Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)(4), (GT)(7) and (TA)(10) chromosome patterns
Chromosome identification is essential in oyster genomic research. Fluorescence in situ hybridization (FISH) offers new opportunities for the identification of oyster chromosomes. It has been used to locate satellite DNAs, telomeres or ribosomal DNA sequences. However, regarding chromosome identific...
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Indian Academy of Sciences
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ftarchimer:oai:archimer.ifremer.fr:6132 2023-05-15T15:57:37+02:00 Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)(4), (GT)(7) and (TA)(10) chromosome patterns Bouilly, Karine Chaves, R. Leitao, A. Benabdelmouna, Abdellah Guedes Pinto, H. 2008-08 application/pdf https://archimer.ifremer.fr/doc/2008/publication-6132.pdf https://archimer.ifremer.fr/doc/00000/6132/ eng eng Indian Academy of Sciences https://archimer.ifremer.fr/doc/2008/publication-6132.pdf https://archimer.ifremer.fr/doc/00000/6132/ 2008 Indian Academy of Sciences info:eu-repo/semantics/openAccess restricted use Journal of Genetics (0022-1333) (Indian Academy of Sciences), 2008-08 , Vol. 87 , N. 2 , P. 119-125 Simple sequence repeats (SSRs) Mollusks Fluorescence in situ hybridization (FISH) Crassostrea gigas Chromosome identification text Publication info:eu-repo/semantics/article 2008 ftarchimer 2021-09-23T20:17:04Z Chromosome identification is essential in oyster genomic research. Fluorescence in situ hybridization (FISH) offers new opportunities for the identification of oyster chromosomes. It has been used to locate satellite DNAs, telomeres or ribosomal DNA sequences. However, regarding chromosome identification, no study has been conducted with simple sequence repeats (SSRs). FISH was used to probe the physical organization of three particular SSRs, (GGAT)(4), (GT)(7) and (TA)(10) onto metaphase chromosomes of the Pacific oyster, Crassostrea gigas. Hybridization signals were observed in all the SSR probes, but the distribution and intensity of signals varied according to the oligonucleotide repeat. The intercalary, centromeric and telomeric bands were observed along the chromosomes, and for each particular repeat every chromosome pair presented a similar pattern, allowing karyotypic analysis with all the SSRs tested. Our study is the first in mollusks to show the application of SSR in situ hybridization for chromosome identification and karyotyping. This technique can be a useful tool for oyster comparative studies and to understand genome organization in different oyster taxa. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Simple sequence repeats (SSRs) Mollusks Fluorescence in situ hybridization (FISH) Crassostrea gigas Chromosome identification |
spellingShingle |
Simple sequence repeats (SSRs) Mollusks Fluorescence in situ hybridization (FISH) Crassostrea gigas Chromosome identification Bouilly, Karine Chaves, R. Leitao, A. Benabdelmouna, Abdellah Guedes Pinto, H. Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)(4), (GT)(7) and (TA)(10) chromosome patterns |
topic_facet |
Simple sequence repeats (SSRs) Mollusks Fluorescence in situ hybridization (FISH) Crassostrea gigas Chromosome identification |
description |
Chromosome identification is essential in oyster genomic research. Fluorescence in situ hybridization (FISH) offers new opportunities for the identification of oyster chromosomes. It has been used to locate satellite DNAs, telomeres or ribosomal DNA sequences. However, regarding chromosome identification, no study has been conducted with simple sequence repeats (SSRs). FISH was used to probe the physical organization of three particular SSRs, (GGAT)(4), (GT)(7) and (TA)(10) onto metaphase chromosomes of the Pacific oyster, Crassostrea gigas. Hybridization signals were observed in all the SSR probes, but the distribution and intensity of signals varied according to the oligonucleotide repeat. The intercalary, centromeric and telomeric bands were observed along the chromosomes, and for each particular repeat every chromosome pair presented a similar pattern, allowing karyotypic analysis with all the SSRs tested. Our study is the first in mollusks to show the application of SSR in situ hybridization for chromosome identification and karyotyping. This technique can be a useful tool for oyster comparative studies and to understand genome organization in different oyster taxa. |
format |
Article in Journal/Newspaper |
author |
Bouilly, Karine Chaves, R. Leitao, A. Benabdelmouna, Abdellah Guedes Pinto, H. |
author_facet |
Bouilly, Karine Chaves, R. Leitao, A. Benabdelmouna, Abdellah Guedes Pinto, H. |
author_sort |
Bouilly, Karine |
title |
Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)(4), (GT)(7) and (TA)(10) chromosome patterns |
title_short |
Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)(4), (GT)(7) and (TA)(10) chromosome patterns |
title_full |
Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)(4), (GT)(7) and (TA)(10) chromosome patterns |
title_fullStr |
Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)(4), (GT)(7) and (TA)(10) chromosome patterns |
title_full_unstemmed |
Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)(4), (GT)(7) and (TA)(10) chromosome patterns |
title_sort |
chromosomal organization of simple sequence repeats in the pacific oyster (crassostrea gigas): (ggat)(4), (gt)(7) and (ta)(10) chromosome patterns |
publisher |
Indian Academy of Sciences |
publishDate |
2008 |
url |
https://archimer.ifremer.fr/doc/2008/publication-6132.pdf https://archimer.ifremer.fr/doc/00000/6132/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Journal of Genetics (0022-1333) (Indian Academy of Sciences), 2008-08 , Vol. 87 , N. 2 , P. 119-125 |
op_relation |
https://archimer.ifremer.fr/doc/2008/publication-6132.pdf https://archimer.ifremer.fr/doc/00000/6132/ |
op_rights |
2008 Indian Academy of Sciences info:eu-repo/semantics/openAccess restricted use |
_version_ |
1766393282354479104 |