Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation
Parasites are key drivers of phytoplankton bloom dynamics and related aquatic ecosystem processes. Yet, the dearth of morphological and molecular information hinders the assessment of their diversity and ecological role. Using single-cell techniques, we characterise morphologically and molecularly,...
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ftarchimer:oai:archimer.ifremer.fr:58135 2023-05-15T17:30:52+02:00 Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation Garvetto, Andrea Nezan, Elisabeth Badis, Yacine Bilien, Gwenael Arce, Paola Bresnan, Eileen Gachon, Claire M. M. Siano, Raffaele 2018-12 application/pdf https://archimer.ifremer.fr/doc/00470/58135/60593.pdf https://archimer.ifremer.fr/doc/00470/58135/60594.fasta https://archimer.ifremer.fr/doc/00470/58135/60595.fasta https://archimer.ifremer.fr/doc/00470/58135/60596.fasta https://archimer.ifremer.fr/doc/00470/58135/60597.fasta https://archimer.ifremer.fr/doc/00470/58135/60598.pdf https://archimer.ifremer.fr/doc/00470/58135/60599.pdf https://archimer.ifremer.fr/doc/00470/58135/60600.pdf https://archimer.ifremer.fr/doc/00470/58135/60601.pdf https://archimer.ifremer.fr/doc/00470/58135/60602.pdf https://archimer.ifremer.fr/doc/00470/58135/60603.pdf https://archimer.ifremer.fr/doc/00470/58135/60604.pdf https://doi.org/10.3389/fmicb.2018.02918 https://archimer.ifremer.fr/doc/00470/58135/ eng eng Frontiers Media Sa info:eu-repo/grantAgreement/EC/FP7/230865/EU//DIMBA https://archimer.ifremer.fr/doc/00470/58135/60593.pdf https://archimer.ifremer.fr/doc/00470/58135/60594.fasta https://archimer.ifremer.fr/doc/00470/58135/60595.fasta https://archimer.ifremer.fr/doc/00470/58135/60596.fasta https://archimer.ifremer.fr/doc/00470/58135/60597.fasta https://archimer.ifremer.fr/doc/00470/58135/60598.pdf https://archimer.ifremer.fr/doc/00470/58135/60599.pdf https://archimer.ifremer.fr/doc/00470/58135/60600.pdf https://archimer.ifremer.fr/doc/00470/58135/60601.pdf https://archimer.ifremer.fr/doc/00470/58135/60602.pdf https://archimer.ifremer.fr/doc/00470/58135/60603.pdf https://archimer.ifremer.fr/doc/00470/58135/60604.pdf doi:10.3389/fmicb.2018.02918 https://archimer.ifremer.fr/doc/00470/58135/ info:eu-repo/semantics/openAccess restricted use Frontiers In Microbiology (1664-302X) (Frontiers Media Sa), 2018-12 , Vol. 9 , N. 2918 , P. 19p. Pseudo-nitzschia diatoms marine oomycetes plankton parasites single-cell analysis metabarcoding text Publication info:eu-repo/semantics/article 2018 ftarchimer https://doi.org/10.3389/fmicb.2018.02918 2021-09-23T20:31:51Z Parasites are key drivers of phytoplankton bloom dynamics and related aquatic ecosystem processes. Yet, the dearth of morphological and molecular information hinders the assessment of their diversity and ecological role. Using single-cell techniques, we characterise morphologically and molecularly, intracellular parasitoids infecting four potentially toxin-producing Pseudo-nitzschia and one Melosira species on the North Atlantic coast. These sequences define two, morphologically indistinguishable clades within the phylum Oomycota, related to the genera of algal parasites Anisolpidium and Olpidiopsis and the diatom parasitoid species Miracula helgolandica. Our morphological data are insufficient to attribute either clade to the still unsequenced genus Ectrogella; hence it is proposed to name the clades OOM_1 and OOM_2. A screening of global databases of the barcode regions V4 and V9 of the 18S rDNA demonstrate the presence of these parasitoids beyond the North Atlantic coastal region. During a biweekly metabarcoding survey (Concarneau Bay, France), reads associated with one sequenced parasitoid coincided with the decline of Cerataulina pelagica bloom, whilst the other parasitoids co-occurred at low abundance with Pseudo-nitzschia. Our data highlight a complex and unexplored diversity of the oomycete parasitoids of diatoms and calls for the investigation of their phenology, evolution, and potential contribution in controlling their host spatial-temporal dynamics. Article in Journal/Newspaper North Atlantic Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Frontiers in Microbiology 9 |
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Open Polar |
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Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Pseudo-nitzschia diatoms marine oomycetes plankton parasites single-cell analysis metabarcoding |
spellingShingle |
