Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata ) based on morphological and molecular analyses
We restudied the morphological complex comprising the cyst-based species Spiniferites elongatus/Spiniferites frigidus/Rottnestia amphicavata. We reviewed existing studies, and acquired new morphometric measurements of recent cysts from across the Northern Hemisphere, scanning electron microscopy (SE...
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Taylor & Francis Inc
2018
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Online Access: | https://archimer.ifremer.fr/doc/00471/58305/60846.pdf https://doi.org/10.1080/01916122.2018.1465736 https://archimer.ifremer.fr/doc/00471/58305/ |
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ftarchimer:oai:archimer.ifremer.fr:58305 2023-05-15T15:39:09+02:00 Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata ) based on morphological and molecular analyses Van Nieuwenhove, Nicolas Potvin, Eric Heikkila, Maija Pospelova, Vera Mertens, Kenneth Masure, Edwige Kucharska, Malgorzata Yang, Eun Jin Chomerat, Nicolas Zajaczkowski, Marek 2018-12 application/pdf https://archimer.ifremer.fr/doc/00471/58305/60846.pdf https://doi.org/10.1080/01916122.2018.1465736 https://archimer.ifremer.fr/doc/00471/58305/ eng eng Taylor & Francis Inc https://archimer.ifremer.fr/doc/00471/58305/60846.pdf doi:10.1080/01916122.2018.1465736 https://archimer.ifremer.fr/doc/00471/58305/ info:eu-repo/semantics/openAccess restricted use Palynology (0191-6122) (Taylor & Francis Inc), 2018-12 , Vol. 42 , N. Supl.1 , P. 111-134 Single-cell PCR Spiniferites ellipsoideus Spiniferites frigidus Rottnestia amphicavata morphologic variability taxonomy text Publication info:eu-repo/semantics/article 2018 ftarchimer https://doi.org/10.1080/01916122.2018.1465736 2021-09-23T20:31:56Z We restudied the morphological complex comprising the cyst-based species Spiniferites elongatus/Spiniferites frigidus/Rottnestia amphicavata. We reviewed existing studies, and acquired new morphometric measurements of recent cysts from across the Northern Hemisphere, scanning electron microscopy (SEM) observations of cysts from Barents Sea surface sediments, and genetic analyses of cysts from the Beaufort Sea. The measurements suggest that populations and morphospecies cannot be distinguished based on morphometric criteria. Furthermore, sequential sediment trap samples from Hudson Bay reveal that morphological variation can occur at the same location over a few weeks, arguing against a uniform morphological response to environmental parameters. The SEM observations reveal a consistent gonyaulacacean tabulation (Po, 4′, 6′′, 6c, 5 s, *6′′′, 1p, 1′′′′). The small and large subunit ribosomal RNA genes and the internal transcribed spacer sequences obtained from differing cysts from the Beaufort Sea with morphologies attributable to the complex, including forms that correspond to Rottnestia amphicavata, were all identical and conform to those of Gonyaulax elongata from the Orkney Islands. The molecular analyses thus support the conclusion that the morphological variability is not reflected genetically and occurs within one species. Based on arguments against the generic attribution of amphicavata to Rottnestia, the continuum between the extreme ends of the morphological range, and the molecular data, we suggest Rottnestia amphicavata to be conspecific with Spiniferites frigidus, and both to be junior synonyms of Spiniferites elongatus. The morphometric data further indicate that Spiniferites ellipsoideus, an elongate cyst from the Miocene, can also be considered a junior synonym of Spiniferites elongatus. It is recommended to use two informal types in census work (i.e. Spiniferites elongatus – Beaufort morphotype for morphologies formerly assignable to Spiniferites frigidus/Rottnestia amphicavata, and Spiniferites elongatus – Norwegian morphotype for cysts with strongly reduced processes) to separate specimens at both extreme ends of the morphological spectrum from typical specimens of Spiniferites elongatus. Article in Journal/Newspaper Barents Sea Beaufort Sea Hudson Bay Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Barents Sea Hudson Hudson Bay Palynology 42 sup1 111 134 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Single-cell PCR Spiniferites ellipsoideus Spiniferites frigidus Rottnestia amphicavata morphologic variability taxonomy |
spellingShingle |
Single-cell PCR Spiniferites ellipsoideus Spiniferites frigidus Rottnestia amphicavata morphologic variability taxonomy Van Nieuwenhove, Nicolas Potvin, Eric Heikkila, Maija Pospelova, Vera Mertens, Kenneth Masure, Edwige Kucharska, Malgorzata Yang, Eun Jin Chomerat, Nicolas Zajaczkowski, Marek Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata ) based on morphological and molecular analyses |
