological implications. Article in Journal/Newspaper Antarc* Antarctic Sea ice Southern Ocean Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Antarctic Southern Ocean The Antarctic Journal of Applied Microbiology 96 5 1057 1066 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Sea ice Pseudoalteromonas spp Particulate organic material Exopolysaccharides Antarctic marine bacteria |
spellingShingle |
Sea ice Pseudoalteromonas spp Particulate organic material Exopolysaccharides Antarctic marine bacteria Nichols, C Garon, Sandrine Bowman, J Raguenes, Gerard Guezennec, Jean Production of exopolysaccharides by Antarctic marine bacterial isolates |
topic_facet |
Sea ice Pseudoalteromonas spp Particulate organic material Exopolysaccharides Antarctic marine bacteria |
description |
Aims: This study was undertaken to examine and characterize Antarctic marine bacterial isolates and the exopolysaccharides (EPS) they produce in laboratory culture. Methods and Results: Two EPS-producing bacterial strains CAM025 and CAM036 were isolated from particulate material sampled from seawater and sea ice in the southern ocean. Analyses of 16S rDNA sequences placed these isolates in the genus Pseudoalteromonas. In batch culture, both strains produced EPS. The yield of EPS produced by CAM025 was 30-fold higher at -2 and 10degreesC than at 20degreesC. Crude chemical analyses showed that these EPS were composed primarily of neutral sugars and uronic acids with sulphates. Gas chromatographic analysis of monosaccharides confirmed these gross compositional findings and molar ratios of monosaccharides revealed differences between the two EPS. Conclusions: The EPS produced by Antarctic bacterial isolates examined in this study appeared to be polyanionic and, therefore, 'sticky' with respect to cations such as trace metals. Significance and Impact of the Study: As the availability of iron as a trace metal is of critical importance in the southern ocean where it is know to limit primary production, the role of these bacterial EPS in the Antarctic marine environment has important ecological implications. |
format |
Article in Journal/Newspaper |
author |
Nichols, C Garon, Sandrine Bowman, J Raguenes, Gerard Guezennec, Jean |
author_facet |
Nichols, C Garon, Sandrine Bowman, J Raguenes, Gerard Guezennec, Jean |
author_sort |
Nichols, C |
title |
Production of exopolysaccharides by Antarctic marine bacterial isolates |
title_short |
Production of exopolysaccharides by Antarctic marine bacterial isolates |
title_full |
Production of exopolysaccharides by Antarctic marine bacterial isolates |
title_fullStr |
Production of exopolysaccharides by Antarctic marine bacterial isolates |
title_full_unstemmed |
Production of exopolysaccharides by Antarctic marine bacterial isolates |
title_sort |
production of exopolysaccharides by antarctic marine bacterial isolates |
publisher |
Blackwell science |
publishDate |
2004 |
url |
https://archimer.ifremer.fr/doc/2004/publication-629.pdf https://doi.org/10.1111/j.1365-2672.2004.02216.x https://archimer.ifremer.fr/doc/00000/629/ |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Sea ice Southern Ocean |
op_source |
Journal of Applied Microbiology (1364-5072) (Blackwell science), 2004-05 , Vol. 96 , N. 5 , P. 1057-1066 |
op_relation |
https://archimer.ifremer.fr/doc/2004/publication-629.pdf doi:10.1111/j.1365-2672.2004.02216.x https://archimer.ifremer.fr/doc/00000/629/ |
op_rights |
2004 Blackwell Publishing, Inc. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1111/j.1365-2672.2004.02216.x |
container_title |
Journal of Applied Microbiology |
container_volume |
96 |
container_issue |
5 |
container_start_page |
1057 |
op_container_end_page |
1066 |
_version_ |
1766276386679422976 |