Prevalence, Molecular Identification, and Risk Factors for Cryptosporidium Infection in Edible Marine Fish: A Survey Across Sea Areas Surrounding France
Cryptosporidium, a zoonotic pathogen, is able to infect a wide range of hosts including wild and domestic animals, and humans. Although it is well known that some parasites are both fish pathogens and recognized agents of zoonosis with a public health impact, little information is available concerni...
Published in: | Frontiers in Microbiology |
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ftarchimer:oai:archimer.ifremer.fr:60864 2023-05-15T17:38:44+02:00 Prevalence, Molecular Identification, and Risk Factors for Cryptosporidium Infection in Edible Marine Fish: A Survey Across Sea Areas Surrounding France Certad, Gabriela Follet, Jérôme Gantois, Nausicaa Hammouma-ghelboun, Ourida Guyot, Karine Benamrouz-vanneste, Sadia Fréalle, Emilie Seesao, Yuwalee Delaire, Baptiste Creusy, Colette Even, Gaël Verrez-bagnis, Veronique Ryan, Una Gay, Mélanie Aliouat-denis, Cécile Viscogliosi, Eric 2019-05 application/pdf https://archimer.ifremer.fr/doc/00497/60864/64198.pdf https://archimer.ifremer.fr/doc/00497/60864/64199.tiff https://archimer.ifremer.fr/doc/00497/60864/64200.tiff https://doi.org/10.3389/fmicb.2019.01037 https://archimer.ifremer.fr/doc/00497/60864/ eng eng Frontiers Media SA https://archimer.ifremer.fr/doc/00497/60864/64198.pdf https://archimer.ifremer.fr/doc/00497/60864/64199.tiff https://archimer.ifremer.fr/doc/00497/60864/64200.tiff doi:10.3389/fmicb.2019.01037 https://archimer.ifremer.fr/doc/00497/60864/ info:eu-repo/semantics/openAccess restricted use Frontiers In Microbiology (1664-302X) (Frontiers Media SA), 2019-05 , Vol. 10 , N. 1037 , P. 15p. Cryptosporidium edible marine fish 18S rRNA gene gp60 molecular epidemiology phylogeny novel genotypes European seas text Publication info:eu-repo/semantics/article 2019 ftarchimer https://doi.org/10.3389/fmicb.2019.01037 2021-09-23T20:32:46Z Cryptosporidium, a zoonotic pathogen, is able to infect a wide range of hosts including wild and domestic animals, and humans. Although it is well known that some parasites are both fish pathogens and recognized agents of zoonosis with a public health impact, little information is available concerning the prevalence of Cryptosporidium in wild aquatic environments. To evaluate the prevalence of Cryptosporidium spp. in commercially important edible marine fish in different European seas (English channel, North sea, Bay of Biscay, Celtic sea and Mediterranean sea), 1,853 specimens were collected as part of two surveys. Nested PCR followed by sequence analysis at the 18S rRNA gene locus was used to identify Cryptosporidium spp. The overall prevalence of Cryptosporidium spp. in sampled fish reached 2.3% (35 out of 1,508) in a first campaign and 3.2% (11 out of 345) in a second campaign. Sequence and phylogenetic analysis of positive samples identified Cryptosporidium parvum (n = 10) and seven genotypes which exhibited between 7.3 and 10.1% genetic distance from C. molnari, with the exception of one genotype which exhibited only 0.5–0.7% genetic distance from C. molnari. Among 31 analyzed fish species, 11 (35.5%) were identified as potential hosts for Cryptosporidium. A higher prevalence of Cryptosporidium spp. was observed in larger fish, in fish collected during the spring-summer period, and in those caught in the North East Atlantic. Pollachius virens (saithe) was the most frequently Cryptosporidium positive species. In fish infected by other parasites, the risk of being Cryptosporidium positive increased 10-fold (OR: 9.95, CI: 2.32–40.01.04, P = 0.0002). Four gp60 subtypes were detected among the C. parvum positive samples: IIaA13G1R1, IIaA15G2R1, IIaA17G2R1, and IIaA18G3R1. These C. parvum subtypes have been previously detected in terrestrial mammals and may constitute an additional source of infection for other animals and in particular for humans. Microscopical examination of histological sections confirmed the presence of round bodies suggestive of the development of C. parvum within digestive glands. We report herein the first epidemiological and molecular data concerning the detection of Cryptosporidium in edible marine fish in European seas surrounding France broadening its host range and uncovering potential novel infection routes. Article in Journal/Newspaper North East Atlantic Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Frontiers in Microbiology 10 |
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Open Polar |
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Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Cryptosporidium edible marine fish 18S rRNA gene gp60 molecular epidemiology phylogeny novel genotypes European seas |
spellingShingle |
Cryptosporidium edible marine fish 18S rRNA gene gp60 molecular epidemiology phylogeny novel genotypes European seas Certad, Gabriela Follet, Jérôme Gantois, Nausicaa Hammouma-ghelboun, Ourida Guyot, Karine Benamrouz-vanneste, Sadia Fréalle, Emilie Seesao, Yuwalee Delaire, Baptiste Creusy, Colette Even, Gaël Verrez-bagnis, Veronique Ryan, Una Gay, Mélanie Aliouat-denis, Cécile Viscogliosi, Eric Prevalence, Molecular Identification, and Risk Factors for Cryptosporidium Infection in Edible Marine Fish: A Survey Across Sea Areas Surrounding France |