topic_facet |
Single-cell PCR Spiniferites ellipsoideus Spiniferites frigidus Rottnestia amphicavata morphologic variability taxonomy |
description |
We restudied the morphological complex comprising the cyst-based species Spiniferites elongatus/Spiniferites frigidus/Rottnestia amphicavata. We reviewed existing studies, and acquired new morphometric measurements of recent cysts from across the Northern Hemisphere, scanning electron microscopy (SEM) observations of cysts from Barents Sea surface sediments, and genetic analyses of cysts from the Beaufort Sea. The measurements suggest that populations and morphospecies cannot be distinguished based on morphometric criteria. Furthermore, sequential sediment trap samples from Hudson Bay reveal that morphological variation can occur at the same location over a few weeks, arguing against a uniform morphological response to environmental parameters. The SEM observations reveal a consistent gonyaulacacean tabulation (Po, 4′, 6′′, 6c, 5 s, *6′′′, 1p, 1′′′′). The small and large subunit ribosomal RNA genes and the internal transcribed spacer sequences obtained from differing cysts from the Beaufort Sea with morphologies attributable to the complex, including forms that correspond to Rottnestia amphicavata, were all identical and conform to those of Gonyaulax elongata from the Orkney Islands. The molecular analyses thus support the conclusion that the morphological variability is not reflected genetically and occurs within one species. Based on arguments against the generic attribution of amphicavata to Rottnestia, the continuum between the extreme ends of the morphological range, and the molecular data, we suggest Rottnestia amphicavata to be conspecific with Spiniferites frigidus, and both to be junior synonyms of Spiniferites elongatus. The morphometric data further indicate that Spiniferites ellipsoideus, an elongate cyst from the Miocene, can also be considered a junior synonym of Spiniferites elongatus. It is recommended to use two informal types in census work (i.e. Spiniferites elongatus – Beaufort morphotype for morphologies formerly assignable to Spiniferites frigidus/Rottnestia amphicavata, and Spiniferites elongatus – Norwegian morphotype for cysts with strongly reduced processes) to separate specimens at both extreme ends of the morphological spectrum from typical specimens of Spiniferites elongatus. |
format |
Article in Journal/Newspaper |
author |
Van Nieuwenhove, Nicolas Potvin, Eric Heikkila, Maija Pospelova, Vera Mertens, Kenneth Masure, Edwige Kucharska, Malgorzata Yang, Eun Jin Chomerat, Nicolas Zajaczkowski, Marek |
author_facet |
Van Nieuwenhove, Nicolas Potvin, Eric Heikkila, Maija Pospelova, Vera Mertens, Kenneth Masure, Edwige Kucharska, Malgorzata Yang, Eun Jin Chomerat, Nicolas Zajaczkowski, Marek |
author_sort |
Van Nieuwenhove, Nicolas |
title |
Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata ) based on morphological and molecular analyses |
title_short |
Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata ) based on morphological and molecular analyses |
title_full |
Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata ) based on morphological and molecular analyses |
title_fullStr |
Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata ) based on morphological and molecular analyses |
title_full_unstemmed |
Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata ) based on morphological and molecular analyses |
title_sort |
taxonomic revision of spiniferites elongatus (the resting stage of gonyaulax elongata ) based on morphological and molecular analyses |
publisher |
Taylor & Francis Inc |
publishDate |
2018 |
url |
https://archimer.ifremer.fr/doc/00471/58305/60846.pdf https://doi.org/10.1080/01916122.2018.1465736 https://archimer.ifremer.fr/doc/00471/58305/ |
geographic |
Barents Sea Hudson Hudson Bay |
geographic_facet |
Barents Sea Hudson Hudson Bay |
genre |
Barents Sea Beaufort Sea Hudson Bay |
genre_facet |
Barents Sea Beaufort Sea Hudson Bay |
op_source |
Palynology (0191-6122) (Taylor & Francis Inc), 2018-12 , Vol. 42 , N. Supl.1 , P. 111-134 |
op_relation |
https://archimer.ifremer.fr/doc/00471/58305/60846.pdf doi:10.1080/01916122.2018.1465736 https://archimer.ifremer.fr/doc/00471/58305/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1080/01916122.2018.1465736 |
container_title |
Palynology |
container_volume |
42 |
container_issue |
sup1 |
container_start_page |
111 |
op_container_end_page |
134 |
_version_ |
1766370618765213696 |