A study of autophagy in hemocytes of the Pacific oyster, Crassostrea gigas
Macroautophagy is a mechanism that is involved in various cellular processes, including cellular homeostasis and innate immunity. This pathway has been described in organisms ranging in complexity from yeasts to mammals, and recent results indicate that it occurs in the mantle of the Pacific oyster,...
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Online Access: | https://archimer.ifremer.fr/doc/00487/59905/63087.pdf https://doi.org/10.1080/15548627.2019.1596490 https://archimer.ifremer.fr/doc/00487/59905/ |
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ftarchimer:oai:archimer.ifremer.fr:59905 2023-05-15T15:57:46+02:00 A study of autophagy in hemocytes of the Pacific oyster, Crassostrea gigas Picot, Sandy Morga, Benjamin Faury, Nicole Chollet, Bruno Dégremont, Lionel Travers, Marie-agnes Renault, Tristan Arzul, Isabelle 2019-10 application/pdf https://archimer.ifremer.fr/doc/00487/59905/63087.pdf https://doi.org/10.1080/15548627.2019.1596490 https://archimer.ifremer.fr/doc/00487/59905/ eng eng Informa UK Limited info:eu-repo/grantAgreement/EC/FP7/678589/EU//VIVALDI https://archimer.ifremer.fr/doc/00487/59905/63087.pdf doi:10.1080/15548627.2019.1596490 https://archimer.ifremer.fr/doc/00487/59905/ info:eu-repo/semantics/openAccess restricted use Autophagy (1554-8627) (Informa UK Limited), 2019-10 , Vol. 15 , N. 10 , P. 1801-1809 Autophagosome autophagy Crassostrea gigas flow cytometry fluorescence microscopy hemocytes transmission electron microscopy text Publication info:eu-repo/semantics/article 2019 ftarchimer https://doi.org/10.1080/15548627.2019.1596490 2021-09-23T20:32:23Z Macroautophagy is a mechanism that is involved in various cellular processes, including cellular homeostasis and innate immunity. This pathway has been described in organisms ranging in complexity from yeasts to mammals, and recent results indicate that it occurs in the mantle of the Pacific oyster, Crassostrea gigas. However, the autophagy pathway has never been explored in the hemocytes of C. gigas, which are the main effectors of its immune system and thus play a key role in the defence of the Pacific oyster against pathogens. To investigate autophagy in oyster hemocytes, tools currently used to monitor this mechanism in mammals, including flow cytometry, fluorescent microscopy and transmission electron microscopy, were adapted and applied to the hemocytes of the Pacific oyster. Oysters were exposed for 24 and 48 h to either an autophagy inducer (carbamazepine, which increases the production of autophagosomes) or an autophagy inhibitor (ammonium chloride, which prevents the degradation of autophagosomes). Autophagy was monitored in fresh hemocytes withdrawn from the adductor muscles of oysters using a combination of the three aforementioned methods. We successfully labelled autophagosomes and observed them by flow cytometry and fluorescence microscopy, and then used electron microscopy to observe ultrastructural modifications related to autophagy, including the presence of double-membrane-bound vacuoles. Our results demonstrated that autophagy occurs in hemocytes of C. gigas and can be modulated by molecules known to modulate autophagy in other organisms. This study describes an integrated approach that can be applied to investigate autophagy in marine bivalves at the cellular level. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Autophagy 15 10 1801 1809 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Autophagosome autophagy Crassostrea gigas flow cytometry fluorescence microscopy hemocytes transmission electron microscopy |
spellingShingle |
Autophagosome autophagy Crassostrea gigas flow cytometry fluorescence microscopy hemocytes transmission electron microscopy Picot, Sandy Morga, Benjamin Faury, Nicole Chollet, Bruno Dégremont, Lionel Travers, Marie-agnes Renault, Tristan Arzul, Isabelle A study of autophagy in hemocytes of the Pacific oyster, Crassostrea gigas |
topic_facet |
Autophagosome autophagy Crassostrea gigas flow cytometry fluorescence microscopy hemocytes transmission electron microscopy |
description |
Macroautophagy is a mechanism that is involved in various cellular processes, including cellular homeostasis and innate immunity. This pathway has been described in organisms ranging in complexity from yeasts to mammals, and recent results indicate that it occurs in the mantle of the Pacific oyster, Crassostrea gigas. However, the autophagy pathway has never been explored in the hemocytes of C. gigas, which are the main effectors of its immune system and thus play a key role in the defence of the Pacific oyster against pathogens. To investigate autophagy in oyster hemocytes, tools currently used to monitor this mechanism in mammals, including flow cytometry, fluorescent microscopy and transmission electron microscopy, were adapted and applied to the hemocytes of the Pacific oyster. Oysters were exposed for 24 and 48 h to either an autophagy inducer (carbamazepine, which increases the production of autophagosomes) or an autophagy inhibitor (ammonium chloride, which prevents the degradation of autophagosomes). Autophagy was monitored in fresh hemocytes withdrawn from the adductor muscles of oysters using a combination of the three aforementioned methods. We successfully labelled autophagosomes and observed them by flow cytometry and fluorescence microscopy, and then used electron microscopy to observe ultrastructural modifications related to autophagy, including the presence of double-membrane-bound vacuoles. Our results demonstrated that autophagy occurs in hemocytes of C. gigas and can be modulated by molecules known to modulate autophagy in other organisms. This study describes an integrated approach that can be applied to investigate autophagy in marine bivalves at the cellular level. |
format |
Article in Journal/Newspaper |
author |
Picot, Sandy Morga, Benjamin Faury, Nicole Chollet, Bruno Dégremont, Lionel Travers, Marie-agnes Renault, Tristan Arzul, Isabelle |
author_facet |
Picot, Sandy Morga, Benjamin Faury, Nicole Chollet, Bruno Dégremont, Lionel Travers, Marie-agnes Renault, Tristan Arzul, Isabelle |
author_sort |
Picot, Sandy |
title |
A study of autophagy in hemocytes of the Pacific oyster, Crassostrea gigas |
title_short |
A study of autophagy in hemocytes of the Pacific oyster, Crassostrea gigas |
title_full |
A study of autophagy in hemocytes of the Pacific oyster, Crassostrea gigas |
title_fullStr |
A study of autophagy in hemocytes of the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
A study of autophagy in hemocytes of the Pacific oyster, Crassostrea gigas |
title_sort |
study of autophagy in hemocytes of the pacific oyster, crassostrea gigas |
publisher |
Informa UK Limited |
publishDate |
2019 |
url |
https://archimer.ifremer.fr/doc/00487/59905/63087.pdf https://doi.org/10.1080/15548627.2019.1596490 https://archimer.ifremer.fr/doc/00487/59905/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Autophagy (1554-8627) (Informa UK Limited), 2019-10 , Vol. 15 , N. 10 , P. 1801-1809 |
op_relation |
info:eu-repo/grantAgreement/EC/FP7/678589/EU//VIVALDI https://archimer.ifremer.fr/doc/00487/59905/63087.pdf doi:10.1080/15548627.2019.1596490 https://archimer.ifremer.fr/doc/00487/59905/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1080/15548627.2019.1596490 |
container_title |
Autophagy |
container_volume |
15 |
container_issue |
10 |
container_start_page |
1801 |
op_container_end_page |
1809 |
_version_ |
1766393469388980224 |