topic_facet |
Cryptosporidium edible marine fish 18S rRNA gene gp60 molecular epidemiology phylogeny novel genotypes European seas |
description |
Cryptosporidium, a zoonotic pathogen, is able to infect a wide range of hosts including wild and domestic animals, and humans. Although it is well known that some parasites are both fish pathogens and recognized agents of zoonosis with a public health impact, little information is available concerning the prevalence of Cryptosporidium in wild aquatic environments. To evaluate the prevalence of Cryptosporidium spp. in commercially important edible marine fish in different European seas (English channel, North sea, Bay of Biscay, Celtic sea and Mediterranean sea), 1,853 specimens were collected as part of two surveys. Nested PCR followed by sequence analysis at the 18S rRNA gene locus was used to identify Cryptosporidium spp. The overall prevalence of Cryptosporidium spp. in sampled fish reached 2.3% (35 out of 1,508) in a first campaign and 3.2% (11 out of 345) in a second campaign. Sequence and phylogenetic analysis of positive samples identified Cryptosporidium parvum (n = 10) and seven genotypes which exhibited between 7.3 and 10.1% genetic distance from C. molnari, with the exception of one genotype which exhibited only 0.5–0.7% genetic distance from C. molnari. Among 31 analyzed fish species, 11 (35.5%) were identified as potential hosts for Cryptosporidium. A higher prevalence of Cryptosporidium spp. was observed in larger fish, in fish collected during the spring-summer period, and in those caught in the North East Atlantic. Pollachius virens (saithe) was the most frequently Cryptosporidium positive species. In fish infected by other parasites, the risk of being Cryptosporidium positive increased 10-fold (OR: 9.95, CI: 2.32–40.01.04, P = 0.0002). Four gp60 subtypes were detected among the C. parvum positive samples: IIaA13G1R1, IIaA15G2R1, IIaA17G2R1, and IIaA18G3R1. These C. parvum subtypes have been previously detected in terrestrial mammals and may constitute an additional source of infection for other animals and in particular for humans. Microscopical examination of histological sections confirmed the presence of round bodies suggestive of the development of C. parvum within digestive glands. We report herein the first epidemiological and molecular data concerning the detection of Cryptosporidium in edible marine fish in European seas surrounding France broadening its host range and uncovering potential novel infection routes. |
format |
Article in Journal/Newspaper |
author |
Certad, Gabriela Follet, Jérôme Gantois, Nausicaa Hammouma-ghelboun, Ourida Guyot, Karine Benamrouz-vanneste, Sadia Fréalle, Emilie Seesao, Yuwalee Delaire, Baptiste Creusy, Colette Even, Gaël Verrez-bagnis, Veronique Ryan, Una Gay, Mélanie Aliouat-denis, Cécile Viscogliosi, Eric |
author_facet |
Certad, Gabriela Follet, Jérôme Gantois, Nausicaa Hammouma-ghelboun, Ourida Guyot, Karine Benamrouz-vanneste, Sadia Fréalle, Emilie Seesao, Yuwalee Delaire, Baptiste Creusy, Colette Even, Gaël Verrez-bagnis, Veronique Ryan, Una Gay, Mélanie Aliouat-denis, Cécile Viscogliosi, Eric |
author_sort |
Certad, Gabriela |
title |
Prevalence, Molecular Identification, and Risk Factors for Cryptosporidium Infection in Edible Marine Fish: A Survey Across Sea Areas Surrounding France |
title_short |
Prevalence, Molecular Identification, and Risk Factors for Cryptosporidium Infection in Edible Marine Fish: A Survey Across Sea Areas Surrounding France |
title_full |
Prevalence, Molecular Identification, and Risk Factors for Cryptosporidium Infection in Edible Marine Fish: A Survey Across Sea Areas Surrounding France |
title_fullStr |
Prevalence, Molecular Identification, and Risk Factors for Cryptosporidium Infection in Edible Marine Fish: A Survey Across Sea Areas Surrounding France |
title_full_unstemmed |
Prevalence, Molecular Identification, and Risk Factors for Cryptosporidium Infection in Edible Marine Fish: A Survey Across Sea Areas Surrounding France |
title_sort |
prevalence, molecular identification, and risk factors for cryptosporidium infection in edible marine fish: a survey across sea areas surrounding france |
publisher |
Frontiers Media SA |
publishDate |
2019 |
url |
https://archimer.ifremer.fr/doc/00497/60864/64198.pdf https://archimer.ifremer.fr/doc/00497/60864/64199.tiff https://archimer.ifremer.fr/doc/00497/60864/64200.tiff https://doi.org/10.3389/fmicb.2019.01037 https://archimer.ifremer.fr/doc/00497/60864/ |
genre |
North East Atlantic |
genre_facet |
North East Atlantic |
op_source |
Frontiers In Microbiology (1664-302X) (Frontiers Media SA), 2019-05 , Vol. 10 , N. 1037 , P. 15p. |
op_relation |
https://archimer.ifremer.fr/doc/00497/60864/64198.pdf https://archimer.ifremer.fr/doc/00497/60864/64199.tiff https://archimer.ifremer.fr/doc/00497/60864/64200.tiff doi:10.3389/fmicb.2019.01037 https://archimer.ifremer.fr/doc/00497/60864/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.3389/fmicb.2019.01037 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
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1766139306960748544 |