Fine-scale temporal dynamics of herpes virus and vibrios in seawater during a polymicrobial infection in the Pacific oyster Crassostrea gigas
The Pacific oyster Crassostrea gigas is currently being impacted by a polymicrobial disease that involves early viral infection by ostreid herpesvirus-1 (OsHV-1) followed by a secondary bacterial infection leading to death. A widely used method of inducing infection consists of placing specific path...
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ftarchimer:oai:archimer.ifremer.fr:61870 2023-05-15T15:58:01+02:00 Fine-scale temporal dynamics of herpes virus and vibrios in seawater during a polymicrobial infection in the Pacific oyster Crassostrea gigas Petton, Bruno De Lorgeril, Julien Mitta, Guillaume Daigle, Gaétan Pernet, Fabrice Alunno Bruscia, Marianne 2019 application/pdf https://archimer.ifremer.fr/doc/00507/61870/65940.pdf https://archimer.ifremer.fr/doc/00507/61870/65941.pdf https://doi.org/10.3354/dao03384 https://archimer.ifremer.fr/doc/00507/61870/ eng eng Inter-Research https://archimer.ifremer.fr/doc/00507/61870/65940.pdf https://archimer.ifremer.fr/doc/00507/61870/65941.pdf doi:10.3354/dao03384 https://archimer.ifremer.fr/doc/00507/61870/ info:eu-repo/semantics/openAccess restricted use Diseases Of Aquatic Organisms (0177-5103) (Inter-Research), 2019 , Vol. 135 , N. 2 , P. 97-106 Aquaculture Bivalve Epidemiology Health Polymicrobial disease OSHV-1 Pacific oyster mortality syndrome POMS text Publication info:eu-repo/semantics/article 2019 ftarchimer https://doi.org/10.3354/dao03384 2021-09-23T20:33:14Z The Pacific oyster Crassostrea gigas is currently being impacted by a polymicrobial disease that involves early viral infection by ostreid herpesvirus-1 (OsHV-1) followed by a secondary bacterial infection leading to death. A widely used method of inducing infection consists of placing specific pathogen-free oysters (‘recipients’) in cohabitation in the laboratory with diseased oysters that were naturally infected in the field (‘donors’). With this method, we evaluated the temporal dynamics of pathogen release in seawater and the cohabitation time necessary for disease transmission and expression. We showed that OsHV-1 and Vibrio spp. in the seawater peaked concomitantly during the first 48 h and decreased thereafter. We found that 1.5 h of cohabitation with donors was enough time to transmit pathogens to recipients and to induce mortality later, reflecting the highly contagious nature of the disease. Finally, mortality of recipients was associated with increasing cohabitation time with donors until reaching a plateau at 20%. This reflects the cumulative effect of exposure to pathogens. The optimal cohabitation time was 5−6 d, the mortality of recipients occurring 1−2 d earlier. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Diseases of Aquatic Organisms 135 2 97 106 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Aquaculture Bivalve Epidemiology Health Polymicrobial disease OSHV-1 Pacific oyster mortality syndrome POMS |
spellingShingle |
Aquaculture Bivalve Epidemiology Health Polymicrobial disease OSHV-1 Pacific oyster mortality syndrome POMS Petton, Bruno De Lorgeril, Julien Mitta, Guillaume Daigle, Gaétan Pernet, Fabrice Alunno Bruscia, Marianne Fine-scale temporal dynamics of herpes virus and vibrios in seawater during a polymicrobial infection in the Pacific oyster Crassostrea gigas |
topic_facet |
Aquaculture Bivalve Epidemiology Health Polymicrobial disease OSHV-1 Pacific oyster mortality syndrome POMS |
description |
The Pacific oyster Crassostrea gigas is currently being impacted by a polymicrobial disease that involves early viral infection by ostreid herpesvirus-1 (OsHV-1) followed by a secondary bacterial infection leading to death. A widely used method of inducing infection consists of placing specific pathogen-free oysters (‘recipients’) in cohabitation in the laboratory with diseased oysters that were naturally infected in the field (‘donors’). With this method, we evaluated the temporal dynamics of pathogen release in seawater and the cohabitation time necessary for disease transmission and expression. We showed that OsHV-1 and Vibrio spp. in the seawater peaked concomitantly during the first 48 h and decreased thereafter. We found that 1.5 h of cohabitation with donors was enough time to transmit pathogens to recipients and to induce mortality later, reflecting the highly contagious nature of the disease. Finally, mortality of recipients was associated with increasing cohabitation time with donors until reaching a plateau at 20%. This reflects the cumulative effect of exposure to pathogens. The optimal cohabitation time was 5−6 d, the mortality of recipients occurring 1−2 d earlier. |
format |
Article in Journal/Newspaper |
author |
Petton, Bruno De Lorgeril, Julien Mitta, Guillaume Daigle, Gaétan Pernet, Fabrice Alunno Bruscia, Marianne |
author_facet |
Petton, Bruno De Lorgeril, Julien Mitta, Guillaume Daigle, Gaétan Pernet, Fabrice Alunno Bruscia, Marianne |
author_sort |
Petton, Bruno |
title |
Fine-scale temporal dynamics of herpes virus and vibrios in seawater during a polymicrobial infection in the Pacific oyster Crassostrea gigas |
title_short |
Fine-scale temporal dynamics of herpes virus and vibrios in seawater during a polymicrobial infection in the Pacific oyster Crassostrea gigas |
title_full |
Fine-scale temporal dynamics of herpes virus and vibrios in seawater during a polymicrobial infection in the Pacific oyster Crassostrea gigas |
title_fullStr |
Fine-scale temporal dynamics of herpes virus and vibrios in seawater during a polymicrobial infection in the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Fine-scale temporal dynamics of herpes virus and vibrios in seawater during a polymicrobial infection in the Pacific oyster Crassostrea gigas |
title_sort |
fine-scale temporal dynamics of herpes virus and vibrios in seawater during a polymicrobial infection in the pacific oyster crassostrea gigas |
publisher |
Inter-Research |
publishDate |
2019 |
url |
https://archimer.ifremer.fr/doc/00507/61870/65940.pdf https://archimer.ifremer.fr/doc/00507/61870/65941.pdf https://doi.org/10.3354/dao03384 https://archimer.ifremer.fr/doc/00507/61870/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Diseases Of Aquatic Organisms (0177-5103) (Inter-Research), 2019 , Vol. 135 , N. 2 , P. 97-106 |
op_relation |
https://archimer.ifremer.fr/doc/00507/61870/65940.pdf https://archimer.ifremer.fr/doc/00507/61870/65941.pdf doi:10.3354/dao03384 https://archimer.ifremer.fr/doc/00507/61870/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.3354/dao03384 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
135 |
container_issue |
2 |
container_start_page |
97 |
op_container_end_page |
106 |
_version_ |
1766393738307829760 |