Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells
Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae has been repeatedly isolated from diseased animals and the majority of the strains have been demonstrated to be viru...
Published in: | Environmental Microbiology |
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Online Access: | https://archimer.ifremer.fr/doc/00510/62129/66343.pdf https://doi.org/10.1111/1462-2920.14776 https://archimer.ifremer.fr/doc/00510/62129/ |
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ftarchimer:oai:archimer.ifremer.fr:62129 2023-05-15T15:58:37+02:00 Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells Piel, Damien Bruto, Maxime James, Adele Labreuche, Yannick Lambert, Christophe Janicot, Adrian Chenivesse, Sabine Petton, Bruno Wegner, K. Mathias Stoudmann, Candice Blokesch, Melanie Le Roux, Frederique 2020-10 application/pdf https://archimer.ifremer.fr/doc/00510/62129/66343.pdf https://doi.org/10.1111/1462-2920.14776 https://archimer.ifremer.fr/doc/00510/62129/ eng eng Wiley https://archimer.ifremer.fr/doc/00510/62129/66343.pdf doi:10.1111/1462-2920.14776 https://archimer.ifremer.fr/doc/00510/62129/ info:eu-repo/semantics/openAccess restricted use Environmental Microbiology (1462-2912) (Wiley), 2020-10 , Vol. 22 , N. 10 , P. 4198-4211 text Publication info:eu-repo/semantics/article 2020 ftarchimer https://doi.org/10.1111/1462-2920.14776 2021-09-23T20:33:23Z Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae has been repeatedly isolated from diseased animals and the majority of the strains have been demonstrated to be virulent for oysters. In this study we showed that oyster farms exhibited a high prevalence of a virulence plasmid carried by V. crassostreae while oysters, at an adult stage, were reservoirs of this virulent population. The pathogenicity of V. crassostreae depends on a novel transcriptional regulator, which activates the bidirectional promoter of a Type 6 Secretion System (T6SS) genes cluster. Both the T6SS and a second chromosomal virulence factor, r5.7, are necessary for virulence but act independently to cause to hemocyte (oyster immune cell) cytotoxicity. A phylogenetically closely related T6SS was identified in V. aestuarianus and V. tapetis, which infect adult oysters and clams, respectively. We propose that hemocyte cytotoxicity, is a lethality trait shared by a broad range of mollusk pathogens and we speculate that T6SS was involved in parallel evolution of pathogen for mollusks. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Environmental Microbiology 22 10 4198 4211 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
description |
Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae has been repeatedly isolated from diseased animals and the majority of the strains have been demonstrated to be virulent for oysters. In this study we showed that oyster farms exhibited a high prevalence of a virulence plasmid carried by V. crassostreae while oysters, at an adult stage, were reservoirs of this virulent population. The pathogenicity of V. crassostreae depends on a novel transcriptional regulator, which activates the bidirectional promoter of a Type 6 Secretion System (T6SS) genes cluster. Both the T6SS and a second chromosomal virulence factor, r5.7, are necessary for virulence but act independently to cause to hemocyte (oyster immune cell) cytotoxicity. A phylogenetically closely related T6SS was identified in V. aestuarianus and V. tapetis, which infect adult oysters and clams, respectively. We propose that hemocyte cytotoxicity, is a lethality trait shared by a broad range of mollusk pathogens and we speculate that T6SS was involved in parallel evolution of pathogen for mollusks. |
format |
Article in Journal/Newspaper |
author |
Piel, Damien Bruto, Maxime James, Adele Labreuche, Yannick Lambert, Christophe Janicot, Adrian Chenivesse, Sabine Petton, Bruno Wegner, K. Mathias Stoudmann, Candice Blokesch, Melanie Le Roux, Frederique |
spellingShingle |
Piel, Damien Bruto, Maxime James, Adele Labreuche, Yannick Lambert, Christophe Janicot, Adrian Chenivesse, Sabine Petton, Bruno Wegner, K. Mathias Stoudmann, Candice Blokesch, Melanie Le Roux, Frederique Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
author_facet |
Piel, Damien Bruto, Maxime James, Adele Labreuche, Yannick Lambert, Christophe Janicot, Adrian Chenivesse, Sabine Petton, Bruno Wegner, K. Mathias Stoudmann, Candice Blokesch, Melanie Le Roux, Frederique |
author_sort |
Piel, Damien |
title |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_short |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_full |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_fullStr |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_full_unstemmed |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_sort |
selection of vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
publisher |
Wiley |
publishDate |
2020 |
url |
https://archimer.ifremer.fr/doc/00510/62129/66343.pdf https://doi.org/10.1111/1462-2920.14776 https://archimer.ifremer.fr/doc/00510/62129/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Environmental Microbiology (1462-2912) (Wiley), 2020-10 , Vol. 22 , N. 10 , P. 4198-4211 |
op_relation |
https://archimer.ifremer.fr/doc/00510/62129/66343.pdf doi:10.1111/1462-2920.14776 https://archimer.ifremer.fr/doc/00510/62129/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1111/1462-2920.14776 |
container_title |
Environmental Microbiology |
container_volume |
22 |
container_issue |
10 |
container_start_page |
4198 |
op_container_end_page |
4211 |
_version_ |
1766394393775833088 |