In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement
Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ....
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ftarchimer:oai:archimer.ifremer.fr:58677 2023-05-15T15:57:39+02:00 In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement Foulon, Valentin Boudry, Pierre Artigaud, Sebastien Guérard, Fabienne Hellio, Claire 2019-01 application/pdf https://archimer.ifremer.fr/doc/00475/58677/61172.pdf https://doi.org/10.3390/ijms20010197 https://archimer.ifremer.fr/doc/00475/58677/ eng eng MDPI AG https://archimer.ifremer.fr/doc/00475/58677/61172.pdf doi:10.3390/ijms20010197 https://archimer.ifremer.fr/doc/00475/58677/ info:eu-repo/semantics/openAccess restricted use International Journal Of Molecular Sciences (1422-0067) (MDPI AG), 2019-01 , Vol. 20 , N. 1 , P. 197 (16p.) Crassostrea gigas Pacific oyster pediveliger larvae bioadhesive transcriptome text Publication info:eu-repo/semantics/article 2019 ftarchimer https://doi.org/10.3390/ijms20010197 2021-09-23T20:31:56Z Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the pediveliger stage. Our analysis revealed 59 sequences potentially implicated in adhesion of C. gigas larvae. Some related proteins contain conserved domains already described in other bioadhesives. We propose a hypothetic composition of C. gigas bioadhesive in which the protein constituent is probably composed of collagen and the von Willebrand Factor domain could play a role in adhesive cohesion. Genes coding for enzymes implicated in DOPA chemistry were also detected, indicating that this modification is also potentially present in the adhesive of pediveliger larvae. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific International Journal of Molecular Sciences 20 1 197 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Crassostrea gigas Pacific oyster pediveliger larvae bioadhesive transcriptome |
spellingShingle |
Crassostrea gigas Pacific oyster pediveliger larvae bioadhesive transcriptome Foulon, Valentin Boudry, Pierre Artigaud, Sebastien Guérard, Fabienne Hellio, Claire In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
topic_facet |
Crassostrea gigas Pacific oyster pediveliger larvae bioadhesive transcriptome |
description |
Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the pediveliger stage. Our analysis revealed 59 sequences potentially implicated in adhesion of C. gigas larvae. Some related proteins contain conserved domains already described in other bioadhesives. We propose a hypothetic composition of C. gigas bioadhesive in which the protein constituent is probably composed of collagen and the von Willebrand Factor domain could play a role in adhesive cohesion. Genes coding for enzymes implicated in DOPA chemistry were also detected, indicating that this modification is also potentially present in the adhesive of pediveliger larvae. |
format |
Article in Journal/Newspaper |
author |
Foulon, Valentin Boudry, Pierre Artigaud, Sebastien Guérard, Fabienne Hellio, Claire |
author_facet |
Foulon, Valentin Boudry, Pierre Artigaud, Sebastien Guérard, Fabienne Hellio, Claire |
author_sort |
Foulon, Valentin |
title |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_short |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_full |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_fullStr |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_full_unstemmed |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_sort |
in silico analysis of pacific oyster (crassostrea gigas) transcriptome over developmental stages reveals candidate genes for larval settlement |
publisher |
MDPI AG |
publishDate |
2019 |
url |
https://archimer.ifremer.fr/doc/00475/58677/61172.pdf https://doi.org/10.3390/ijms20010197 https://archimer.ifremer.fr/doc/00475/58677/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
International Journal Of Molecular Sciences (1422-0067) (MDPI AG), 2019-01 , Vol. 20 , N. 1 , P. 197 (16p.) |
op_relation |
https://archimer.ifremer.fr/doc/00475/58677/61172.pdf doi:10.3390/ijms20010197 https://archimer.ifremer.fr/doc/00475/58677/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.3390/ijms20010197 |
container_title |
International Journal of Molecular Sciences |
container_volume |
20 |
container_issue |
1 |
container_start_page |
197 |
_version_ |
1766393331809517568 |