Pseudo-nitzschia diatoms marine oomycetes plankton parasites single-cell analysis metabarcoding Garvetto, Andrea Nezan, Elisabeth Badis, Yacine Bilien, Gwenael Arce, Paola Bresnan, Eileen Gachon, Claire M. M. Siano, Raffaele Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation |
topic_facet |
Pseudo-nitzschia diatoms marine oomycetes plankton parasites single-cell analysis metabarcoding |
description |
Parasites are key drivers of phytoplankton bloom dynamics and related aquatic ecosystem processes. Yet, the dearth of morphological and molecular information hinders the assessment of their diversity and ecological role. Using single-cell techniques, we characterise morphologically and molecularly, intracellular parasitoids infecting four potentially toxin-producing Pseudo-nitzschia and one Melosira species on the North Atlantic coast. These sequences define two, morphologically indistinguishable clades within the phylum Oomycota, related to the genera of algal parasites Anisolpidium and Olpidiopsis and the diatom parasitoid species Miracula helgolandica. Our morphological data are insufficient to attribute either clade to the still unsequenced genus Ectrogella; hence it is proposed to name the clades OOM_1 and OOM_2. A screening of global databases of the barcode regions V4 and V9 of the 18S rDNA demonstrate the presence of these parasitoids beyond the North Atlantic coastal region. During a biweekly metabarcoding survey (Concarneau Bay, France), reads associated with one sequenced parasitoid coincided with the decline of Cerataulina pelagica bloom, whilst the other parasitoids co-occurred at low abundance with Pseudo-nitzschia. Our data highlight a complex and unexplored diversity of the oomycete parasitoids of diatoms and calls for the investigation of their phenology, evolution, and potential contribution in controlling their host spatial-temporal dynamics. |
format |
Article in Journal/Newspaper |
author |
Garvetto, Andrea Nezan, Elisabeth Badis, Yacine Bilien, Gwenael Arce, Paola Bresnan, Eileen Gachon, Claire M. M. Siano, Raffaele |
author_facet |
Garvetto, Andrea Nezan, Elisabeth Badis, Yacine Bilien, Gwenael Arce, Paola Bresnan, Eileen Gachon, Claire M. M. Siano, Raffaele |
author_sort |
Garvetto, Andrea |
title |
Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation |
title_short |
Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation |
title_full |
Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation |
title_fullStr |
Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation |
title_full_unstemmed |
Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation |
title_sort |
novel widespread marine oomycetes parasitising diatoms, including the toxic genus pseudo-nitzschia: genetic, morphological, and ecological characterisation |
publisher |
Frontiers Media Sa |
publishDate |
2018 |
url |
https://archimer.ifremer.fr/doc/00470/58135/60593.pdf https://archimer.ifremer.fr/doc/00470/58135/60594.fasta https://archimer.ifremer.fr/doc/00470/58135/60595.fasta https://archimer.ifremer.fr/doc/00470/58135/60596.fasta https://archimer.ifremer.fr/doc/00470/58135/60597.fasta https://archimer.ifremer.fr/doc/00470/58135/60598.pdf https://archimer.ifremer.fr/doc/00470/58135/60599.pdf https://archimer.ifremer.fr/doc/00470/58135/60600.pdf https://archimer.ifremer.fr/doc/00470/58135/60601.pdf https://archimer.ifremer.fr/doc/00470/58135/60602.pdf https://archimer.ifremer.fr/doc/00470/58135/60603.pdf https://archimer.ifremer.fr/doc/00470/58135/60604.pdf https://doi.org/10.3389/fmicb.2018.02918 https://archimer.ifremer.fr/doc/00470/58135/ |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Frontiers In Microbiology (1664-302X) (Frontiers Media Sa), 2018-12 , Vol. 9 , N. 2918 , P. 19p. |
op_relation |
info:eu-repo/grantAgreement/EC/FP7/230865/EU//DIMBA https://archimer.ifremer.fr/doc/00470/58135/60593.pdf https://archimer.ifremer.fr/doc/00470/58135/60594.fasta https://archimer.ifremer.fr/doc/00470/58135/60595.fasta https://archimer.ifremer.fr/doc/00470/58135/60596.fasta https://archimer.ifremer.fr/doc/00470/58135/60597.fasta https://archimer.ifremer.fr/doc/00470/58135/60598.pdf https://archimer.ifremer.fr/doc/00470/58135/60599.pdf https://archimer.ifremer.fr/doc/00470/58135/60600.pdf https://archimer.ifremer.fr/doc/00470/58135/60601.pdf https://archimer.ifremer.fr/doc/00470/58135/60602.pdf https://archimer.ifremer.fr/doc/00470/58135/60603.pdf https://archimer.ifremer.fr/doc/00470/58135/60604.pdf doi:10.3389/fmicb.2018.02918 https://archimer.ifremer.fr/doc/00470/58135/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.3389/fmicb.2018.02918 |
container_title |
Frontiers in Microbiology |
container_volume |
9 |
_version_ |
1766127998066491392 |